Enteroviral polynucleotides, methods of detecting enteroviruses and kits containing the polynucleotides

ABSTRACT

The present invention provides polynucleotides useful for detecting and/or typing Enteroviruses contained in a sample by amplification and/or hybridization and/or sequencing; and kits containing the polynucleotides.

FIELD OF THE INVENTION

[0001] The present invention relates to polynucleotides and probes useful for detecting and/or serotyping enteroviruses.

BACKGROUND OF THE INVENTION

[0002] Human enteroviruses (HEV) belong to the Picornaviridae family. They are major human pathogens and are associated with a broad spectrum of clinical features including acute respiratory illness, aseptic meningitis, meningoencephalitis, myocarditis, hand-foot-and-mouth disease, neonatal multi-organ failure and acute flaccid paralysis (Melnick, 1996). Outbreaks of disease associated with a single serotype of enterovirus are often reported (Gjoen et al., 1996, Kopecka et al., 1995) and represent a major public health problem. In 1998, an outbreak of enterovirus 71 infection caused hand-foot-and-mouth disease and herpangina in more than 100,000 individuals in Taiwan, with hundreds of deaths due to complications including encephalitis, aseptic meningitis, pulmonary oedema or hemorrhagic, acute flaccid paralysis and myocarditis (Ho et al., 1999). HEV are currently responsible for 80 to 92% of aseptic meningitis cases with an identified aetiologic agent (Rotbart, 1995).

[0003] The global eradication of poliomyelitis will be soon achieved, vaccination with live oral poliovirus vaccine will be stopped and the ecological niche taken by poliovirus will become vacant. Interest has increased in the circulation, detection, identification and evolutions of non-polio enteroviruses and the emergence of new epidemic strains. The traditional classification of enteroviruses is based on antigenic specificity, as determined by the serum neutralization assay, with all the disadvantages that this method implies.

[0004] Based on their antigenic properties, the original sixty-four HEV serotypes were initially grouped into Polioviruses (PV), Coxsackieviruses A (CA), Coxsackieviruses B (CB), Echoviruses (E), and the more recently identified Enterovirus (EV) 68 to 71. A new HEV classification based on molecular and biological data has recently been proposed as an alternative to the antigenic classification (Hyypia et al., 1997, Poyry et al., 1996). This classification groups enteroviruses into 5 species: 1)-PV, including poliovirus type 1, 2 and 3, 2)-HEV-A, including 11 Coxsackieviruses A and EV71; 3)-HEV-B, including all Coxsackieviruses B, all Echoviruses, EV69 and CA9; 4)-HEV-C including 11 other Coxsackieviruses A; and 5)-HEV-D including EV68 and EV70 (Pringle, 1999). The enteroviruses previously classified as E22 and E23, which were shown to group independently (Hyypia et al., 1992), now constitute a new genus Parechovirus (with two serotypes) in the Picornaviridae family (Miiyo & Pringle, 1998).

[0005] Enterovirus typing is required for several main reasons: i) to distinguish polio- from non-polio-enteroviruses in the context of poliomyelitis eradication, ii) to determine the relationship between enterovirus type and clinical syndrome, iii) to identify new enterovirus types or variants, iv) to analyze enteroviruses in neonates and immunodeficient patients, and v) to investigate enterovirus molecular epidemiology and phylogeny (reviewed in Muir et al., 1998). The agent responsible for HEV-induced diseases is currently identified by conventional virus isolation followed by neutralization with intersecting pools of type-specific antisera. Due to the large number of antigenically distinct serotypes, serotyping procedures are time-consuming, labor-intensive and costly. Moreover, the limited supply of reference type-specific sera, the limited number of serotypes, covered by the intersecting pools of sera currently available (LBM or RIVM), their “static” character (the inability to detect new antigenic variants or emerging serotypes) are also major drawbacks of neutralism typing (el-Sageyer et al., 1998). In addition, enteroviruses are frequently found to be “untypeable”.

[0006] In the light of recent developments in molecular biology, several assays based on the reverse transcription-polymerase chain reaction (RT-PCR) followed by nucleic acid hybridization or sequencing have been assessed as possible approaches for the identification of enteroviruses. Accordingly, genomic sequencing has made it possible to develop various molecular approaches for enterovirus identification and genealogical studies. The enterovirus genome is a single-stranded, positive RNA molecule, approximately 7,500 nucleotides long, including a 5′ and a 3′ non-coding region (NCR), and encompassing a single, long open reading frame. Sets of primers specific for highly conserved sequences in the 5′NCR or VP2 capsid protein-coding regions have been used to develop efficient methods for the rapid and sensitive detection of enteroviruses (reviewed by Romero, 1999). However, neither the 5′NCR nor the VP2 coding region (Oberste et al., 1998) can be used for enterovirus typing, due to a lack of correlation between the nucleotide sequence of these genomic regions and serotype. Accordingly, this use made it possible to detect HEV, but not to identify them beyond the genus level. VP1 is a capsid protein located mainly at the virion surface. It makes a large contribution to the constitution of neutralization antigenic sites. For this reason, the region of the genome encoding VP1 has been used to investigate the molecular evolution of poliovirus (Kew et al., 1995), to determine poliovirus genotypes (Balanant et al., 1991) and to develop poliovirus serotype-specific PCR primers (Kilpatrick et al., 1998). The nucleotide sequence of the entire VP1 coding region has recently been shown to correlate with serotype in enteroviruses (Oberste et al., 1999a) and to contain serotype-specific information in HEV (Oberste et al, 1999b) opening up possibilities for the development of molecular methods for the identification of traditional HEV serotypes and the newly designated HEV species (Pringle, 1999) and of molecular diagnostic reagents for serotype-specific enterovirus identification.

[0007] Here is presented a new approach for the molecular detection and/or serotyping of HEV and for epidemiological studies of HEV, involving the amplification of a genomic fragment encompassing the VP1-2C coding region with a single pair of enterovirus-specific primers. Restricted analysis of the 3′ third of the VP1-coding region showed a good correlation between nucleotide sequence and enterovirus serotype, for both classical reference strains and field isolates, over a 30-year period, and covering widely dispersed geographic regions. Especially, it is here demonstrated that a genomic segment of 364 nucleotides (relative to VP1 Mahoney, Kitamura et al, 1981) of the 3′ third of the VP1-coding region of HEV genomes contains enough serotype-specific information to be used for HEV serotype identification. This method may improve diagnosis of the diseases caused by Enterovirus infection. The amplification of this long genomic fragment may also be used as a rapid and efficient tool for studies of HEV molecular epidemiology and evolution.

[0008] To explore further the phylogenetic relationships between human enteroviruses and to develop new diagnostic approaches, the inventors designed a pair of generic primers for the study of a 1452 bp genomic fragment (relative to Mahoney poliovirus genome), including the 3′ end of VP1-, the 2A- and 2B-, and the 5′ moiety of 2C-coding region. Fifty-nine of the sixty-four prototype strains and forty-five field isolates of various origins, involving 21 serotypes; and six strains untypeable by standard immunological techniques, were successfully amplified with these primers. By determining the nucleotide sequence of the genomic fragment encoding the C-terminal third of the VP1 capsid protein the inventors developed a molecular typing method based on RT-PCR and sequencing. If field isolate sequences were compared to human enterovirus VP1 sequences available in databases, nucleotide identity score was, in each case, highest with the homotypic prototype (74.8 to 89.4%). Phylogenetic trees were generated from alignments of partial VP1 sequences by several phylogeny algorithms. In all cases, the new classification of enteroviruses into five identified species was confirmed and strains of the same serotype were always monophyletic. Analysis of the results confirmed that the 3′ third of the VP1 coding sequence contains serotype-specific information and can be used as the basis of an effective and rapid molecular typing method. Furthermore, the amplification of such a long genomic fragment, including non-structural regions, is straightforward and could be used to investigate genome variability and to identify recombination breakpoints or specific attributes of pathogenicity.

[0009] Accordingly, an RT-PCR amplicon including the 3′ third of the VP1 coding region was obtained for 59 of the 64 reference and all of the 45 field isolates, using an original single pair of universal HEV-specific primers, flanking the 1452 nucleotide VP1-2C coding region (relative to PV1-Mahoney strain). This is the first time that it has been possible to detect and to identify 92.2% of all known prototype HEV with a single pair of primers. In another study (Oberste et al., 1999a), a set of degenerate deoxyinosine-containing PCR primers designed to amplify the region encoding VP1 region was shown to be effective for only 65% of enterovirus prototype strains. The same authors (Oberste et al., 2000) had to design five different pairs of primers to amplify 54 different field isolates. In the present study, the 5 prototype strains not recognized by the pair of primers of the invention have characteristics unusual among HEV. Indeed, only 80% and 70% identity with the sense primers was observed for the CA5, CA19 and CA22 group and for the EV68 and EV70 groups, respectively. These strains are also known to have other characteristics different from those of the other HEV: the three CA viruses thrive only in suckling mice and the two EV constitute a separate species (HEV-D).

BRIEF DESCRIPTION OF THE DRAWINGS

[0010]FIG. 1: Phylogenetic tree showing relationships between the 45 enterovirus field isolates, based on the C-terminal third of the VP1-coding region sequence alignment. The prototype enterovirus representative of each species are indicated in bold. The tree was constructed by the neighbor-joining method with the maximum-likelihood method of Kishino & Hasegawa (Kishino, H. and Hasegawa, M., 1989) with a transition/transversion ratio of 8.0 for the distance matrix. Numbers at nodes represent the percentage of 100 bootstrap pseudoreplicates. The human enterovirus species (Poliovirus, HEV-A, HEV-B, HEV-C and HEV-D) are indicated on the right side by vertical bars (distances between bars are arbitrary). Horizontal branch legends are proportional to the number of substitutions as indicated by the scale (the scale represents 0.1 nucleotide substitutions per site).

[0011]FIG. 2: Phylogenetic trees depicting genetic relationships between field human enteroviruses and prototype HEV-A (a), HEV-C + poliovirus (b), and HEV-B (c). Trees were based on sequence alignment of the C-terminal third of the VP1-coding region and constructed by neighbor-joining method with the maximum-likelihood method of Kishino & Hasegawa (Kishino, H. and Hasegawa, M., 1989) with a transistion/transversion ratio of 8.0 for the distance matrix. Numbers at node represent the percentage of 100 bootstrap pseudoreplicates. Branch lengths are proportional to the number of substitutions as indicated by the scale (the scale bar represents 0.1 nucleotide substitutions per site). Human enteroviruses sequenced in this work are shown in bold.

SUMMARY OF THE INVENTION

[0012] An object of the present invention is to provide polynucleotides which are capable of amplifying and detecting an Enterovirus nucleic acid in an easy, rapid and specific manner with a high sensitivity. To be more specific, the present invention is to provide amplifying primers and also a capturing probe or a detecting probe for identifying the amplified product obtained by said primers whereby the problems encountered in the conventional clinical test methods for Enteroviruses have now been solved.

[0013] Accordingly, an object of the present invention is a purified polynucleotide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 or from the group consisting of SEQ ID NOS:7-51. These sequences include those sequences which stringently hybridizes to one or more of theses polynucleotides.

[0014] A further object of the present invention is a method of detecting an Enterovirus in a sample comprising contacting a polynucleotide region of a VP1-2C gene of said enterovirus with amplification primers; amplifying said polynucleotide region of the VP1-2C genes; and detecting the presence of an amplified polynucleotide, wherein the presence of the amplified product is indicative of the presence of the Enterovirus in the sample.

[0015] In such a method, the amplification comprises reverse transcription and is named RT-PCR.

[0016] Another object of the present invention is a method of detecting the presence of an Enterovirus in a sample, comprising: contacting an isolated and purified nucleic acid comprising (a) a polynucleotide sequence contained in the VP1-2C gene of said Enterovirus with the sample containing (b) an enterovirus; detecting the presence or absence of hybridization between (a) and (b), wherein the presence of hybridization between (a) and (b) is indicative of the presence of the enterovirus in the sample.

[0017] These polynucleotide sequences are selected from the group consisting of SEQ ID NO:7-51 or fragments thereof or sequences hybridizing with one or more of sequences SEQ ID NOS:7-51 or fragments thereof.

[0018] Another object of the present invention is a method of detecting the presence of an Enterovirus in a sample, comprising: contacting a polynucleotide region of VP1-2C genes of said enterovirus with amplification primers; amplifying said polynucleotide region of the VP1-2C genes; sequencing said amplified polynucleotide region; and comparing the sequence of said amplified polynucleotide region with known sequences of enterovirus of various serotypes, wherein an identity between score of at least 75% indicates that the two compared sequences are from enteroviruses of the same serotype.

[0019] In the present application, identity means that the nucleotides of the compared sequences are identical and identically placed in the sequences. An identity score (also called percent homology) of at least 75% between two sequences indicates that such sequences are from enteroviruses of the same serotype. The percent homology can be determined with the GCG sequence analysis software, Genetics Computer Group, Madison, Wis.

[0020] Another object of the present invention is to provide kits suitable for detecting and/or serotyping enterovirus. Such kits include one or more of polynucleotide sequences selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6; and SEQ ID NO:7-51.

[0021] Another object of the present invention is a purified polynucleotide of VP1-2C genes produced by the above-method with or without amplification primers and the purified polypeptides encoded by such a purified polynucleotide.

DETAILED DESCRIPTION OF THE INVENTION

[0022] The present invention provides a method of detecting the presence of an enterovirus in a sample. The method comprises amplifying the viral RNA from the sample using a purified polnucleotide/purified nucleic acid capable of selectively hybridizing with the enterovirus nucleic acid said isolated polynucleotide being selected from the group of the nucleotide sequences set forth in the Sequence Listings as SEQ ID NOS:1-6 (the primers in Table 1: EUC2, EUC2a, EUC2b, EUG3a, EUG3b and EUG3c, respectively), or the sequence complementary thereto and detecting the presence of amplification, the presence of amplification indicating the presence of the Enterovirus in the sample. Detecting the presence of amplification can be conducted by any technique known in the art such as electrophoresis. In addition, the Enterovirus can be detected and/or serotyped by hybridization with one or more of SEQ ID NOS:7-51(enteroviruses 1-45, respectively in Tables 2 and 3) or a fragment of said sequence, such a fragment is preferably chosen in the 3′ third of VP1.

[0023] The terms “polynucleotide” and “nucleic acid” are used herein according to the meaning known in the art. For example, DNA and/or RNA molecules composed of various nucleotides and/or nucleotide analogs. When the molecule is composed of a number of nucleotides of less than 30, they can also be named oligonucleotides.

[0024] The term “fragments” as it relates to polynucleotides and nucleic acid molecules is understood to mean those fragments which are specific for enteroviruses when used for methods to identify the presence of an enterovirus and specific for an enterovirus serotype when used in a method for serotyping an enterovirus.

[0025] The term “molecular serotyping” as used herein is understood to mean serotyping by one of molecular techniques rather than one of usual immunological techniques.

[0026] A “polypeptide” as used herein is understood to mean a sequence of several amino acid residues linked by a peptide bond. Such amino acids are known in the art and encompass the unmodified and modified amino acids. In addition, the polypeptide may be modified by one or modifications known in the art such as glycosylation, phosphorylation, etc.

[0027] The enterovirus can also be detected and/or serotyped by sequencing of its VP1-2C genes and comparing the obtained sequences with known sequences of VP1-2C genes. Methods of sequencing nucleic acids are known in the art and are described in, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York (1989) and Current Protocols in Molecular Biology, Ausebel et al, eds., John Wiley and Sons, Inc., New York (2000).

[0028] Examples of Enteroviruses viruses capable of providing a target nucleic acid sequence for amplification and detection by the method of the present invention include polioviruses, group A coxsackieviruses, group B coxsackieviruses, echoviruses and other newly identified enteroviruses. Preferred Enterovirus DNA targets include the VP1 gene, and particularly the 3′ third of the VP1 gene.

[0029] The viruses can be isolated from any known species of animal which carry Enteroviruses. Preferably the animals are human. The viruses can be contained in any sample obtainable from the animal, for example, saliva, blood, urine and stool, muscle, liver, thymus, cerebrospinal fluid samples and any other product of animal origin. The samples can be subject to purifying protocols known in the art or used in the detection analysis directly. For example, column chromatography, density centrifugation, or ammonium sulfate precipitation. These and other methods are disclosed, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York (1989). For simplicity and ease of assay, it is preferred that the sample be used directly without purification.

[0030] The nucleic acid specific for the Enteroviruses can be detected utilizing a nucleic acid amplification technique, such as polymerase chain reaction (PCR) or ligase chain reaction (LCR). Alternatively, the nucleic acid is detected utilizing direct hybridization or by utilizing a restriction fragment length polymorphism.

[0031] Hybridization can be carried out either using the RNA contained in the viruses or the viral genome may be reverse transcribed prior to amplification. PCR primers which hybridize only with nucleic acids specific for the Enterovirusare utilized. The presence of amplification indicates the presence of the virus. In another embodiment a restriction fragment of a nucleic acid sample can be sequenced, directly using techniques known in the art and described herein, and compared to the known unique sequence to detect the Enterovirus. The present invention also contemplates a method of detecting the presence of the Enterovirus by selective amplification by the methods described herein. Alternatively, the Enterovirus can be detected by directly hybridizing the unique sequence with a nucleic acid probe selective for the Enterovirus. Furthermore, the nucleotide sequence could be amplified prior to hybridization by the methods described above. Such hybridization protocols are known in the art and are disclosed, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York (1989). Such a sequence is, for example, the sequence of an Enterovirus amplified by the primers having the sequences in SEQ ID NO:1-6.

[0032] As used herein stringent hybridization conditions are those conditions which allow hybridization between polynucleotides that are 75%, 80%, 85%, 90%, 95%, or 98% as determined using conventional homology programs, an example of which is UWGCG sequence analysis program available from the University of Wisconsin. (Devereaux et al., Nucl. Acids Res. 12: 387-397 (1984)). Such stringent hybridization conditions typically include washing the hybridization in 2×SSC and 0.5% SDS at 65° C. (Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York (1989)). Of course, one of skill in the art will recognize that conditions can be varied depending on the length of the polynucleotides to be hybridized and the GC content of the polynucleotides see, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York (1989).

[0033] Since Enterovirus have an RNA genome, it is preferred to first amplify the viral genetic material by reverse transcriptase PCR (RT-PCR) to generate a DNA template suitable for sequencing or hybridization. Primers useful for RT-PCR include primers SEQ ID NOS:1-6 which can amplify a region of the Enterovirus, and preferably, the VP1-2C region. Repeated cycles of denaturation, primer annealing and extension carried out with polymerase, e.g., a heat stable enzyme Taq polymerase, leads to exponential increases in the concentration of desired nucleic acid sequences. The nucleic acid can be denatured at high temperatures (e.g., 95° C.) and then reannealed in the presence of a large molar excess of oligonucleotide. The oligonucleotide, oriented with their 3′ ends pointing towards each other, hybridize to opposite strands of the target sequence and prime enzymatic extension along the nucleic acid template in the presence of the four deoxyribonucleotide triphosphates. The end product is then denatured again for another cycle. After this three-step cycle has been repeated several times, amplification of a nucleic acid segment by more than one million-fold can be achieved.

[0034] PCR may be followed by restriction endonuclease digestion with subsequent analysis of the resultant products. Nucleotide substitutions can result in the gain or loss of specific restriction endonuclease site. The gain or loss of a restriction endonuclease recognition site facilitates the detection of the organism using restriction fragment length polymorphism (RFLP) analysis or by detection of the presence or absence of a polymorphic restriction endonuclease site in a PCR product that spans the sequence of interest.

[0035] Primers for PCR, RT-PCR and LCR are usually about 20 bp in length and preferably are from 15 to 25 bp. Better amplification is obtained when both primers are the same length and with roughly the same nucleotide composition. Denaturation of strands usually takes place at 94° C. and extension from the primers is usually at 72° C. The annealing temperature varies according to the sequence under investigation. Examples of reaction times are: 20 mins denaturing; 35 cycles of 2 min, 1 min, 1 min for annealing, extension and denaturation; and finally, a 5 min extension step. Other conditions may be used and will depend on the target, primers, type of sample, etc. Amplification protocols are disclosed, for example, in Innis et al., PCR Protocols, a Guide to Methods and Applications, eds., Academic Press (1990)).

[0036] In this invention “primer” means a polynucleotide which is produced synthetically or biologically and includes a specific nucleotide sequence which permits hybridization to a section containing the target nucleotide sequence. Defined primers/polynucleotides may be produced by any of several well known methods, including automated solid-phase chemical synthesis using cyanoethylphosphoramidite precursors. Other well-known methods for construction of synthetic primers/oligonucleotides may, of course, be employed. 2 J. Sambrook, E. F. Fritsch and T. Maniatis, Molecular Cloning 11 (2d ed. 1989).

[0037] The primers used to amplify the sample nucleic acids may be coupled to a detectable moiety. A preferred example of such a detectable moiety is fluorescein, which is a standard label used in nucleic acid sequencing systems using laser light as a detection system. Other detectable labels can also be employed, however, including other fluorophores, radio labels, chemical couplers such as biotin which can be detected with streptavidin-linked enzymes, and epitope tags such as digoxigenin detected using antibodies. The primers may be modified whereby another nucleotide is added to or substituted for at least one nucleotide in the oligonucleotide. The term “add(ed)” means that nucleotide, oligo dGTP, oligo DATP, oligo dTTP, oligo dCTP, etc. having fluorescence substance, linker arm, biotin, etc. are bound to a 5′-terminal or a 3′-terminal of the oligonucleotide sequence. The term “substitute(d)” means that nucleotide having fluorescence substance, linker arm, biotin, etc. is introduced as a substitute for at least one nucleotide in the oligonucleotide. Introduction of known labels such as radioactive substances, enzymes, fluorescence substances, etc. after synthesis of oligonucleotide is also included therein.

[0038] Examples of suitable primers for reverse-transcription and amplification in the present method are shown in Table 1 (SEQ ID NOS:1-6).

[0039] For example, primers are from about 15 to 25 nucleotides in length, without internal homology or primer-primer homology. It is also desirable for the primers to form more stable duplexes with the target DNA at the primer's 5′-ends than at their 3′-ends, because this reduces false priming. Stability can be approximated by GC content, since GC base pairs are more stable than AT pairs, or by nearest neighbor thermodynamic parameters. Breslauer et al., “Predicting DNA duplex stability from base sequence”, Proc. Nat'l Acad. Sci. USA 83: 3746-3750 (1986).

[0040] Additional factors to the selection of primers for amplification are discussed in Rylchik, W., Selection of Primers for Polymerase Chain Reaction”, in Methods in Molecular Biology, Vol. 15: PCR Protocols: Current Methods and Applications, White, B. A. ed., Humana Press, Totowa, N.J., 1993. Primer pairs are selected by position, similarity of melting temperature, internal stability, absence of internal homology or homology to each other and the 3′-end will not form a stable hairpin loop back on itself.

[0041] To evaluate compatibility of primers for use in amplification, it is desirable to determine the predicted melting temperature for each primer. This can be accomplished in several ways. For example, the melting temperature (Tm) can be calculated using either of the following equations:

Tm(°C.)=81.5+16.6×log[Na]+0.41×(% GC)−675/length

[0042] where [Na] is the concentration of sodium ions, and the % GC is in number percent,

[0043] or

Tm(°C.)=2×(A+T)+4(G+C)

[0044] where A, T, G, and C represent the number of adenosine, thymidine, guanosine and cytosine residues in the primer. Preferably, primers for coamplification should be selected to have predicted melting temperatures differing by less than 4° C.

[0045] The present invention employs one or more polymerizing enzymes capable of polymerizing deoxyribonucleoside triphosphates (and modified deoxyribonucleoside triphosphates) into a complementary strand of DNA using an amplification primer and a target nucleic acid sequence as a template. Examples of such enzymes are Avian Myeloblastosis Virus Reverse Transcriptase (AMV-RT), Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT), Superscript II (Life Technologies, Inc., Rockville, Md.), and DNA polymerases such as E. coli DNA polymerase, E. coli DNA polymerase (Klenow fragment), ^(exo-) E. coli DNA polymerase (Klenow fragment), Bst DNA polymerase, Taq DNA polymerase, Tfl DNA polymerase, and Tth DNA polymerase.

[0046] The amplification reaction may be carried out for a limited number of amplification cycles. It will be understood, that the more cycles of amplification are carried out, the more of the desired product will be made and thus the easier its detection will be. It should also be recognized, however, that during the initial cycles (generally the first 20-25 cycles), the amount of DNA of the desired sequence doubles in each cycle, while thereafter the yield of desired product per cycle drops off. For maximum effectiveness in the method of the present invention, the amplification of the target nucleic acid should be carried out only for a number of cycles during which doubling of DNA is still being achieved.

[0047] The present invention further features a kit that incorporates the components of the invention and makes possible convenient performance of the invention. Such a kit may also include other materials that would make the invention a part of other procedures, and may also be adaptable for multi-well technology. The kit is useful for amplification, detection, typing, or combination thereof for the Enteroviruses presumed to be contained in a sample.

[0048] Preferred components of the kit(s) include any one of the inventive polynucleotide sequences, either the primers used for amplification possibly comprising reverse transcription, e.g., SEQ ID NOS:1-6 or the polynucleotide containing the sequence amplified, e.g, SEQ ID NO:7-51 or fragments thereof, such fragments being specific of the serotype when the kit is used for serotyping of enteroviruses and specific for enteroviruses when used for methods to identify the presence of an enterovirus in a sample. Other suitable kit components include, enzymes used in amplification or hybridization, buffers for diluting the sample to be tested, and reagents for detecting the amplified products and/or hybridized samples. The polynucleotides contained in the kit can be modified with detectable moieties prior to inclusion into the kit or include reagents suitable for modifying the polynucleotides with detectable moieties as is known in the art.

[0049] To avoid missing the detection of an enterovirus by our RT-PCR assay, an alternative mean of detection should be used. One possibility is to include in the diagnosis procedure the classical enterovirus-specific PCR in the 5′NCR. If detected in this way, an enterovirus which was not amplified with the pair of primers of the invention could be further genotyped with an HEV-D or CA5, 19 and 22-specific primers.

EXAMPLES

[0050] Having generally described this invention, a further understanding can be obtained by reference to certain specific examples which are provided herein for purposes of illustration only, and are not intended to be limiting unless otherwise specified.

[0051] Methods

[0052] Prototype Viruses

[0053] The Echoviruses, Coxsackieviruses B, Coxsackieviruses A and enterovirus types 68 to 71 studied in this work were the ‘prototype’ strains of each serotype (Melnick, 1996) and were kindly supplied by the National Institute of Public Health and the Environment (RIVM), Bilthoven, The Netherlands. The serotype of the reference strains was checked by seroneutralization using the Lim Benyesh-Meinick (LBM) panel of intersecting antisera pools (Melnick et al., 1973).

[0054] Field Isolates

[0055] Forty-five enterovirus isolates were selected, representing 21 serotypes throughout the Enterovirus genus. The panel included 35 virus isolates from Europe (France, Greece, the Netherlands and Romania) and 10 isolates from Africa (Burkina Faso, Madagascar) (Table 2). They were isolated from original clinical specimens: stool (n=31), cerebrospinal fluid (n=4), blood (n=1), cortex (n=1), spinal cord (n=2), nasopharyngeal secretion (n=2), vesicle (n=2) and throat (n=2). Clinical symptoms and the specimens studied in this work are described in Table 2. The serotypes of Romanian isolates were determined by neutralization with the LBM and home-made antisera pools and those of the Dutch, French, Greek, Madagascar isolates were determined by neutralization with the RIVM pools (Kapsenberg, 1988). The 6 Madagascan enterovirus isolates, which were not neutralized by any of the PDVM pools, were classified as “untypeable”.

[0056] RT-PCR

[0057] RNA was extracted from 100 μl of infected cell culture supernatant using the Total Quick RNA Talent Idt (Euromedex, France) and eluted in 70 μl of DEPC-treated water. RNA (2 μl) was used for cDNA synthesis with 10 pmol each of the antisense primers, EUC2a and EUC2b (see Table 1 for the primers, SEQ ID NOS:1-6). The reaction (20 μl) contained 50 mM Tris-HCl pH 8.3, 75 mM KCl, 3 mM MgCl₂, 10 mM dNTPs, 2 mM DTT, 20 U ribonuclease inhibitor (RNasin, Promega) and 200 U Superscript RNase H-reverse transcriptase (Gibco BRL, Life Technologies). The reaction mixture was incubated for 30 min at 42° C. and was then heated for 5 min at 95° C. to inactivate the enzyme. The cDNA product (2 μl) was added to a PCR mixture containing 67 mM Tris-HCl pH 8.8, 16 mM (NH₄)₂SO₄, 0.01% Tween-20, 2 mM MgCl₂ 10 mM dNTPs, 1.25 U of Taq DNA polymerase (EurobioTaq, Eurobio) and 10 pmol of primers (EUC2, EUG3a, EUG3b and EUG3c). Amplification involved 29 cycles of denaturation at 95° C. for 20 s, annealing at 45° C. for 1 min and elongation at 72° C. for 1 min, followed by a final cycle of denaturation at 95° C. for 20 s, annealing at 45° C. for 1 min and elongation at 72° C. for 10 min. Amplification products (5 μl) were run on 1.5% agarose gels. The gels were stained with ethidium bromide and the DNA was viewed under UV light. RT-PCR was used to amplify a 435 bp fragment in the 5′ non-coding region, from 2 μl of purified RNA, as previously described (Balanant et at., 1991), using primers UC52-UG53 (Gufflot et al., 1994).

[0058] Nucleotide Sequence Determination and Sequence Analysis

[0059] PCR products were purified by the low-melting-point agarose gel method (Sambrook et al., 1999) and sequenced on an automated DNA sequencer using the BigDye Terminator Cycle Sequencing Ready Reaction kit (Perldn Elmer Biosystems), with the EUG3a, EUG3b and EUG3c primers. Sequences were compared with the GenBank database sequences using the program FASTA version 3.3 (Pearson & Lipman, 1988).

[0060] Pairwise nucleotide and amino acid sequence identities were calculated by aligning each field isolate sequence with each available prototype enterovirus sequence using the multiple alignment program CLUSTAL W (Thompson et al., 1994). To analyze phylogenetic relationships, the partial VP1 sequences of isolates were compared with those from other human enteroviruses using CLUSTAL W. Some reference enterovirus nucleotide sequences from the GenBank database were used (Oberste et al., 1999b). Alignments were coffected manually to maximize sequence identity, to account for codon boundaries and to ensure the alignment of conserved amino acid motifs. Phylogenetic trees were generated by inputting the aligned sequences into PHYLIP (Phylogeny Inference Package) version 3.5 (Felsenstein, 1993) and PUZZLE version 4.0 (Strimmer & von Haeseler, 1996). Phylogenetic trees were constructed using the neighbor-joining algorithm of Saitou & Nei (1987), as implemented in the program NEIGHBOR, and using the maximum parsimony method, as implemented in DNAPARS. For neighbor-joining analysis, a distance matrix was calculated using the Kishino and Hasegawa method (Kishino & Hasegawa, 1989) with a transition/transversion ratio (k) of 8.0, using DNADIST (PHYLIP). The k parameter is an empirical ratio calculated by PUZZLE from the data set. To investigate the robustness of the phylogenies constructed with NEIGHBOR and DNAPARS, bootstrap analysis was carried out on 100 pseudo-replicate data sets with SEQBOOT. Phylogenic trees were reconstructed by the maximum likelihood method with PUZZLE, which uses QUARTET PUZZLING as the tree search algorithm. Distances were calculated with the model of nucleotide substitutions of Kishino and Hasegawa (Kishino & Hasegawa, 1989) and the transition/inversion parameter was estimated directly from the data set. The reliability of tree topology was estimated by use of 1,000 puzzling steps. The trees were drawn using program TREEVIEW (Page, 1996).

[0061] Nucleotide Sequence Accession Numbers

[0062] The nucleotide sequence data reported in the paper amplification have been submitted to the EMBL sequence database under the accession numbers AJ279 151 to AJ279195 (SEQ ID NOS:7-51 Table 2).

Results

[0063] Selection of Generic Primers and Evaluation for Amplification

[0064] The first goal was to develop a RT-PCR assay capable of detecting all known serotypes of human enteroviruses, facilitating the identification of enteroviruses and phylogenetic study by means of amplicon sequencing. The inventors therefore designed a pair of generic degenerate PCR primers, binding to the sequences on either side of the sequences encoding, VP1 and 2C (Table 1, SEQ ID NOS:1-6). None of the antisense primers contained a mixed-base and none of the sense primers had more than one degenerate position. The amplicon thus obtained was 1452 bp long (relative to the PV I-Mahoney sequence) and included the 3′ end of the VP1 coding sequence, the entire coding sequence of 2A and 2B, and the 5′ moiety of the 2C coding region of the enterovirus genome.

[0065] To determine specificity, an equimolar mixture of primers was first tested in an RT-PCR assay with the RNA extracted from each of the prototype human enteroviruses. Amplicons were obtained from 59 of the 64 prototype human enteroviruses (92.2%). The viruses for which the amplification reaction was unsuccessful were CA5, CA19 and CA22, which thrive only in suckling mice, EV68 and EV70, members of the distant HEV-D species, and E22 and E23, which are now classified as a new genus (Parechovirus) of the Picornaviridae family (Hyypia et al., 1992). The failure of amplification was not due to a low concentration of viral RNA in the reaction, as shown by the successful amplification of an HEV-specific genomic fragment from the 5′ NCR, as for all other HEV strains tested (not shown).

[0066] Amplicons were also successfully obtained from all of the 45 clinical enterovirus isolates tested (Table 3), irrespective of their date of isolation (1970 to 1998), serotype (21 different serotypes), or the geographical region in which they were collected.

[0067] Nucleotide Sequence and Serotypes

[0068] We sequenced 600-700 nucleotides including the 3′ third of the VP1 gene and the beginning of the 2A gene for each isolate using sense EUG3a, EUG3b and EUG3c primers in combination or separately. All new sequence data presented in this work have been published in the EMBL database (Table 2). The partial VP1 sequence of each of the 45 field isolates (a stretch of 365 nucleotides from 3021 to 3385, numbering according to PV1-Mahoney) was compared with all enterovirus sequences published in GenBank. The gbvrl library was used for nucleic acid analysis and the gpvrl library for protein analysis gbvrl and gpvrl libraries are options of Genbank. A hundred percent correlation was obtained between the serotype determined by the 3′ third of the VP1 coding region sequence and the serotype determined by the conventional neutralization assay for all the 45 field isolates of known serotype tested (Table 3).

[0069] For each field isolate tested, identity was highest with the homotypic prototype strain, for both nucleotide and amino acid sequences, with identities of 74.8 to 89.4% for nucleotide sequences and from 89.8 to 98.2% for amino acid sequences (Table 3). In all cases in which a sequence from a more recent homotypic isolate was present in the database, a better score was obtained with this strain than with the homotypic reference strain (not shown). However, in one case (CB5-RO-14/5/70), the VP1 sequence of the isolate was less similar to the homotypic CB5 prototype (78.5%) than to the prototype of swine vesicular disease virus (SVDV), a pig enterovirus (89.2%), with which the highest identity score was obtained. The deduced amino acid sequence of CB5-RO-14/5/70 was 95.5% similar to those of both SVDV and CB5. This is not surprising as it has been shown that SVDV (Swine Venicular Disease virus) probably arose in pigs from a single transfer of a human CB5, and may therefore be considered to be a subspecies of CB5 (Zhang et al., 1999).

[0070] In all cases, the second-highest identity scores with respect to another serotype were 67.9 to 78.5% for nucleotide sequences and 70.4 to 95.5% for amino acid sequences. In each case, the prototype strain giving the second-highest score belonged to the same species as the strain giving the highest score. The range of the delta scores, representing the difference in percentage nucleotide sequence identity between the highest and second highest scores was from 1.4 to 19.4% (Table 3). This difference, demarcating the boundary between serologically homotypic and the closest heterotypic strains, was on average 10.0% but was very low for seven clinical isolates. Isolate E1-RO-122/1/74 had a nucleotide sequence 80.5% similar to that of E1 prototype strain and 77% similar to that of E8 prototype strain. This difference of only 3.5% is consistent with the previously demonstrated antigenic relationship between E1 and E8 (Harris et al., 1973) and the reclassification of E8 as a variant of E1. The other six isolates with a small difference in percentage nucleotide identity between the highest and second-highest scores (1.4 to 2.9%) are, discussed below.

[0071] Analysis of the “Untypeable” Field Enteroviruses

[0072] Strains MG-354/94, MG-356/94, MG-404/94, MG-448/94, MG-423/94 and MG498/94 were all isolated from the stools of healthy Malagasy children (Table 2). They were identified as enteroviruses by their cytopathic effect on an enterovirus-susceptible cell line and by the enterovirus-specific amplification (RT-PCR) of the 5′ non-coding genomic region. They were not neutralized by intersecting RIVM pools. Their genome was successfully amplified by our pair of enterovirus-specific VP1-2C primers and sequenced. The highest and second-highest identity scores obtained by comparing the partial VP1 sequences of these strains with those of HEV strains in the GenBank are reported in Table 3. Isolates MG-354/94, MG-356/94, MG-404/94 and MG-498/94 were 77.4 to 80.2% identical to CA13 (Flores) and 75.4 to 78.0% identical to CA18 (G-13) prototype strains. The high level of sequence identity between these two reference strains (76.0%), consistent with their antigenic relatedness (Committee on Enteroviruses, 1962), may account for the small difference between the two scores (1.4 to 2.2%). By neutralization with several monospecific CA13 antisera (kindly supplied by RIVM), all the above isolates were identified as serotype CA13 (not shown). A similar problem of a small difference between the highest and second-highest scores was encountered for strains MG-448/94 and MG-423/94. Indeed, MG-448/94 was 74.8% identical to CA20 (IH-35) and 71.9% identical to CA17 (G-12) reference strains, with the CA20 and CA17 prototypes displaying only 70.0% sequence identity. Similarly, strain MG-423/94 was 75.0% identical to CA20 and 72.3% identical to CA13, with the CA20 and CA13 prototypes only 71.0% identical to one another. No antigenic cross-reactivity of CA20 with CA17 or CA13 has been reported. Both isolates were identified as CA20 isolates by neutralization with monospecific sera. However, they were strongly neutralized by both anti-CA20 (IH-35) and anti-CA20a (Tulano) antibodies, and less strongly by anti-CA20b (Cecil) antibodies (not shown), reflecting the antigenic relationship of these three variants of CA20 (personal communication from Albert Ras). Thus, in this study, isolates MG-448/94 and MG-423/94 had the lowest percentage nucleotide sequence identity with the closest reference prototype strain (74.8 and 75% respectively). These field strains have probably, accumulated several mutations, causing them to drift away from their homologous prototype, which was isolated in 1955.

[0073] Phylogenetic Relationships of Field Isolates

[0074] To determine the relationships between field and prototype HEV, the sequence of the 3′ third of the VP1-coding region of each field isolate was compared with those of all prototype strains by pairwise alignments, with the program CLUSTAL W. In each case, the homologous serotype pairwise comparison scores were higher than 75% for nucleotide identity and higher than 85% for amino acid identity. Furthermore, there is no overlap between the homologous serotype pairwise comparison scores of both nucleotide and amino acid sequences and the heterologous serotype pairwise comparison scores (data not shown). This confirmed the accuracy of the molecular serotyping: the serotype of every isolate could be determined if the 3′ third of VP1 sequence displayed a minimum of 75% nucleotide identity (85% amino acid identity) with a prototype strain in the database.

[0075] To explore further the evolutionary relationships between field and prototype viruses, a general tree was generated with the 45 isolates and representatives of each of the five identified HEV species (PV:PV1; HEV-A: CA2, CA12 and CA16; HEV-B: E27, CB3, CA9 and EV69; HEV-C: CA13, CA19 and CA24; HEV-D: EV68 and EV70). The same tree topology was produced, regardless of the algorithm used. The isolates clearly segregated into five distinct major groups (FIG. 1), consistent with previously published human enterovirus phylogenies (Huttunen et al., 1996, Oberste et al., 1998, Poyry et al., 1996, Pulli et al., 1995) and the new classification. The various groups were strongly supported by bootstrap values of 100% for HEV-B, C and D and 92% for HEV-A species, regardless of the algorithm used.

[0076] To study more precisely the phylogenetic relationships between field isolates, we constructed phylogenetic trees within each defined species for the clinical isolates and the corresponding prototype HEV. In each tree, one sequence from each of the other species was included as an outgroup. The HEV-D species was not analyzed because no appropriate isolate was available. Within each species, the same general tree topology was obtained irrespective of the method used. However, some variation in the branching order of some subgroups, in bootstrap values for certain nodes and in branch lengths was observed. Whatever the species, all homotypic strains (field and prototype) were monophyletic, as supported by parametric bootstrap analysis. Three subgroups, slightly different from the published subgroups (Oberste et al., 1999b) were observed for HEV-A species (FIG. 2a): (1) CA2, CA6, CA10, CA4, CA8 and CA3; (2) CA7, CA14, CA5 and CA12 and (3) EV71 and the two CA16 isolates. The CA16 isolates were closely related to each other and to their homologous prototype strain. The clustering of CA16 with the prototype EV71 strain (bootstrap value of 91%), is consistent with a prior observation that CA16 has an antigen in common with EV71 (Hagiwara et al., 1978). The HEV-C species was divided into the same 4 subgroups as already published (Oberste et al., 1999b): (1) CA1, CA19 and CA22; (2) CA21 and CA24; (3) CA11 and CA15 and (4) PV1, PV2, PV3, CA17, CA13 prototype isolates, CA18, CA20 prototype and isolates (FIG. 2b). HEV-B species made up the largest group, with 37 isolates of the 45 analyzed belonging to this phylogenetic group. By analyzing 37 field strains and 44 prototype strains, including three outgroup strains (EV70, CA16 and PV1) (FIG. 2c), we found that the field isolates formed monophyletic clades with their prototype, well supported by bootstrap analysis. All the CBVs clustered together, with each isolate close to its prototype. As previously reported, CB2 and CB4 were more related to each other than to the other CB serotypes, whereas the CB1, CB3 and CB5 strains constituted a single subgroup and CB6 formed a branch of its own (Lindberg & Polacek, 2000). In general, the clinical isolates of a given serotype were more closely related to each other than to their homologous prototypes, which were often displayed on a different branch.

[0077] The two E9 prototypes were not clustered together in a single subgroup and the E9RO-1/9/72 isolate was more closely related to the E9/Barty/57 prototype than to the other isolate, E9-RO-116/6/82. The E9-RO-1/9/72 isolate and E9/Barty/57 were very similar. E9-RO-1/9/72 was isolated from the spinal cord of a child with encephalitis and E9/Barty/57 was isolated from the cerebrospinal fluid of a child with aseptic meningitis. The two E9 field strains had an additional 10 amino-acid fragment including an RGD motif in the C-terminal part of the VP1 structural protein (data not shown), which differentiated the pathogenic Barty strain from the non-pathogenic Hill strain (Zimmerman et al., 1996), and has been described as a probable major determinant of virulence (Nelsen-Salz et al., 1999, Zimmermann et al., 1997).

[0078] The neutralization test, the traditional standard procedure for enterovirus identification, is generally reliable but may fail to identify isolates due to mixtures of enteroviruses, the aggregation of virus particles, antigenic drift, or simply because it is impossible to identify all circulating HEV serotypes with the intersecting pools of antisera in current use. With the present system, it was possible to obtain amplicons from all of the 45 field strains, randomly chosen from HEV isolates representing 21 different serotypes, from various geographic regions of the world, spanning a 30-year period. By comparing the 3′ end of VP1 sequence of each isolate with those of all prototype enteroviruses, it was possible to confirm or to identify unambiguously the serotype of all these isolates. Six of the 45 isolates were untypeable with intersecting sera, but were correctly “serotyped” with the present molecular method, the results being confirmed by neutralization with monospecific antisera. It is also possible to determine the serotypes of over one hundred other strains, from 26 different serotypes (not shown).

[0079] The results reported here are consistent with previous findings (Oberste et al., 1999a), showing a good correlation between molecular and antigenic serotyping for HEV. The sequencing of VP1-2C coding region and especially of the 3′ third of the VP1 coding region, or its hybridization with a specific probe is a useful tool for the rapid identification of enteroviruses, for the diagnosis of enterovirus infections, for determining the extent of genotype divergence among isolates of a given serotype and for phylogenetic studies of enteroviruses. Thus, the molecular strategy improves the identification and characterization of enterovirus isolates and constitutes a rational basis for replacing serotyping by easy rapid genotyping.

[0080] The key advantage of the present molecular strategy for the identification of HEV, based on the use -of VP1-2C primers, is that it requires only a single pair of optimized polynucleotides for the serotyping and genotyping of almost all HEV strains, opening up new possibilities for diagnosis purposes, for studying epidemiological or pathological features and for searching for new serotypes of HEV.

[0081] Obviously, numerous modifications and variations on the present invention are possible in light of the above teachings. It is therefore to be understood that within the scope of the appended claims, the invention may be practiced otherwise than as specifically described herein.

[0082] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art of molecular biology. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described herein. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and are not intended to be limiting.

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[0125] Zimmermann, H., Eggers, H. J. & Nelsen-Salz, B. (1996). Molecular cloning and sequence determination of the complete genome of the virulent echovirus 9 strain barty. Virus Genes 12, 149-54.

[0126] Zimmermann, H., Eggers, H. J. & Nelsen-Salz, B. (1997). Cell attachment and mouse virulence of echovirus 9 correlate with an RGD motif in the capsid protein VP1. Virology 233, 149-56. TABLE 1 Primers used for RT-PCR and sequencing of Human Enteroviruses Genomic Primer Sequence* Polarity Gene location† EUC2 5′ TTT GCA CTT GAA CTG TAT GTA 3′ Antisense 2C 4454-4474 EUC2a 5′ GGT TCA ATA CGG CAT TTG GA 3′ Antisense 2C 4469-4488 EUC2b 5′ GGT TCA ATA CGG TGT TTG CT 3′ Antisense 2C 4469-4488 EUG3a 5′ TGG CAA ACT TCC WCC AAC CC 3′ Sense VP1 3002-3021 EUG3b 5′ TGG CAA ACA TCT TCM AAT CC 3′ Sense VP1 3002-3021 EUG3c 5′ TGG CAG ACT TCA ACH AAC CC 3′ Sense VP1 3002-3021

[0127] TABLE 2 Details of enteroviruses isolates used in this study No. Isolate name* Serotype Geographic location Sample origin Pathology Accession no.† 1 RO-14/5/70 CB5 Romania blood meningitis AJ279151 2 RO-79/2/71 E5 Romania stool meningitis AJ279152 3 RO-29/6/72 E11 Romania naso-pharyngeal secretion meningitis AJ279153 4 RO-1/9/72 E9 Romania spinal cord encephalitis AJ279154 5 RO-86/1/73 CB6 Romania stool spinal paralysis AJ279155 6 RO-78/3/74 E12 Romania stool facial paralysis AJ279156 7 RO-122/1/74 E1 Romania stool spinal paralysis AJ279157 8 RO-98/1/74 CB1 Romania stool spinal paralysis AJ279158 9 RO-112/1/78 E8 Romania stool meningitis AJ279159 10 RO-24/9/79 E6 Romania spinal cord meningitis AJ279160 11 RO-81/1/79 E14 Romania stool spinal paralysis AJ279161 12 RO-28/12/79 E6 Romania cortex bronchopneumonia AJ279162 13 RO-609/4/80 CA9 Romania cerebro-spinal fluid meningitis AJ279163 14 RO-434/2/81 E7 Romania stool spinal paralysis AJ279164 15 RO-543/1/81 E11 Romania stool spinal paralysis AJ279165 16 RO-116/6/82 E9 Romania naso-pharyngeal secretion rachialgia AJ279166 17 RO-104/1/82 E7 Romania stool spinal paralysis AJ279167 18 RO-69/1/86 CB4 Romania stool hemiparesis AJ279168 19 NL-16271/87 E11 The Netherlands stool meningitis AJ279169 20 NL-2463/88 E11 The Netherlands stool gastro-intestinal disorder AJ279170 21 NL-9691/88 E11 The Netherlands stool pharyngitis AJ279171 22 NL-8120/88 E11 The Netherlands stool respiratory infection AJ279172 23 RO-69/1/89 CB3 Romania stool spinal paralysis AJ279173 24 FR-2689/91 E30 France stool meningeal syndrome AJ279174 25 RO-38/3/91 CA9 Romania stool spinal paralysis AJ279175 26 FR-1477/93 E25 France stool not known AJ279176 27 GR-KYR/94 CA16 Greece vesicle hand, foot, and mouth disease AJ279177 28 GR-CHR/94 CA16 Greece vesicle hand, foot, and mouth disease AJ279178 29 MG-354/94 ut‡ Madagascar stool healthy AJ279179 30 MG-356/94 ut Madagascar stool healthy AJ279180 31 MG-404/94 ut Madagascar stool healthy AJ279181 32 MG-448/94 ut Madagascar stool healthy AJ279182 33 MG-451/94 CB Madagascar stool healthy AJ279183 34 MG-423/94 ut Madagascar stool healthy AJ279184 35 MG-498/94 ut Madagascar stool healthy AJ279185 36 FR-1254/95 CB France throat not known AJ279186 37 FR-2272/95 CB France throat not known AJ279187 38 RO-141/2/95 E7 Romania stool facial paralysis AJ279188 39 RO-123/1/95 CB3 Romania stool meningo-encephalitis AJ279189 40 FR-3574/96 E30 France cerebro-spinal fluid meningitis AJ279190 41 FR-1829/96 E30 France cerebro-spinal fluid meningitis AJ279191 42 FR-2479/96 E30 France cerebro-spinal fluid meningitis AJ279192 43 MG-41094/97 E11 Madagascar stool acute flaccid paralysis AJ279193 44 BF-01/98 E33 Burkina Faso stool acute flaccid paralysis contact AJ279194 45. MG-44381/98 E7 Madagascar stool acute flaccid paralysis AJ279195

[0128] TABLE 3 Correspondence between partial VP1 sequencing and seroneutralisation Highest-score prototype* Second-highest-score prototype sequence identity sequence identity Delta Score† Isolate Serotype Type % nt % aa Type % nt Type % aa % nt 1 RO-14/5/70 CB5 SVDV 89.2 95.5 CB5 78.5 CB5 95.5 10.7 2 RO-79/2/71 E5 E5 82.7 95.0 E16 71.8 E31 80.3 10.9 3 RO-29/6/72 E11 E11 80.3 94.2 E19 72.0 E19 83.4 08.3 4 RO-1/9/72 E9 E9 89.4 94.3 E16 74.3 E16 78.2 15.1 5 RO-86/1/73 CB6 CB6 79.4 93.7 E21 70.8 CB3 72.3 08.6 6 RO-78/3/74 E12 E12 81.7 95.9 E11 70.0 E11 73.5 11.7 7 RO-122/1/74 E1 E1 80.5 97.3 E8 77.0 E8 92.6 03.5 8 RO-98/1/74 CB1 CB1 81.4 95.3 CB3 71.4 CB3 83.1 10.0 9 RO-112/1/78 E8 E8 83.4 98.2 E1 76.3 E1 94.6 07.1 10 RO-24/9/79 E6 E6 81.4 94.8 E24 73.6 E29 77.4 07.8 11 RO-81/1/79 E14 E14 81.4 92.0 E2 70.6 E31 78.9 10.8 12 RO-28/12/79 E6 E6 81.3 94.0 E24 73.5 E29 77.3 07.8 13 RO-609/4/80 CA9 CA9 80.3 94.6 E11 70.6 E9 70.4 09.7 14 RO-434/2/81 E7 E7 79.1 95.8 E11 68.9 E11 74.1 10.2 15 RO-543/1/81 E11 E11 81.2 93.3 E19 76.5 E19 83.4 04.7 16 RO-116/6/82 E9 E9 83.9 93.4 E5 74.8 E16 78.0 09.1 17 RO-104/1/82 E7 E7 79.4 96.6 E11 68.7 E11 74.7 10.7 18 RO-69/1/86 CB4 CB4 84.9 96.3 CB3 71.5 CB2 77.1 13.4 19 NL-16271/87 E11 E11 80.5 94.2 E19 75.2 E19 84.2 05.3 20 NL-2463/88 E11 E11 79.8 93.3 E19 75.7 E19 84.2 04.1 21 NL-9691/88 E11 E11 81.4 95.0 E19 75.2 E19 84.8 06.2 22 NL-8120/88 E11 E11 81.7 94.2 E19 75.7 E19 83.4 06.0 23 RO-69/1/89 CB3 CB3 81.0 97.2 E13 72.5 CB1 81.6 08.5 24 FR-2689/91 E30 E30 88.4 96.5 E21 69.0 E21 84.0 19.4 25 RO-38/3/91 CA9 CA9 82.4 93.8 E9 67.9 E9 70.4 14.5 26 FR-1477/93 E25 E25 79.6 92.5 E21 72.3 E21 83.4 07.3 27 GR-KYR/94 CA16 CA16 80.8 96.2 EV71 70.0 EV71 76.8 10.8 28 GR-CHR/94 CA16 CA16 78.9 93.8 EV71 68.9 EV71 73.4 10.0 29 MG-354/94 ut CA13 77.9 90.6 CA18 76.3 CA18 89.8 01.6 30 MG-356/94 ut CA13 77.4 89.8 CA18 75.4 CA18 88.9 02.0 31 MG-404/94 ut CA13 80.2 92.3 CA18 78.0 CA18 91.5 02.2 32 MG-448/94 ut CA20 74.8 93.3 CA17 71.9 CA18 82.5 02.9 33 MG-451/94 CB CB6 78.1 92.0 CB3 69.5 E11 70.7 08.6 34 MG-423/94 ut CA20 75.0 90.6 CA13 72.3 CA13 80.3 02.7 35 MG-498/94 ut CA13 78.7 93.2 CA18 77.3 CA18 92.4 01.4 36 FR-1254/95 CB CB1 81.2 98.1 CB3 73.6 CB3 84.2 07.6 37 FR-2272/95 CB CB1 81.3 96.4 CB3 72.4 CB3 84.6 08.9 38 RO-141/2/95 E7 E7 78.7 96.6 E11 68.7 E11 74.7 10.0 39 RO-123/1/95 CB3 CB3 81.1 97.9 CB1 71.7 CB1 80.5 09.4 40 FR-3574/96 E30 E30 88.5 97.5 E21 70.9 E21 84.6 17.6 41 FR-1829/96 E30 E30 89.1 97.5 E21 70.3 E21 84.6 18.8 42 FR-2479/96 E30 E30 89.1 97.5 E21 70.3 E21 84.6 18.8 43 MG-41094/97 E11 E11 79.3 91.8 E19 71.5 E19 83.7 07.8 44 BF-01/98 E33 E33 78.8 95.5 E4 69.0 E19 77.9 09.8 45 MG-44381/98 E7 E7 77.5 95.0 E2 72.4 E11 76.2 05.1

[0129]

0 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 96 <210> SEQ ID NO 1 <211> LENGTH: 21 <212> TYPE: DNA <213> ORGANISM: ARTIFICIAL SEQUENCE <220> FEATURE: <223> OTHER INFORMATION: SYNTHETIC DNA <400> SEQUENCE: 1 tttgcacttg aactgtatgt a 21 <210> SEQ ID NO 2 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ARTIFICIAL SEQUENCE <220> FEATURE: <223> OTHER INFORMATION: SYNTHETIC DNA <400> SEQUENCE: 2 ggttcaatac ggcatttgga 20 <210> SEQ ID NO 3 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ARTIFICIAL SEQUENCE <220> FEATURE: <223> OTHER INFORMATION: SYNTHETIC DNA <400> SEQUENCE: 3 ggttcaatac ggtgtttgct 20 <210> SEQ ID NO 4 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ARTIFICIAL SEQUENCE <220> FEATURE: <223> OTHER INFORMATION: SYNTHETIC DNA <400> SEQUENCE: 4 tggcaaactt ccwccaaccc 20 <210> SEQ ID NO 5 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ARTIFICIAL SEQUENCE <220> FEATURE: <223> OTHER INFORMATION: SYNTHETIC DNA <400> SEQUENCE: 5 tggcaaacat cttcmaatcc 20 <210> SEQ ID NO 6 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: ARTIFICIAL SEQUENCE <220> FEATURE: <223> OTHER INFORMATION: SYNTHETIC DNA <400> SEQUENCE: 6 tggcagactt caachaaccc 20 <210> SEQ ID NO 7 <211> LENGTH: 783 <212> TYPE: DNA <213> ORGANISM: Coxsackievirus B <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(783) <223> OTHER INFORMATION: <400> SEQUENCE: 7 acg gaa gga agt gca cca cct cga atg tca ata cca ttc att agc ata 48 Thr Glu Gly Ser Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser Ile 1 5 10 15 ggc aat gca tac agc atg ttc tat gac ggg tgg gcc agg ttt gat aaa 96 Gly Asn Ala Tyr Ser Met Phe Tyr Asp Gly Trp Ala Arg Phe Asp Lys 20 25 30 caa ggg aca tat ggc atc aac aca cta aac aac atg ggg aca ctg tat 144 Gln Gly Thr Tyr Gly Ile Asn Thr Leu Asn Asn Met Gly Thr Leu Tyr 35 40 45 atg aga cat gtg aat gac ggt agc ccc ggt ccc atc gtg agt acg gtg 192 Met Arg His Val Asn Asp Gly Ser Pro Gly Pro Ile Val Ser Thr Val 50 55 60 cga att tac ttc aaa ccg aag cac gtt aag acg tgg gtc cca aga ccg 240 Arg Ile Tyr Phe Lys Pro Lys His Val Lys Thr Trp Val Pro Arg Pro 65 70 75 80 cct agg ttg tgt caa tat cag aag gca ggt aac gtg aat ttt gaa ccc 288 Pro Arg Leu Cys Gln Tyr Gln Lys Ala Gly Asn Val Asn Phe Glu Pro 85 90 95 act ggt gtg acc gag ggt agg aca gac ata acc acc atg caa act act 336 Thr Gly Val Thr Glu Gly Arg Thr Asp Ile Thr Thr Met Gln Thr Thr 100 105 110 ggc gcc ttc ggg cag cag tct ggt gct gta tac gtc ggc aac tac aga 384 Gly Ala Phe Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg 115 120 125 gtg gtg aat aga cat ctt gca aca cac atg gac tgg caa aat tgt gtt 432 Val Val Asn Arg His Leu Ala Thr His Met Asp Trp Gln Asn Cys Val 130 135 140 tgg gag gat tac aac aga gac ctt cta gtt agc acc acc aca gcc cac 480 Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His 145 150 155 160 ggg tgt gac acc ata gct aga tgc caa tgc acg aca gga gta tac ttc 528 Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe 165 170 175 tgt caa tcc aga aac aaa cat tat ccg gtg agt ttt gaa ggg cca ggg 576 Cys Gln Ser Arg Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro Gly 180 185 190 ctt gtg gaa gtt caa gag agt gag tat tac ccc aga agg ttc cag tcc 624 Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Arg Arg Phe Gln Ser 195 200 205 cat gta ctc cta gcc gcc gga ttc tcc gag ccc ggt gac tgc ggt ggc 672 His Val Leu Leu Ala Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly 210 215 220 atc tta agg tgt gat cac ggt gtc atc gga gtc gtg acc atg ggt ggt 720 Ile Leu Arg Cys Asp His Gly Val Ile Gly Val Val Thr Met Gly Gly 225 230 235 240 gaa ggg att gtc ggc ttt gct gat ata aga gac ctt cta tgg ttg gag 768 Glu Gly Ile Val Gly Phe Ala Asp Ile Arg Asp Leu Leu Trp Leu Glu 245 250 255 gac gat gcc atg gag 783 Asp Asp Ala Met Glu 260 <210> SEQ ID NO 8 <211> LENGTH: 261 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus B <400> SEQUENCE: 8 Thr Glu Gly Ser Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser Ile 1 5 10 15 Gly Asn Ala Tyr Ser Met Phe Tyr Asp Gly Trp Ala Arg Phe Asp Lys 20 25 30 Gln Gly Thr Tyr Gly Ile Asn Thr Leu Asn Asn Met Gly Thr Leu Tyr 35 40 45 Met Arg His Val Asn Asp Gly Ser Pro Gly Pro Ile Val Ser Thr Val 50 55 60 Arg Ile Tyr Phe Lys Pro Lys His Val Lys Thr Trp Val Pro Arg Pro 65 70 75 80 Pro Arg Leu Cys Gln Tyr Gln Lys Ala Gly Asn Val Asn Phe Glu Pro 85 90 95 Thr Gly Val Thr Glu Gly Arg Thr Asp Ile Thr Thr Met Gln Thr Thr 100 105 110 Gly Ala Phe Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg 115 120 125 Val Val Asn Arg His Leu Ala Thr His Met Asp Trp Gln Asn Cys Val 130 135 140 Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His 145 150 155 160 Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe 165 170 175 Cys Gln Ser Arg Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro Gly 180 185 190 Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Arg Arg Phe Gln Ser 195 200 205 His Val Leu Leu Ala Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly 210 215 220 Ile Leu Arg Cys Asp His Gly Val Ile Gly Val Val Thr Met Gly Gly 225 230 235 240 Glu Gly Ile Val Gly Phe Ala Asp Ile Arg Asp Leu Leu Trp Leu Glu 245 250 255 Asp Asp Ala Met Glu 260 <210> SEQ ID NO 9 <211> LENGTH: 633 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(633) <223> OTHER INFORMATION: <400> SEQUENCE: 9 gta ttt tgg act gaa gga aac gcg cct gcc aga atg tct ata cct ttc 48 Val Phe Trp Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe 1 5 10 15 att agc att ggc aac gca tac agt agt ttt tat gat gga tgg tca cac 96 Ile Ser Ile Gly Asn Ala Tyr Ser Ser Phe Tyr Asp Gly Trp Ser His 20 25 30 ttc acc caa aag gga atc tat gga tac aat aca ctg aat aag atg ggt 144 Phe Thr Gln Lys Gly Ile Tyr Gly Tyr Asn Thr Leu Asn Lys Met Gly 35 40 45 cag ctg ttt gtt aga cat gtg aac aaa gag act ccg aca ccg gtt acc 192 Gln Leu Phe Val Arg His Val Asn Lys Glu Thr Pro Thr Pro Val Thr 50 55 60 agt acc ata agg gtt tac ttc aag cca aag cac atc agg gct tgg gtc 240 Ser Thr Ile Arg Val Tyr Phe Lys Pro Lys His Ile Arg Ala Trp Val 65 70 75 80 cct aga ccc cca cga ttg tgc ccc tac gta aac aag aca aat gtg aat 288 Pro Arg Pro Pro Arg Leu Cys Pro Tyr Val Asn Lys Thr Asn Val Asn 85 90 95 ttc aac acc acg cag gtg aca aaa cca agg aac gat ctc aat gac gtg 336 Phe Asn Thr Thr Gln Val Thr Lys Pro Arg Asn Asp Leu Asn Asp Val 100 105 110 cca aag tcc gat cac aat gtg cgt acg tac ggg gcc ttt gga caa caa 384 Pro Lys Ser Asp His Asn Val Arg Thr Tyr Gly Ala Phe Gly Gln Gln 115 120 125 tcc ggc gcc gtg tac gtg ggc aac tac aga ctg gtg aat aga cac ctc 432 Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Leu Val Asn Arg His Leu 130 135 140 gcc aca cat aca gac tgg cag agt tgt gtt tgg gaa gat tat aac aga 480 Ala Thr His Thr Asp Trp Gln Ser Cys Val Trp Glu Asp Tyr Asn Arg 145 150 155 160 gac ctt ctc gtg tgt act acc aca gca cac gga tgt gac acc att gcc 528 Asp Leu Leu Val Cys Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala 165 170 175 aga tgc cag tgc aca act gga gtg tat ttc tgc caa tcc agg aat aag 576 Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Gln Ser Arg Asn Lys 180 185 190 cac tac ccg gtt agt ttt gaa gga ccg ggc ata gtg gaa gtt caa gaa 624 His Tyr Pro Val Ser Phe Glu Gly Pro Gly Ile Val Glu Val Gln Glu 195 200 205 agt gaa tat 633 Ser Glu Tyr 210 <210> SEQ ID NO 10 <211> LENGTH: 211 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 10 Val Phe Trp Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe 1 5 10 15 Ile Ser Ile Gly Asn Ala Tyr Ser Ser Phe Tyr Asp Gly Trp Ser His 20 25 30 Phe Thr Gln Lys Gly Ile Tyr Gly Tyr Asn Thr Leu Asn Lys Met Gly 35 40 45 Gln Leu Phe Val Arg His Val Asn Lys Glu Thr Pro Thr Pro Val Thr 50 55 60 Ser Thr Ile Arg Val Tyr Phe Lys Pro Lys His Ile Arg Ala Trp Val 65 70 75 80 Pro Arg Pro Pro Arg Leu Cys Pro Tyr Val Asn Lys Thr Asn Val Asn 85 90 95 Phe Asn Thr Thr Gln Val Thr Lys Pro Arg Asn Asp Leu Asn Asp Val 100 105 110 Pro Lys Ser Asp His Asn Val Arg Thr Tyr Gly Ala Phe Gly Gln Gln 115 120 125 Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Leu Val Asn Arg His Leu 130 135 140 Ala Thr His Thr Asp Trp Gln Ser Cys Val Trp Glu Asp Tyr Asn Arg 145 150 155 160 Asp Leu Leu Val Cys Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala 165 170 175 Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Gln Ser Arg Asn Lys 180 185 190 His Tyr Pro Val Ser Phe Glu Gly Pro Gly Ile Val Glu Val Gln Glu 195 200 205 Ser Glu Tyr 210 <210> SEQ ID NO 11 <211> LENGTH: 732 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(732) <223> OTHER INFORMATION: <400> SEQUENCE: 11 tgg act gag ggg aat gca cca cct aga atg tcc atc ccc ttc att agc 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 atc ggt aat gcc tac agt aat ttc tat gat ggt tgg tca cac ttt tcc 96 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 caa aat ggt gtg tac gga tac aac acg ctc aat cat atg ggc caa att 144 Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly Gln Ile 35 40 45 tac gtt aga cac ttg aac gga tcc tct cca cta ccc atg acg agt aca 192 Tyr Val Arg His Leu Asn Gly Ser Ser Pro Leu Pro Met Thr Ser Thr 50 55 60 gtt agg atg tac ttc aag cca aaa cat gtg aaa gtg tgg gtc ccg cgc 240 Val Arg Met Tyr Phe Lys Pro Lys His Val Lys Val Trp Val Pro Arg 65 70 75 80 ccg cct aga ttg tgc cag tac aaa aac gca tca act gtc aac ttc aca 288 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ser Thr Val Asn Phe Thr 85 90 95 ccc acc aac att act gaa aag agg aaa agc att aat tac ata cca gag 336 Pro Thr Asn Ile Thr Glu Lys Arg Lys Ser Ile Asn Tyr Ile Pro Glu 100 105 110 acg gtt aag ccg gat gtt tcg acg tac gga gcg ttc ggt cag caa tca 384 Thr Val Lys Pro Asp Val Ser Thr Tyr Gly Ala Phe Gly Gln Gln Ser 115 120 125 gga gca att tac gtg ggt aat tat agg gtg gtg aat aga cat cta gcc 432 Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 acc cac acc gac tgg caa aat tgt gtg tgg gat aac tac aac aga gat 480 Thr His Thr Asp Trp Gln Asn Cys Val Trp Asp Asn Tyr Asn Arg Asp 145 150 155 160 cta tta gtc agc act act act gcg cat ggg tgc gac acc atc gcc aga 528 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 tgc cag tgc aca acg ggc gtg tat ttt tgt gct tcg aaa aac aag cat 576 Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Lys Asn Lys His 180 185 190 tac ccg atc agc ttc gaa gga cca ggt ctg gtg gaa gta cag gaa agt 624 Tyr Pro Ile Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 gag tac tac cca cga agg tat cag tcc cac gtt tta ttg gca gca ggg 672 Glu Tyr Tyr Pro Arg Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly 210 215 220 ttt tca gag ccc gga gat tgt ggt gga atc ctg agg tgc gaa cat ggt 720 Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly 225 230 235 240 gtc att gga ctc 732 Val Ile Gly Leu <210> SEQ ID NO 12 <211> LENGTH: 244 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 12 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly Gln Ile 35 40 45 Tyr Val Arg His Leu Asn Gly Ser Ser Pro Leu Pro Met Thr Ser Thr 50 55 60 Val Arg Met Tyr Phe Lys Pro Lys His Val Lys Val Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ser Thr Val Asn Phe Thr 85 90 95 Pro Thr Asn Ile Thr Glu Lys Arg Lys Ser Ile Asn Tyr Ile Pro Glu 100 105 110 Thr Val Lys Pro Asp Val Ser Thr Tyr Gly Ala Phe Gly Gln Gln Ser 115 120 125 Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 Thr His Thr Asp Trp Gln Asn Cys Val Trp Asp Asn Tyr Asn Arg Asp 145 150 155 160 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Lys Asn Lys His 180 185 190 Tyr Pro Ile Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 Glu Tyr Tyr Pro Arg Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly 210 215 220 Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly 225 230 235 240 Val Ile Gly Leu <210> SEQ ID NO 13 <211> LENGTH: 663 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(663) <223> OTHER INFORMATION: <400> SEQUENCE: 13 tgg act gaa ggc aat gct cca cct agg atg tcc att ccg ttc att agt 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 ata ggc aat gcc tat agt agc ttt tat gat ggg tgg tca cat ttt gat 96 Ile Gly Asn Ala Tyr Ser Ser Phe Tyr Asp Gly Trp Ser His Phe Asp 20 25 30 agc aaa ggt gcc tac ggt ttc aac act ttg aac aaa atg gga cac atc 144 Ser Lys Gly Ala Tyr Gly Phe Asn Thr Leu Asn Lys Met Gly His Ile 35 40 45 tac tgc agg cac gtg aat aaa gag aca ccc gct gag gtc acc agt tat 192 Tyr Cys Arg His Val Asn Lys Glu Thr Pro Ala Glu Val Thr Ser Tyr 50 55 60 att aga att tat ttc aag ccc aag cac att aga gcg tgg gtt cca aga 240 Ile Arg Ile Tyr Phe Lys Pro Lys His Ile Arg Ala Trp Val Pro Arg 65 70 75 80 cca ccg cga ctt tgt cag tac aaa aat aag gca aac gtc aac ttt gac 288 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Lys Ala Asn Val Asn Phe Asp 85 90 95 gcc aca cca gtc act gat acg cgt gac acc atc aac act gtc ccc gta 336 Ala Thr Pro Val Thr Asp Thr Arg Asp Thr Ile Asn Thr Val Pro Val 100 105 110 tca agt cat ggg ggt gta cgc cgt ggt gat ttg gct gca ctg agc aca 384 Ser Ser His Gly Gly Val Arg Arg Gly Asp Leu Ala Ala Leu Ser Thr 115 120 125 cgt ggt gct ttt ggc caa caa tcc ggt gct ata tac gtg ggc aac tac 432 Arg Gly Ala Phe Gly Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn Tyr 130 135 140 agg gtg gtt aac agg cac tta gcc aca cgc act gat tgg cag aac tgc 480 Arg Val Val Asn Arg His Leu Ala Thr Arg Thr Asp Trp Gln Asn Cys 145 150 155 160 gtt tgg gaa gat tat aac agg gat ctt cta gtg agc aca aca acc gcc 528 Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala 165 170 175 cat gga tgc gac aca ata gcc aga tgc cag tgc aaa aca ggc gta tac 576 His Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys Lys Thr Gly Val Tyr 180 185 190 ttc tgc caa tcc aag ggc aag cac tac ccg gtt agt ttc gag ggt cca 624 Phe Cys Gln Ser Lys Gly Lys His Tyr Pro Val Ser Phe Glu Gly Pro 195 200 205 ggg ctg gta gag gtc cag aag agt gaa tac tac ccc agg 663 Gly Leu Val Glu Val Gln Lys Ser Glu Tyr Tyr Pro Arg 210 215 220 <210> SEQ ID NO 14 <211> LENGTH: 221 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 14 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Ser Phe Tyr Asp Gly Trp Ser His Phe Asp 20 25 30 Ser Lys Gly Ala Tyr Gly Phe Asn Thr Leu Asn Lys Met Gly His Ile 35 40 45 Tyr Cys Arg His Val Asn Lys Glu Thr Pro Ala Glu Val Thr Ser Tyr 50 55 60 Ile Arg Ile Tyr Phe Lys Pro Lys His Ile Arg Ala Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Lys Ala Asn Val Asn Phe Asp 85 90 95 Ala Thr Pro Val Thr Asp Thr Arg Asp Thr Ile Asn Thr Val Pro Val 100 105 110 Ser Ser His Gly Gly Val Arg Arg Gly Asp Leu Ala Ala Leu Ser Thr 115 120 125 Arg Gly Ala Phe Gly Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn Tyr 130 135 140 Arg Val Val Asn Arg His Leu Ala Thr Arg Thr Asp Trp Gln Asn Cys 145 150 155 160 Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala 165 170 175 His Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys Lys Thr Gly Val Tyr 180 185 190 Phe Cys Gln Ser Lys Gly Lys His Tyr Pro Val Ser Phe Glu Gly Pro 195 200 205 Gly Leu Val Glu Val Gln Lys Ser Glu Tyr Tyr Pro Arg 210 215 220 <210> SEQ ID NO 15 <211> LENGTH: 777 <212> TYPE: DNA <213> ORGANISM: Coxsackievirus B <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(777) <223> OTHER INFORMATION: <400> SEQUENCE: 15 act gag ggt aac gca cca cct cga gtg tct gtg ccc ttc atg agt att 48 Thr Glu Gly Asn Ala Pro Pro Arg Val Ser Val Pro Phe Met Ser Ile 1 5 10 15 gga aat gcc tat agc acc ttc tat gat ggc tgg tct gat ttt tca tca 96 Gly Asn Ala Tyr Ser Thr Phe Tyr Asp Gly Trp Ser Asp Phe Ser Ser 20 25 30 aag gga gtg tat ggc ttg aac act ctg aat aac atg gga aca ttg tat 144 Lys Gly Val Tyr Gly Leu Asn Thr Leu Asn Asn Met Gly Thr Leu Tyr 35 40 45 att cgg cac gtc aat ggg cct aac cca gtg cct atc acc agc acc gtg 192 Ile Arg His Val Asn Gly Pro Asn Pro Val Pro Ile Thr Ser Thr Val 50 55 60 cgt atc tat ttt aaa cca aag cat gtt aag gca tgg gtg cca cga cca 240 Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg Pro 65 70 75 80 cca aga tta tgc caa tac aac aca tca agg caa gtc aac ttc agt gtg 288 Pro Arg Leu Cys Gln Tyr Asn Thr Ser Arg Gln Val Asn Phe Ser Val 85 90 95 acg gga gta aca gaa tcc agg gag aat ata aca act atg aaa acc act 336 Thr Gly Val Thr Glu Ser Arg Glu Asn Ile Thr Thr Met Lys Thr Thr 100 105 110 ggg gct ttt ggt caa cag tct gga gca gcg tat gtg ggc aac tat agg 384 Gly Ala Phe Gly Gln Gln Ser Gly Ala Ala Tyr Val Gly Asn Tyr Arg 115 120 125 ata gtt aac aga cac ctg gca act cac acc gac tgg cag agt tgt gta 432 Ile Val Asn Arg His Leu Ala Thr His Thr Asp Trp Gln Ser Cys Val 130 135 140 tgg gaa gac tac aac agg gac ctt ctt gta agc acc acc aca gca cac 480 Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His 145 150 155 160 ggg tgt gac acc ata gca agg tgt cgt tgc aac tct ggt gtg tac ttc 528 Gly Cys Asp Thr Ile Ala Arg Cys Arg Cys Asn Ser Gly Val Tyr Phe 165 170 175 tgt gct tct aag aac aaa cac tac ccg gtg agt ttc gag ggg cca ggc 576 Cys Ala Ser Lys Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro Gly 180 185 190 ttg gtg gaa gtc cag gag agt gag tac tac cct aag aga tac cag tcc 624 Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser 195 200 205 cat gtt ctc cta gca gtc ggg ttc tcc gaa cca ggt gac tgt ggt ggc 672 His Val Leu Leu Ala Val Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly 210 215 220 atc tta agg tgt gaa cac ggt gtc atc ggc ctt gtc act atg ggt ggt 720 Ile Leu Arg Cys Glu His Gly Val Ile Gly Leu Val Thr Met Gly Gly 225 230 235 240 gaa agt gtt gtg ggg ttc aca gat atc cgc gac ctg ctg tgg att gag 768 Glu Ser Val Val Gly Phe Thr Asp Ile Arg Asp Leu Leu Trp Ile Glu 245 250 255 gac gac gct 777 Asp Asp Ala <210> SEQ ID NO 16 <211> LENGTH: 259 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus B <400> SEQUENCE: 16 Thr Glu Gly Asn Ala Pro Pro Arg Val Ser Val Pro Phe Met Ser Ile 1 5 10 15 Gly Asn Ala Tyr Ser Thr Phe Tyr Asp Gly Trp Ser Asp Phe Ser Ser 20 25 30 Lys Gly Val Tyr Gly Leu Asn Thr Leu Asn Asn Met Gly Thr Leu Tyr 35 40 45 Ile Arg His Val Asn Gly Pro Asn Pro Val Pro Ile Thr Ser Thr Val 50 55 60 Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg Pro 65 70 75 80 Pro Arg Leu Cys Gln Tyr Asn Thr Ser Arg Gln Val Asn Phe Ser Val 85 90 95 Thr Gly Val Thr Glu Ser Arg Glu Asn Ile Thr Thr Met Lys Thr Thr 100 105 110 Gly Ala Phe Gly Gln Gln Ser Gly Ala Ala Tyr Val Gly Asn Tyr Arg 115 120 125 Ile Val Asn Arg His Leu Ala Thr His Thr Asp Trp Gln Ser Cys Val 130 135 140 Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His 145 150 155 160 Gly Cys Asp Thr Ile Ala Arg Cys Arg Cys Asn Ser Gly Val Tyr Phe 165 170 175 Cys Ala Ser Lys Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro Gly 180 185 190 Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser 195 200 205 His Val Leu Leu Ala Val Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly 210 215 220 Ile Leu Arg Cys Glu His Gly Val Ile Gly Leu Val Thr Met Gly Gly 225 230 235 240 Glu Ser Val Val Gly Phe Thr Asp Ile Arg Asp Leu Leu Trp Ile Glu 245 250 255 Asp Asp Ala <210> SEQ ID NO 17 <211> LENGTH: 744 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(744) <223> OTHER INFORMATION: <400> SEQUENCE: 17 tgg acg gag ggc aac gca cct gcg aga atg tcc atc cca ttc atc agc 48 Trp Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 att ggt aat gcg tac agc aat ttt tat gat ggg tgg tca cac ttt aca 96 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Thr 20 25 30 caa gat ggg gtg tat ggg ttc aac tct ctc aat aac atg ggc tct ata 144 Gln Asp Gly Val Tyr Gly Phe Asn Ser Leu Asn Asn Met Gly Ser Ile 35 40 45 tac att aga cat gtc aac gag cag agt ccc cat gct atc acg agc act 192 Tyr Ile Arg His Val Asn Glu Gln Ser Pro His Ala Ile Thr Ser Thr 50 55 60 gtg aga gtg tac ttc aaa ccc aaa cac gtg agg gca tgg gtc cca agg 240 Val Arg Val Tyr Phe Lys Pro Lys His Val Arg Ala Trp Val Pro Arg 65 70 75 80 cca cca agg tta tgt gca tat gaa aaa tct agc aac gta aac ttc aaa 288 Pro Pro Arg Leu Cys Ala Tyr Glu Lys Ser Ser Asn Val Asn Phe Lys 85 90 95 ccc aca gat gtg acc act acg cgc tca tcc atc acg gaa gtc ccc agt 336 Pro Thr Asp Val Thr Thr Thr Arg Ser Ser Ile Thr Glu Val Pro Ser 100 105 110 ctt agg cca tcg gta caa aac acc ggg gct ttc gga caa caa tcc gga 384 Leu Arg Pro Ser Val Gln Asn Thr Gly Ala Phe Gly Gln Gln Ser Gly 115 120 125 gca gtt tac gtt gga aac tat aga gtt gtt aac agg cac tta gcc acc 432 Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala Thr 130 135 140 cac aat gat tgg caa aat tgc gtg tgg gaa gat tac aat aga gat ctt 480 His Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu 145 150 155 160 ctt ata agc aca aca act gct cac ggg tgt gac acc ata gcc aga tgc 528 Leu Ile Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys 165 170 175 cgg tgc aca aca gga gtg tat ttc tgt caa tcc aaa aac aaa cac tac 576 Arg Cys Thr Thr Gly Val Tyr Phe Cys Gln Ser Lys Asn Lys His Tyr 180 185 190 cca gtt agt ttt gaa ggg cca ggg tta gtg gaa gtt caa gag agt gag 624 Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu 195 200 205 tac tat cct aaa agg tat caa tcc cac gtg cta ctt gct gca ggt ttc 672 Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly Phe 210 215 220 tct gag cca ggc gac tgt ggt gga atc ttg agg tgt gaa cat ggc gtc 720 Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly Val 225 230 235 240 atc ggt att gtg acc atg ggt ggt 744 Ile Gly Ile Val Thr Met Gly Gly 245 <210> SEQ ID NO 18 <211> LENGTH: 248 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 18 Trp Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Thr 20 25 30 Gln Asp Gly Val Tyr Gly Phe Asn Ser Leu Asn Asn Met Gly Ser Ile 35 40 45 Tyr Ile Arg His Val Asn Glu Gln Ser Pro His Ala Ile Thr Ser Thr 50 55 60 Val Arg Val Tyr Phe Lys Pro Lys His Val Arg Ala Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Ala Tyr Glu Lys Ser Ser Asn Val Asn Phe Lys 85 90 95 Pro Thr Asp Val Thr Thr Thr Arg Ser Ser Ile Thr Glu Val Pro Ser 100 105 110 Leu Arg Pro Ser Val Gln Asn Thr Gly Ala Phe Gly Gln Gln Ser Gly 115 120 125 Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala Thr 130 135 140 His Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu 145 150 155 160 Leu Ile Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys 165 170 175 Arg Cys Thr Thr Gly Val Tyr Phe Cys Gln Ser Lys Asn Lys His Tyr 180 185 190 Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu 195 200 205 Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly Phe 210 215 220 Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly Val 225 230 235 240 Ile Gly Ile Val Thr Met Gly Gly 245 <210> SEQ ID NO 19 <211> LENGTH: 654 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(654) <223> OTHER INFORMATION: <400> SEQUENCE: 19 act gag ggg aat gca cca gct cgc atg tca att cca ttc atc agc ata 48 Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe Ile Ser Ile 1 5 10 15 ggc aat gct tac agt aac ttc tat gat gga tgg tca cac ttt tca caa 96 Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser Gln 20 25 30 acc ggt gtc tat ggt ttt act aca ctg aat aat atg ggt caa ctg ttc 144 Thr Gly Val Tyr Gly Phe Thr Thr Leu Asn Asn Met Gly Gln Leu Phe 35 40 45 ttc aga cac gtt aac aag cct aac ccg gct gcc att acc agt gta gca 192 Phe Arg His Val Asn Lys Pro Asn Pro Ala Ala Ile Thr Ser Val Ala 50 55 60 cgc ata tat ttc aag cca aaa cac gtg cgt gct tgg gtg ccc agg cca 240 Arg Ile Tyr Phe Lys Pro Lys His Val Arg Ala Trp Val Pro Arg Pro 65 70 75 80 cca cgc ctg tgc ccg tat atc aat agc acc aat gtc aat ttc aaa cct 288 Pro Arg Leu Cys Pro Tyr Ile Asn Ser Thr Asn Val Asn Phe Lys Pro 85 90 95 aag cca gtg aca aag gtg cgg aat aac ata ata aca aca gga gca ttt 336 Lys Pro Val Thr Lys Val Arg Asn Asn Ile Ile Thr Thr Gly Ala Phe 100 105 110 gga cag cag tct ggt gca gtt tac gta gga aac tac agg gta gtg aac 384 Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn 115 120 125 agg cat ctc gct aca cat aat gat tgg cag aac tgt gtg tgg gaa gat 432 Arg His Leu Ala Thr His Asn Asp Trp Gln Asn Cys Val Trp Glu Asp 130 135 140 tac agt aga gac ctt cta gtg agc acc acc acc gcc cac ggg tgc gac 480 Tyr Ser Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp 145 150 155 160 act ata gcc aga tgc caa tgt acg aca ggt gta tac ttc tgt gcc tcg 528 Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser 165 170 175 aag aac aag cac tac cat att act ttt gag ggg cca ggt cta gtg gaa 576 Lys Asn Lys His Tyr His Ile Thr Phe Glu Gly Pro Gly Leu Val Glu 180 185 190 gtt caa gag agt gaa tac tac ccc aag agg ttt caa tca cac gtc tta 624 Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Phe Gln Ser His Val Leu 195 200 205 cta gct aca ggg ttc tcg gaa cca gga gac 654 Leu Ala Thr Gly Phe Ser Glu Pro Gly Asp 210 215 <210> SEQ ID NO 20 <211> LENGTH: 218 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 20 Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe Ile Ser Ile 1 5 10 15 Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser Gln 20 25 30 Thr Gly Val Tyr Gly Phe Thr Thr Leu Asn Asn Met Gly Gln Leu Phe 35 40 45 Phe Arg His Val Asn Lys Pro Asn Pro Ala Ala Ile Thr Ser Val Ala 50 55 60 Arg Ile Tyr Phe Lys Pro Lys His Val Arg Ala Trp Val Pro Arg Pro 65 70 75 80 Pro Arg Leu Cys Pro Tyr Ile Asn Ser Thr Asn Val Asn Phe Lys Pro 85 90 95 Lys Pro Val Thr Lys Val Arg Asn Asn Ile Ile Thr Thr Gly Ala Phe 100 105 110 Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn 115 120 125 Arg His Leu Ala Thr His Asn Asp Trp Gln Asn Cys Val Trp Glu Asp 130 135 140 Tyr Ser Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp 145 150 155 160 Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser 165 170 175 Lys Asn Lys His Tyr His Ile Thr Phe Glu Gly Pro Gly Leu Val Glu 180 185 190 Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Phe Gln Ser His Val Leu 195 200 205 Leu Ala Thr Gly Phe Ser Glu Pro Gly Asp 210 215 <210> SEQ ID NO 21 <211> LENGTH: 777 <212> TYPE: DNA <213> ORGANISM: Coxsackievirus B <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(777) <223> OTHER INFORMATION: <400> SEQUENCE: 21 acg gag ggg aat gct cca cca agg atg tca att cca ttt att agc att 48 Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser Ile 1 5 10 15 ggc aat gcg tat agc tgt ttt tat gat ggg tgg aca cag ttc tca aga 96 Gly Asn Ala Tyr Ser Cys Phe Tyr Asp Gly Trp Thr Gln Phe Ser Arg 20 25 30 aac ggg gtc tat ggg att aac act cta aac aac atg ggc act tta tac 144 Asn Gly Val Tyr Gly Ile Asn Thr Leu Asn Asn Met Gly Thr Leu Tyr 35 40 45 atg cgg cat gtc aac gaa gca gga cag ggc cca att aag agt act gtt 192 Met Arg His Val Asn Glu Ala Gly Gln Gly Pro Ile Lys Ser Thr Val 50 55 60 aga ata tat ttc aaa cct aag cat gtg aag gcg tgg gtg ccc cgc ccg 240 Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg Pro 65 70 75 80 cct agg ctg tgt cag tat gag aaa caa aag aat gtc aat ttt aaa ccc 288 Pro Arg Leu Cys Gln Tyr Glu Lys Gln Lys Asn Val Asn Phe Lys Pro 85 90 95 aca gga gta acc aca cat agg ctg gac att gta aca aca ggc gct ttt 336 Thr Gly Val Thr Thr His Arg Leu Asp Ile Val Thr Thr Gly Ala Phe 100 105 110 ggt cag caa tct ggc gca ata tac gta gga aac tac aga gtg gtt aac 384 Gly Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn 115 120 125 aga cac tta gcc acg cac aaa gat tgg gag agc tgc gtg tgg gaa gac 432 Arg His Leu Ala Thr His Lys Asp Trp Glu Ser Cys Val Trp Glu Asp 130 135 140 tac aac aga gac ctc cta gtg agc acc acc acc gcc cac ggg tgt gac 480 Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp 145 150 155 160 acc ata gct aga tgc caa tgt acg aca ggt gtg tac ttc tgt gcc tcc 528 Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser 165 170 175 aag aac aag cac tac ccc gtc gct ttt gag ggg cca ggt cta gtg gaa 576 Lys Asn Lys His Tyr Pro Val Ala Phe Glu Gly Pro Gly Leu Val Glu 180 185 190 gtt cag gaa agc gaa tac tac cca aag aga tat cag tcc cat gtg ttg 624 Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu 195 200 205 ctc gct gcg gga ttt tca gag cca ggc gat tgt gga ggt atc ctg agg 672 Leu Ala Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg 210 215 220 tgc gag cac gga gtc att gga ctt gtg acc atg ggg ggt gaa ggc atc 720 Cys Glu His Gly Val Ile Gly Leu Val Thr Met Gly Gly Glu Gly Ile 225 230 235 240 gtc ggc ttt gct gat gtg cgc gac atc cta tgg ttg gaa gat gac gcc 768 Val Gly Phe Ala Asp Val Arg Asp Ile Leu Trp Leu Glu Asp Asp Ala 245 250 255 atg gaa cag 777 Met Glu Gln <210> SEQ ID NO 22 <211> LENGTH: 259 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus B <400> SEQUENCE: 22 Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser Ile 1 5 10 15 Gly Asn Ala Tyr Ser Cys Phe Tyr Asp Gly Trp Thr Gln Phe Ser Arg 20 25 30 Asn Gly Val Tyr Gly Ile Asn Thr Leu Asn Asn Met Gly Thr Leu Tyr 35 40 45 Met Arg His Val Asn Glu Ala Gly Gln Gly Pro Ile Lys Ser Thr Val 50 55 60 Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg Pro 65 70 75 80 Pro Arg Leu Cys Gln Tyr Glu Lys Gln Lys Asn Val Asn Phe Lys Pro 85 90 95 Thr Gly Val Thr Thr His Arg Leu Asp Ile Val Thr Thr Gly Ala Phe 100 105 110 Gly Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn 115 120 125 Arg His Leu Ala Thr His Lys Asp Trp Glu Ser Cys Val Trp Glu Asp 130 135 140 Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp 145 150 155 160 Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser 165 170 175 Lys Asn Lys His Tyr Pro Val Ala Phe Glu Gly Pro Gly Leu Val Glu 180 185 190 Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu 195 200 205 Leu Ala Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg 210 215 220 Cys Glu His Gly Val Ile Gly Leu Val Thr Met Gly Gly Glu Gly Ile 225 230 235 240 Val Gly Phe Ala Asp Val Arg Asp Ile Leu Trp Leu Glu Asp Asp Ala 245 250 255 Met Glu Gln <210> SEQ ID NO 23 <211> LENGTH: 735 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(735) <223> OTHER INFORMATION: <400> SEQUENCE: 23 agc ata ttc tgg aca gag ggg aat gca cct gct agg atg tcc ata ccc 48 Ser Ile Phe Trp Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro 1 5 10 15 ttc att agc atc gga aat gca tac agt aac ttc tat gac ggg tgg tca 96 Phe Ile Ser Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser 20 25 30 cac ttc tca cag aac ggg gtt tac ggt ttc acc act ttg aac aac atg 144 His Phe Ser Gln Asn Gly Val Tyr Gly Phe Thr Thr Leu Asn Asn Met 35 40 45 ggc cag cta ttc ttc aga cac gtt aac aag ccc aat ccg gca aca atc 192 Gly Gln Leu Phe Phe Arg His Val Asn Lys Pro Asn Pro Ala Thr Ile 50 55 60 acc agc gta gct cgc att tac ttc aag ccc aaa cac gtg aga gcc tgg 240 Thr Ser Val Ala Arg Ile Tyr Phe Lys Pro Lys His Val Arg Ala Trp 65 70 75 80 gtg cct aga cca cct cgg ttg tgc ccc tat atc aat agt ggc aat gta 288 Val Pro Arg Pro Pro Arg Leu Cys Pro Tyr Ile Asn Ser Gly Asn Val 85 90 95 aat ttt gac cca aaa ccc gtg acg gaa gtc aga agc agc atc atc acc 336 Asn Phe Asp Pro Lys Pro Val Thr Glu Val Arg Ser Ser Ile Ile Thr 100 105 110 acc ggg gcc ttc ggg caa cag tca ggg gcg gtt tac gtg gga aat tac 384 Thr Gly Ala Phe Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr 115 120 125 agg gtg gtg aat aga cac ctc gcc acg cgc aat gat tgg tta agt tgc 432 Arg Val Val Asn Arg His Leu Ala Thr Arg Asn Asp Trp Leu Ser Cys 130 135 140 gtg tgg gaa gat tac aac aga gac ctc cta gtg agc acc acc aca gcg 480 Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala 145 150 155 160 cat ggc tgc gac act ata gcc agg tgc cgt tgc acc acg ggt gta tac 528 His Gly Cys Asp Thr Ile Ala Arg Cys Arg Cys Thr Thr Gly Val Tyr 165 170 175 ttt tgc gcg tcc aaa aac aaa cac tac ccg gtc tcc ttt gaa ggg cca 576 Phe Cys Ala Ser Lys Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro 180 185 190 ggc tta gtg gaa gtc cag gag agt gaa tac tat ccc aaa aga tat caa 624 Gly Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln 195 200 205 tcc cat gtt ctt tta gcg gcc ggg ttc tcc gac ccg ggc gat tgt ggt 672 Ser His Val Leu Leu Ala Ala Gly Phe Ser Asp Pro Gly Asp Cys Gly 210 215 220 ggt atc ttg agg tgc gag cac ggt gtc atc ggt ctc gtt acc atg ggt 720 Gly Ile Leu Arg Cys Glu His Gly Val Ile Gly Leu Val Thr Met Gly 225 230 235 240 ggt gaa ggc gtt gta 735 Gly Glu Gly Val Val 245 <210> SEQ ID NO 24 <211> LENGTH: 245 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 24 Ser Ile Phe Trp Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro 1 5 10 15 Phe Ile Ser Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser 20 25 30 His Phe Ser Gln Asn Gly Val Tyr Gly Phe Thr Thr Leu Asn Asn Met 35 40 45 Gly Gln Leu Phe Phe Arg His Val Asn Lys Pro Asn Pro Ala Thr Ile 50 55 60 Thr Ser Val Ala Arg Ile Tyr Phe Lys Pro Lys His Val Arg Ala Trp 65 70 75 80 Val Pro Arg Pro Pro Arg Leu Cys Pro Tyr Ile Asn Ser Gly Asn Val 85 90 95 Asn Phe Asp Pro Lys Pro Val Thr Glu Val Arg Ser Ser Ile Ile Thr 100 105 110 Thr Gly Ala Phe Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr 115 120 125 Arg Val Val Asn Arg His Leu Ala Thr Arg Asn Asp Trp Leu Ser Cys 130 135 140 Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala 145 150 155 160 His Gly Cys Asp Thr Ile Ala Arg Cys Arg Cys Thr Thr Gly Val Tyr 165 170 175 Phe Cys Ala Ser Lys Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro 180 185 190 Gly Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln 195 200 205 Ser His Val Leu Leu Ala Ala Gly Phe Ser Asp Pro Gly Asp Cys Gly 210 215 220 Gly Ile Leu Arg Cys Glu His Gly Val Ile Gly Leu Val Thr Met Gly 225 230 235 240 Gly Glu Gly Val Val 245 <210> SEQ ID NO 25 <211> LENGTH: 720 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(720) <223> OTHER INFORMATION: <400> SEQUENCE: 25 tgg att gag gga aac gcc ccc ccc aga atg tcc atc cct ttt atg agt 48 Trp Ile Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 gtg gga aac gca tat agc aac ttc tac gat gga tgg tct cac ttt tcg 96 Val Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 caa aca ggt gtg tac ggt ttc aac act ctc aac aac atg ggg aag tta 144 Gln Thr Gly Val Tyr Gly Phe Asn Thr Leu Asn Asn Met Gly Lys Leu 35 40 45 tat ttc aga cat gtg aac gac aag aca att agc cca atc aca agc aaa 192 Tyr Phe Arg His Val Asn Asp Lys Thr Ile Ser Pro Ile Thr Ser Lys 50 55 60 gtc cgc atc tac ttc aaa cca aaa cac gtg aaa gct tgg gta ccc cgc 240 Val Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 cca cct aga ctg tgt gag tac acg cac aaa gac aat gtg gat ttt gaa 288 Pro Pro Arg Leu Cys Glu Tyr Thr His Lys Asp Asn Val Asp Phe Glu 85 90 95 ccc aaa ggc gtt acc aca tcc cgc acc caa cta aca att agc aac tcc 336 Pro Lys Gly Val Thr Thr Ser Arg Thr Gln Leu Thr Ile Ser Asn Ser 100 105 110 aca cac atg gaa aat tat ggt gcc ttt gga caa caa tct gga gcc gtc 384 Thr His Met Glu Asn Tyr Gly Ala Phe Gly Gln Gln Ser Gly Ala Val 115 120 125 tac gtg ggc aat tat aga cta gta aac aga cac cta gcc acg cac aat 432 Tyr Val Gly Asn Tyr Arg Leu Val Asn Arg His Leu Ala Thr His Asn 130 135 140 gat tgg cag aac tgt gta tgg gaa gac tac aat aga gac ctt tta gtt 480 Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val 145 150 155 160 agc acc acc aca gcc cat gga tgt gac act att gct agg tgc caa tgc 528 Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys 165 170 175 acc act ggt gta tac ttt tgt gct tcc aaa aac aag cac tat ccc gtg 576 Thr Thr Gly Val Tyr Phe Cys Ala Ser Lys Asn Lys His Tyr Pro Val 180 185 190 gca ttt gag gga cct ggt cta gtg gaa gtg cag gag agc gag tat tac 624 Ala Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr 195 200 205 cct aaa aga tat cag tct cat gtt ctt ctt gcc gca ggg ttc tcc gag 672 Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly Phe Ser Glu 210 215 220 cca ggg gat tgt ggt ggc atc ctt agg tgt gaa cat ggt gtc ata ggc 720 Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly Val Ile Gly 225 230 235 240 <210> SEQ ID NO 26 <211> LENGTH: 240 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 26 Trp Ile Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 Val Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Thr Gly Val Tyr Gly Phe Asn Thr Leu Asn Asn Met Gly Lys Leu 35 40 45 Tyr Phe Arg His Val Asn Asp Lys Thr Ile Ser Pro Ile Thr Ser Lys 50 55 60 Val Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Glu Tyr Thr His Lys Asp Asn Val Asp Phe Glu 85 90 95 Pro Lys Gly Val Thr Thr Ser Arg Thr Gln Leu Thr Ile Ser Asn Ser 100 105 110 Thr His Met Glu Asn Tyr Gly Ala Phe Gly Gln Gln Ser Gly Ala Val 115 120 125 Tyr Val Gly Asn Tyr Arg Leu Val Asn Arg His Leu Ala Thr His Asn 130 135 140 Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val 145 150 155 160 Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys 165 170 175 Thr Thr Gly Val Tyr Phe Cys Ala Ser Lys Asn Lys His Tyr Pro Val 180 185 190 Ala Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr 195 200 205 Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly Phe Ser Glu 210 215 220 Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly Val Ile Gly 225 230 235 240 <210> SEQ ID NO 27 <211> LENGTH: 621 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(621) <223> OTHER INFORMATION: <400> SEQUENCE: 27 gtg ttc tgg acc gaa ggt aat gca cca gca cga atg tcc ata cca ttt 48 Val Phe Trp Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe 1 5 10 15 att agc atc ggg aat gct tat agt aat ttc tat gat ggg tgg tcc cat 96 Ile Ser Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His 20 25 30 ttt aca caa gat ggt ggc tat ggg tac aac acg ctt aac aaa atg ggc 144 Phe Thr Gln Asp Gly Gly Tyr Gly Tyr Asn Thr Leu Asn Lys Met Gly 35 40 45 aaa att tat gtc agg cat gtg aac aaa caa acc ccc act gac gtg act 192 Lys Ile Tyr Val Arg His Val Asn Lys Gln Thr Pro Thr Asp Val Thr 50 55 60 agc act att aga att tac ttt aaa ccc aaa cat gtg cgg gct tgg ata 240 Ser Thr Ile Arg Ile Tyr Phe Lys Pro Lys His Val Arg Ala Trp Ile 65 70 75 80 cca cgt cca cct aga ctg tgt caa tac aag aat aaa gca aac gtg aat 288 Pro Arg Pro Pro Arg Leu Cys Gln Tyr Lys Asn Lys Ala Asn Val Asn 85 90 95 ttt gaa gtt act agc gtt aca act gct aga act aac cta aat gat gtc 336 Phe Glu Val Thr Ser Val Thr Thr Ala Arg Thr Asn Leu Asn Asp Val 100 105 110 ccg aca ccc agc cac agt agc agt gta cat ttg cgc cta agc act caa 384 Pro Thr Pro Ser His Ser Ser Ser Val His Leu Arg Leu Ser Thr Gln 115 120 125 ggg gcg tct ggg cat caa gca ggt gct gca tac gtt ggc aat tac cgg 432 Gly Ala Ser Gly His Gln Ala Gly Ala Ala Tyr Val Gly Asn Tyr Arg 130 135 140 gtg gtt aac aga cac cta gct acg caa cag gat tgg caa aac tgt gta 480 Val Val Asn Arg His Leu Ala Thr Gln Gln Asp Trp Gln Asn Cys Val 145 150 155 160 tgg gaa gac tac aac aga gac ctc tta gtg agc act acg aca gca cat 528 Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His 165 170 175 gga tgt gac acc att gct aga tgc cag tgt act aca ggt gtg tac ttc 576 Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe 180 185 190 tgt gct tcg aaa aac aag cat tac cca gtg agt ttt gaa ggt cca 621 Cys Ala Ser Lys Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro 195 200 205 <210> SEQ ID NO 28 <211> LENGTH: 207 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 28 Val Phe Trp Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe 1 5 10 15 Ile Ser Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His 20 25 30 Phe Thr Gln Asp Gly Gly Tyr Gly Tyr Asn Thr Leu Asn Lys Met Gly 35 40 45 Lys Ile Tyr Val Arg His Val Asn Lys Gln Thr Pro Thr Asp Val Thr 50 55 60 Ser Thr Ile Arg Ile Tyr Phe Lys Pro Lys His Val Arg Ala Trp Ile 65 70 75 80 Pro Arg Pro Pro Arg Leu Cys Gln Tyr Lys Asn Lys Ala Asn Val Asn 85 90 95 Phe Glu Val Thr Ser Val Thr Thr Ala Arg Thr Asn Leu Asn Asp Val 100 105 110 Pro Thr Pro Ser His Ser Ser Ser Val His Leu Arg Leu Ser Thr Gln 115 120 125 Gly Ala Ser Gly His Gln Ala Gly Ala Ala Tyr Val Gly Asn Tyr Arg 130 135 140 Val Val Asn Arg His Leu Ala Thr Gln Gln Asp Trp Gln Asn Cys Val 145 150 155 160 Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His 165 170 175 Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe 180 185 190 Cys Ala Ser Lys Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro 195 200 205 <210> SEQ ID NO 29 <211> LENGTH: 645 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(645) <223> OTHER INFORMATION: <400> SEQUENCE: 29 tgg att gag gga aac gcc ccc ccc aga atg tcc atc cct ttt atg agt 48 Trp Ile Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 gtg gga aac gca tat agc aac ttc tac gat gga tgg tct cac ttt tcg 96 Val Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 caa aca ggt gtg tac ggt ttc aac act ctc aac aac atg ggg aag tta 144 Gln Thr Gly Val Tyr Gly Phe Asn Thr Leu Asn Asn Met Gly Lys Leu 35 40 45 tat ttc aga cat gtg aac gac aag aca att agc cca atc aca agc aaa 192 Tyr Phe Arg His Val Asn Asp Lys Thr Ile Ser Pro Ile Thr Ser Lys 50 55 60 gtc cgc atc tac ttc aaa cca aaa cac gtg aaa gct tgg gta ccc cgc 240 Val Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 cca cct aga ctg tgt gag tac acg cac aaa gac aat gtg gat ttt gaa 288 Pro Pro Arg Leu Cys Glu Tyr Thr His Lys Asp Asn Val Asp Phe Glu 85 90 95 ccc aaa ggc gtt acc aca tcc cgc acc caa cta aca att agc aac tcc 336 Pro Lys Gly Val Thr Thr Ser Arg Thr Gln Leu Thr Ile Ser Asn Ser 100 105 110 aca cac atg gaa aat tat ggt gcc ttt gga caa caa tct gga gcc gtc 384 Thr His Met Glu Asn Tyr Gly Ala Phe Gly Gln Gln Ser Gly Ala Val 115 120 125 tac gtg ggc aat tat aga cta gta aac aga cac cta gcc acg cac aat 432 Tyr Val Gly Asn Tyr Arg Leu Val Asn Arg His Leu Ala Thr His Asn 130 135 140 gat tgg cag aac tgt gta tgg gaa gac tac aat aga gac ctt tta gtt 480 Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val 145 150 155 160 agc acc acc aca gcc cat gga tgt gac act att gct agg tgc caa tgc 528 Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys 165 170 175 acc act ggt gta tac ttt tgt gct tcc aaa aac aag cac tat ccc gtg 576 Thr Thr Gly Val Tyr Phe Cys Ala Ser Lys Asn Lys His Tyr Pro Val 180 185 190 gca ttt gag gga cct ggt cta gtg gaa gtg cag gag agc gag tat tac 624 Ala Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr 195 200 205 cct aaa aga tat cag tct cat 645 Pro Lys Arg Tyr Gln Ser His 210 215 <210> SEQ ID NO 30 <211> LENGTH: 215 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 30 Trp Ile Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 Val Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Thr Gly Val Tyr Gly Phe Asn Thr Leu Asn Asn Met Gly Lys Leu 35 40 45 Tyr Phe Arg His Val Asn Asp Lys Thr Ile Ser Pro Ile Thr Ser Lys 50 55 60 Val Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Glu Tyr Thr His Lys Asp Asn Val Asp Phe Glu 85 90 95 Pro Lys Gly Val Thr Thr Ser Arg Thr Gln Leu Thr Ile Ser Asn Ser 100 105 110 Thr His Met Glu Asn Tyr Gly Ala Phe Gly Gln Gln Ser Gly Ala Val 115 120 125 Tyr Val Gly Asn Tyr Arg Leu Val Asn Arg His Leu Ala Thr His Asn 130 135 140 Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val 145 150 155 160 Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys 165 170 175 Thr Thr Gly Val Tyr Phe Cys Ala Ser Lys Asn Lys His Tyr Pro Val 180 185 190 Ala Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr 195 200 205 Pro Lys Arg Tyr Gln Ser His 210 215 <210> SEQ ID NO 31 <211> LENGTH: 750 <212> TYPE: DNA <213> ORGANISM: Coxsackievirus A <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(750) <223> OTHER INFORMATION: <400> SEQUENCE: 31 tgg aca gaa gga aat gcg cca gcg cga atg tca atc ccc ttc atc agc 48 Trp Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 ata ggg aat gct tac agc aac ttc tat gac gga tgg tca aac ttc gac 96 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser Asn Phe Asp 20 25 30 cag aag ggc tca tat ggg tac aac aca cta aac aac cta ggt cac ata 144 Gln Lys Gly Ser Tyr Gly Tyr Asn Thr Leu Asn Asn Leu Gly His Ile 35 40 45 tat gtg agg cat gtg agc ggt agt agc cct cat ccc atc acg agt acc 192 Tyr Val Arg His Val Ser Gly Ser Ser Pro His Pro Ile Thr Ser Thr 50 55 60 atc aga ata tac ttt aaa cca aag cac act aga gcc tgg gtg ccg cgg 240 Ile Arg Ile Tyr Phe Lys Pro Lys His Thr Arg Ala Trp Val Pro Arg 65 70 75 80 cct cct cga ctg tgt cag tac aag aaa gca ttc agt gtg gat ttt aca 288 Pro Pro Arg Leu Cys Gln Tyr Lys Lys Ala Phe Ser Val Asp Phe Thr 85 90 95 cca acg ccc atc act gac act agg aaa gac atc aac acc gta aca tca 336 Pro Thr Pro Ile Thr Asp Thr Arg Lys Asp Ile Asn Thr Val Thr Ser 100 105 110 ata caa agt ggg cgt cga ggc gac atg tct gca cta aac acc cac gga 384 Ile Gln Ser Gly Arg Arg Gly Asp Met Ser Ala Leu Asn Thr His Gly 115 120 125 gcg ttt gga cag caa tct gga gca gtg tat gtg gga aat tat aga gta 432 Ala Phe Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Val 130 135 140 gtg aac aga cat ctc gcc aca cac aac gat tgg caa agg tgt gtg tgg 480 Val Asn Arg His Leu Ala Thr His Asn Asp Trp Gln Arg Cys Val Trp 145 150 155 160 gaa aac tat aat agg gac ctc ctg gtc agc acc acc aca gct cac gga 528 Glu Asn Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly 165 170 175 tgt gac acc atc gct aga tgt caa tgc act act gga ata tac tac tgt 576 Cys Asp Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Ile Tyr Tyr Cys 180 185 190 gcc tcc aag aat aaa cac tac ccc gta gct ttt gag gga ccc ggt ctg 624 Ala Ser Lys Asn Lys His Tyr Pro Val Ala Phe Glu Gly Pro Gly Leu 195 200 205 gtg gag gtg cag gag aat gaa tac tac ccc aag aga tac caa tct cat 672 Val Glu Val Gln Glu Asn Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His 210 215 220 gtt ctt ctc gcc gcg ggg ttc tct gag cca ggg gat tgt ggc ggc att 720 Val Leu Leu Ala Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile 225 230 235 240 ctt agg tgt gaa cat ggc gtc ata ggc att 750 Leu Arg Cys Glu His Gly Val Ile Gly Ile 245 250 <210> SEQ ID NO 32 <211> LENGTH: 250 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus A <400> SEQUENCE: 32 Trp Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser Asn Phe Asp 20 25 30 Gln Lys Gly Ser Tyr Gly Tyr Asn Thr Leu Asn Asn Leu Gly His Ile 35 40 45 Tyr Val Arg His Val Ser Gly Ser Ser Pro His Pro Ile Thr Ser Thr 50 55 60 Ile Arg Ile Tyr Phe Lys Pro Lys His Thr Arg Ala Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Gln Tyr Lys Lys Ala Phe Ser Val Asp Phe Thr 85 90 95 Pro Thr Pro Ile Thr Asp Thr Arg Lys Asp Ile Asn Thr Val Thr Ser 100 105 110 Ile Gln Ser Gly Arg Arg Gly Asp Met Ser Ala Leu Asn Thr His Gly 115 120 125 Ala Phe Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Val 130 135 140 Val Asn Arg His Leu Ala Thr His Asn Asp Trp Gln Arg Cys Val Trp 145 150 155 160 Glu Asn Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly 165 170 175 Cys Asp Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Ile Tyr Tyr Cys 180 185 190 Ala Ser Lys Asn Lys His Tyr Pro Val Ala Phe Glu Gly Pro Gly Leu 195 200 205 Val Glu Val Gln Glu Asn Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His 210 215 220 Val Leu Leu Ala Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile 225 230 235 240 Leu Arg Cys Glu His Gly Val Ile Gly Ile 245 250 <210> SEQ ID NO 33 <211> LENGTH: 732 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(732) <223> OTHER INFORMATION: <400> SEQUENCE: 33 tgg aca gag ggc aac gcc ccc cca cgc atg tcc att cca ttc ata agt 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 ata ggg aat gcg tac agt aat ttt tac gat ggt tgg tca cac ttt tca 96 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 caa aac ggg gtg tat ggg tac aac gca tta aac aat atg ggc aag ttg 144 Gln Asn Gly Val Tyr Gly Tyr Asn Ala Leu Asn Asn Met Gly Lys Leu 35 40 45 tac gca cga cac gtg aac aaa gac aca cca tac caa atg tct agc acg 192 Tyr Ala Arg His Val Asn Lys Asp Thr Pro Tyr Gln Met Ser Ser Thr 50 55 60 atc cgt gtg tac ttt aag cct aag cac atc aga gtc tgg gtg ccg cga 240 Ile Arg Val Tyr Phe Lys Pro Lys His Ile Arg Val Trp Val Pro Arg 65 70 75 80 cca ccg cgc ttg tgt ccc tac atc aag tcc aac aac gtt aat ttt gac 288 Pro Pro Arg Leu Cys Pro Tyr Ile Lys Ser Asn Asn Val Asn Phe Asp 85 90 95 ccg acc aac cta act gac tca aga tca agc ata aca tac gtg ccc gat 336 Pro Thr Asn Leu Thr Asp Ser Arg Ser Ser Ile Thr Tyr Val Pro Asp 100 105 110 act atc cgt cca gag gtt cgc aca acc ggt agg ttt ggc caa cag tct 384 Thr Ile Arg Pro Glu Val Arg Thr Thr Gly Arg Phe Gly Gln Gln Ser 115 120 125 ggc gca atc tac gtg gga aat tat aga gtg gtg aac agg cat ctt gcc 432 Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 act cac aat gac tgg caa aat tgt gtg tgg gaa gag tat aac aga gac 480 Thr His Asn Asp Trp Gln Asn Cys Val Trp Glu Glu Tyr Asn Arg Asp 145 150 155 160 ctc ctt gtg agt acc acc aca gcc cat gga tgt gac acc atc gcc aga 528 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 tgc cag tgt acg aca ggt gtg tac ttt tgt gcc tca aga aac aaa cat 576 Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His 180 185 190 tat cca gtc acc ttt gaa ggg cca ggc tta gta gaa gtc cag gag agt 624 Tyr Pro Val Thr Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 gaa tac tac ccc aag agg tat cag tcc cat gtc ctc ttg gca gct ggg 672 Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly 210 215 220 ttc tcc gag cca ggt gac tgt ggc ggt ata ctg agg tgt gag cat ggt 720 Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly 225 230 235 240 gct atc ggt ctt 732 Ala Ile Gly Leu <210> SEQ ID NO 34 <211> LENGTH: 244 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 34 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Asn Gly Val Tyr Gly Tyr Asn Ala Leu Asn Asn Met Gly Lys Leu 35 40 45 Tyr Ala Arg His Val Asn Lys Asp Thr Pro Tyr Gln Met Ser Ser Thr 50 55 60 Ile Arg Val Tyr Phe Lys Pro Lys His Ile Arg Val Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Pro Tyr Ile Lys Ser Asn Asn Val Asn Phe Asp 85 90 95 Pro Thr Asn Leu Thr Asp Ser Arg Ser Ser Ile Thr Tyr Val Pro Asp 100 105 110 Thr Ile Arg Pro Glu Val Arg Thr Thr Gly Arg Phe Gly Gln Gln Ser 115 120 125 Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 Thr His Asn Asp Trp Gln Asn Cys Val Trp Glu Glu Tyr Asn Arg Asp 145 150 155 160 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His 180 185 190 Tyr Pro Val Thr Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly 210 215 220 Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly 225 230 235 240 Ala Ile Gly Leu <210> SEQ ID NO 35 <211> LENGTH: 750 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(750) <223> OTHER INFORMATION: <400> SEQUENCE: 35 tgg act gaa ggc aat gca cct ccc agg atg tcc att ccc ttt atg agc 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 atc gga aac gcg tat agc aat ttc tat gat ggg tgg tcc cac ttt tct 96 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 cag aat ggg gtt tat ggt tac aac aca tta aac cac atg ggg cag ctg 144 Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly Gln Leu 35 40 45 tac atg cgc cac gtg aac ggg cca tca cca ctg cca atg acc agc acg 192 Tyr Met Arg His Val Asn Gly Pro Ser Pro Leu Pro Met Thr Ser Thr 50 55 60 gtg aga gtg tac ttc aaa cca aag cat gtg aag gca tgg gtg cca cgg 240 Val Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 cct cca agg cta tgt cag tat aag aat gca tcg act gtg aac ttc tca 288 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ser Thr Val Asn Phe Ser 85 90 95 tct aca aac att act gac aag aga gac agc atc acc tac gta cca gac 336 Ser Thr Asn Ile Thr Asp Lys Arg Asp Ser Ile Thr Tyr Val Pro Asp 100 105 110 acc gtc aag cct gac gtg tcg aac cat ggc gcc ttt gga caa cag tct 384 Thr Val Lys Pro Asp Val Ser Asn His Gly Ala Phe Gly Gln Gln Ser 115 120 125 gga gct gtt tac gtg ggc aac tac aga gta gtc aac aga cac ctt gct 432 Gly Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 aca cac aac gat tgg caa aac tgt gtg tgg gaa gat tat aac agg gac 480 Thr His Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp 145 150 155 160 ctg ctg gtg agc acc act aca gca cac ggg tgc gac act att gcc aga 528 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 tgt caa tgc aat gct ggg gtg tac ttc tgt gct tca aaa aat aaa cac 576 Cys Gln Cys Asn Ala Gly Val Tyr Phe Cys Ala Ser Lys Asn Lys His 180 185 190 tac ccg att gtc ttc gaa ggt cca ggt ctt gtg gag gtg cag gag agt 624 Tyr Pro Ile Val Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 gaa tat tac cct aag aga tat caa tca cat gtg ttg ctc gct gca ggc 672 Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly 210 215 220 ttt tct gaa cca gga gat tgc ggt ggt atc ctg aga tgt gaa cac ggg 720 Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly 225 230 235 240 gtc att ggc atc gtg acc atg ggt ggt gaa 750 Val Ile Gly Ile Val Thr Met Gly Gly Glu 245 250 <210> SEQ ID NO 36 <211> LENGTH: 250 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 36 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly Gln Leu 35 40 45 Tyr Met Arg His Val Asn Gly Pro Ser Pro Leu Pro Met Thr Ser Thr 50 55 60 Val Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ser Thr Val Asn Phe Ser 85 90 95 Ser Thr Asn Ile Thr Asp Lys Arg Asp Ser Ile Thr Tyr Val Pro Asp 100 105 110 Thr Val Lys Pro Asp Val Ser Asn His Gly Ala Phe Gly Gln Gln Ser 115 120 125 Gly Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 Thr His Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp 145 150 155 160 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 Cys Gln Cys Asn Ala Gly Val Tyr Phe Cys Ala Ser Lys Asn Lys His 180 185 190 Tyr Pro Ile Val Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly 210 215 220 Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly 225 230 235 240 Val Ile Gly Ile Val Thr Met Gly Gly Glu 245 250 <210> SEQ ID NO 37 <211> LENGTH: 690 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(690) <223> OTHER INFORMATION: <400> SEQUENCE: 37 tgg act gag ggg aat gct cca ccc agg atg tcc atc cca ttt att agc 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 atc ggc aac gca tac agc agc ttc tat gat ggg tgg tca cac ttt gac 96 Ile Gly Asn Ala Tyr Ser Ser Phe Tyr Asp Gly Trp Ser His Phe Asp 20 25 30 agc aag ggt gca tac ggt ttc aac act ttg aac aaa atg ggg cac atc 144 Ser Lys Gly Ala Tyr Gly Phe Asn Thr Leu Asn Lys Met Gly His Ile 35 40 45 tat tgt aga cac gtg aac aag gaa acg cct gct gaa gtt acc agt tac 192 Tyr Cys Arg His Val Asn Lys Glu Thr Pro Ala Glu Val Thr Ser Tyr 50 55 60 atc agg att tac ttt aaa cct aag cat gtc aga gca tgg gtg cca agg 240 Ile Arg Ile Tyr Phe Lys Pro Lys His Val Arg Ala Trp Val Pro Arg 65 70 75 80 cct ccg cgg cta tgc cag tac aaa aac aag gca aat gtg aac ttt gac 288 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Lys Ala Asn Val Asn Phe Asp 85 90 95 gcc aca gcg gtt act gat acg cgt gct acc att aat act gtt cct gta 336 Ala Thr Ala Val Thr Asp Thr Arg Ala Thr Ile Asn Thr Val Pro Val 100 105 110 tca agt cat ggc ggt gaa cgc cgt ggt gac ctg gct gta ttg aat acg 384 Ser Ser His Gly Gly Glu Arg Arg Gly Asp Leu Ala Val Leu Asn Thr 115 120 125 cat ggt gct ttt ggc caa caa tcc gga gcc ata tat gtg ggc aat tac 432 His Gly Ala Phe Gly Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn Tyr 130 135 140 agg gtg gtt aac aga cat cta gct acg cac acg gac tgg cag aac tgc 480 Arg Val Val Asn Arg His Leu Ala Thr His Thr Asp Trp Gln Asn Cys 145 150 155 160 gtt tgg gaa gat tat aat agg gat ctc cta gtg agt aca aca acc gct 528 Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala 165 170 175 cat ggc tgt gac acg ata gct aga tgt caa tgt aaa gca ggt gta tac 576 His Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys Lys Ala Gly Val Tyr 180 185 190 tac tgc caa tcc aag agc aag cat tat ccg gtg tcc ttt gag ggg cca 624 Tyr Cys Gln Ser Lys Ser Lys His Tyr Pro Val Ser Phe Glu Gly Pro 195 200 205 ggt ctg gtg gaa gta cag gaa agt gag tac tac ccc aag aga tac caa 672 Gly Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln 210 215 220 tct cat gtg ctc ctt gcc 690 Ser His Val Leu Leu Ala 225 230 <210> SEQ ID NO 38 <211> LENGTH: 230 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 38 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Ser Phe Tyr Asp Gly Trp Ser His Phe Asp 20 25 30 Ser Lys Gly Ala Tyr Gly Phe Asn Thr Leu Asn Lys Met Gly His Ile 35 40 45 Tyr Cys Arg His Val Asn Lys Glu Thr Pro Ala Glu Val Thr Ser Tyr 50 55 60 Ile Arg Ile Tyr Phe Lys Pro Lys His Val Arg Ala Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Lys Ala Asn Val Asn Phe Asp 85 90 95 Ala Thr Ala Val Thr Asp Thr Arg Ala Thr Ile Asn Thr Val Pro Val 100 105 110 Ser Ser His Gly Gly Glu Arg Arg Gly Asp Leu Ala Val Leu Asn Thr 115 120 125 His Gly Ala Phe Gly Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn Tyr 130 135 140 Arg Val Val Asn Arg His Leu Ala Thr His Thr Asp Trp Gln Asn Cys 145 150 155 160 Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala 165 170 175 His Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys Lys Ala Gly Val Tyr 180 185 190 Tyr Cys Gln Ser Lys Ser Lys His Tyr Pro Val Ser Phe Glu Gly Pro 195 200 205 Gly Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln 210 215 220 Ser His Val Leu Leu Ala 225 230 <210> SEQ ID NO 39 <211> LENGTH: 762 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(762) <223> OTHER INFORMATION: <400> SEQUENCE: 39 tgg aca gag ggc aac gct ccc cca cgc atg tcc att cca ttc ata agt 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 ata ggg aat gcg tac agt aat ttt tac gat ggt tgg tca cac ttt tca 96 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 caa aac ggg gtg tat ggg tac aac gca ttg aac aat atg ggc aag ttg 144 Gln Asn Gly Val Tyr Gly Tyr Asn Ala Leu Asn Asn Met Gly Lys Leu 35 40 45 tac gca cga cac gtg aac aaa gac aca cca tac caa atg tct agc acg 192 Tyr Ala Arg His Val Asn Lys Asp Thr Pro Tyr Gln Met Ser Ser Thr 50 55 60 atc cgt gtg tac ttt aag ccc aag cac att aga gtc tgg gtg ccg cga 240 Ile Arg Val Tyr Phe Lys Pro Lys His Ile Arg Val Trp Val Pro Arg 65 70 75 80 cca ccg cgc ttg tgt ccc tat atc aag tcc agc aac gtt aat ttt gac 288 Pro Pro Arg Leu Cys Pro Tyr Ile Lys Ser Ser Asn Val Asn Phe Asp 85 90 95 ccg acc aac cta act gac tca aga tca agc ata aca tac gtg ccc gat 336 Pro Thr Asn Leu Thr Asp Ser Arg Ser Ser Ile Thr Tyr Val Pro Asp 100 105 110 act atc cgt cca gag gtt cgc aca acc ggt agg ttt ggc caa cag tct 384 Thr Ile Arg Pro Glu Val Arg Thr Thr Gly Arg Phe Gly Gln Gln Ser 115 120 125 ggc gca atc tac gtg gga aat tat aga gtg gtg aac aga cat ctc gcc 432 Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 act cac aat gac tgg caa aat tgt gtg tgg gaa gag tat aac aga gac 480 Thr His Asn Asp Trp Gln Asn Cys Val Trp Glu Glu Tyr Asn Arg Asp 145 150 155 160 ctc ctt gtg agt acc acc aca gcc cat gga tgt gac acc atc gcc aga 528 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 tgc cag tgt acg aca ggt gtg tac ttt tgt gcc tca aga aac aaa cat 576 Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His 180 185 190 tat cca gtc acc ttt gaa ggg cca ggt tta gta gaa gtc cag gag agt 624 Tyr Pro Val Thr Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 gaa tac tac ccc aag agg tat cag tcc cat gtc ctc tta gca gcc ggg 672 Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly 210 215 220 ttc tcc gag cca ggt gat tgt ggc ggt ata ttg agg tgt gag cat ggt 720 Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly 225 230 235 240 gtt atc ggt ctc gtt acc atg ggt ggc gaa ggt gtt gtg gga 762 Val Ile Gly Leu Val Thr Met Gly Gly Glu Gly Val Val Gly 245 250 <210> SEQ ID NO 40 <211> LENGTH: 254 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 40 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Asn Gly Val Tyr Gly Tyr Asn Ala Leu Asn Asn Met Gly Lys Leu 35 40 45 Tyr Ala Arg His Val Asn Lys Asp Thr Pro Tyr Gln Met Ser Ser Thr 50 55 60 Ile Arg Val Tyr Phe Lys Pro Lys His Ile Arg Val Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Pro Tyr Ile Lys Ser Ser Asn Val Asn Phe Asp 85 90 95 Pro Thr Asn Leu Thr Asp Ser Arg Ser Ser Ile Thr Tyr Val Pro Asp 100 105 110 Thr Ile Arg Pro Glu Val Arg Thr Thr Gly Arg Phe Gly Gln Gln Ser 115 120 125 Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 Thr His Asn Asp Trp Gln Asn Cys Val Trp Glu Glu Tyr Asn Arg Asp 145 150 155 160 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His 180 185 190 Tyr Pro Val Thr Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly 210 215 220 Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly 225 230 235 240 Val Ile Gly Leu Val Thr Met Gly Gly Glu Gly Val Val Gly 245 250 <210> SEQ ID NO 41 <211> LENGTH: 630 <212> TYPE: DNA <213> ORGANISM: Coxsackievirus B <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(630) <223> OTHER INFORMATION: <400> SEQUENCE: 41 aca gag ggc aac gca cca cca aga atg tcg ata ccg ttc atg agc ata 48 Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser Ile 1 5 10 15 ggt aat gcg tac acc atg ttc tat gac ggc tgg tca aat ttc tct agg 96 Gly Asn Ala Tyr Thr Met Phe Tyr Asp Gly Trp Ser Asn Phe Ser Arg 20 25 30 gat ggc ata tat ggt tac aat tca cta aat aac atg gga act att tat 144 Asp Gly Ile Tyr Gly Tyr Asn Ser Leu Asn Asn Met Gly Thr Ile Tyr 35 40 45 gcg cgt cat gtc aac gat tcc agc ccg ggt ggt tta acc agt acc att 192 Ala Arg His Val Asn Asp Ser Ser Pro Gly Gly Leu Thr Ser Thr Ile 50 55 60 cgc atc tac ttc aaa ccc aag cac gtc aag gcg tat gtg ccg cgt cca 240 Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Tyr Val Pro Arg Pro 65 70 75 80 cca cgc ttg tgc caa tac aag aag gca aaa aac gtg aac ttt gaa gtt 288 Pro Arg Leu Cys Gln Tyr Lys Lys Ala Lys Asn Val Asn Phe Glu Val 85 90 95 gag gcc gtt acc acc gag cgt gcg agt tta gtg acc acc ggt cca cac 336 Glu Ala Val Thr Thr Glu Arg Ala Ser Leu Val Thr Thr Gly Pro His 100 105 110 gga caa cag tct ggt gca gtg tat gtg ggc aac tac aga atc ata aac 384 Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Ile Ile Asn 115 120 125 aga cat ttg gca aca aac ttt gat tgg caa aat tgc gtg tgg gag gat 432 Arg His Leu Ala Thr Asn Phe Asp Trp Gln Asn Cys Val Trp Glu Asp 130 135 140 tac aac agg gac ctc cta gtg agc aca acc aca gcg cat ggg tgc gat 480 Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp 145 150 155 160 acc atc gct aga tgc cag tgt acc acg ggc gtg tac ttt tgt gca tcg 528 Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser 165 170 175 aag aac aag cac tat cca gtt gcc ttt gag ggc cca agt ctt gtg gaa 576 Lys Asn Lys His Tyr Pro Val Ala Phe Glu Gly Pro Ser Leu Val Glu 180 185 190 gtt caa gaa agt gaa tat tac ccc aaa agg tac cat tcc cat gta ctc 624 Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr His Ser His Val Leu 195 200 205 ctt gca 630 Leu Ala 210 <210> SEQ ID NO 42 <211> LENGTH: 210 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus B <400> SEQUENCE: 42 Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser Ile 1 5 10 15 Gly Asn Ala Tyr Thr Met Phe Tyr Asp Gly Trp Ser Asn Phe Ser Arg 20 25 30 Asp Gly Ile Tyr Gly Tyr Asn Ser Leu Asn Asn Met Gly Thr Ile Tyr 35 40 45 Ala Arg His Val Asn Asp Ser Ser Pro Gly Gly Leu Thr Ser Thr Ile 50 55 60 Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Tyr Val Pro Arg Pro 65 70 75 80 Pro Arg Leu Cys Gln Tyr Lys Lys Ala Lys Asn Val Asn Phe Glu Val 85 90 95 Glu Ala Val Thr Thr Glu Arg Ala Ser Leu Val Thr Thr Gly Pro His 100 105 110 Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Ile Ile Asn 115 120 125 Arg His Leu Ala Thr Asn Phe Asp Trp Gln Asn Cys Val Trp Glu Asp 130 135 140 Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp 145 150 155 160 Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser 165 170 175 Lys Asn Lys His Tyr Pro Val Ala Phe Glu Gly Pro Ser Leu Val Glu 180 185 190 Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr His Ser His Val Leu 195 200 205 Leu Ala 210 <210> SEQ ID NO 43 <211> LENGTH: 519 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(519) <223> OTHER INFORMATION: <400> SEQUENCE: 43 tgg acc gag ggc aat gca cct ccc agg atg tct atc ccc ttt atg agc 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 atc ggg aac gct tat agc aac ttc tat gat gga tgg tcc cac ttt tct 96 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 cag aat ggg gtt tat ggt tac aat aca tta aac cac atg ggg cag ttg 144 Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly Gln Leu 35 40 45 tac gtg cgc cac gtg aac gga cca tcg cca ctg cca atg acc agc aca 192 Tyr Val Arg His Val Asn Gly Pro Ser Pro Leu Pro Met Thr Ser Thr 50 55 60 gtg aga gtg tac ttc aaa cca aaa cat gtg aag gcg tgg gtg cca cga 240 Val Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 ccc cca agg cta tgt cag tac aag aat gca tca acc gtg aac ttc tca 288 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ser Thr Val Asn Phe Ser 85 90 95 tct aca aat att act gac aag aga gac agc atc acc tac ata cca gac 336 Ser Thr Asn Ile Thr Asp Lys Arg Asp Ser Ile Thr Tyr Ile Pro Asp 100 105 110 act gta aag cct gac gta tcg aac tac ggg gcg ttt ggt caa cag tcc 384 Thr Val Lys Pro Asp Val Ser Asn Tyr Gly Ala Phe Gly Gln Gln Ser 115 120 125 ggt gcc gtg tac gtg ggc aat tat aga gtg gtt aac aga cat tta gca 432 Gly Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 acg tac aat gat tgg caa aac tgt gtg tgg gaa gac tac aac aga gat 480 Thr Tyr Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp 145 150 155 160 ctc ttg gtc agc act acc aca gca cat ggg tgc gac acc 519 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr 165 170 <210> SEQ ID NO 44 <211> LENGTH: 173 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 44 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly Gln Leu 35 40 45 Tyr Val Arg His Val Asn Gly Pro Ser Pro Leu Pro Met Thr Ser Thr 50 55 60 Val Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ser Thr Val Asn Phe Ser 85 90 95 Ser Thr Asn Ile Thr Asp Lys Arg Asp Ser Ile Thr Tyr Ile Pro Asp 100 105 110 Thr Val Lys Pro Asp Val Ser Asn Tyr Gly Ala Phe Gly Gln Gln Ser 115 120 125 Gly Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 Thr Tyr Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp 145 150 155 160 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr 165 170 <210> SEQ ID NO 45 <211> LENGTH: 744 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(744) <223> OTHER INFORMATION: <400> SEQUENCE: 45 tgg acc gag ggc aat gca cct ccc agg atg tct atc ccc ttt atg agc 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 atc ggg aac gct tat agc aac ttc tat gat ggg tgg tcc cac ttt tct 96 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 cag aat ggg gtt tat ggt tac aat aca tta aac cac atg ggg cag ttg 144 Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly Gln Leu 35 40 45 tac gtg cgc cac gtg aac ggg cca tcg cca ctg cca atg acc agc aca 192 Tyr Val Arg His Val Asn Gly Pro Ser Pro Leu Pro Met Thr Ser Thr 50 55 60 gtg aga gtg tac ttc aaa cca aaa cat gtg aag gcg tgg gtg cca cga 240 Val Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 ccc cca agg cta tgt cag tac aag aat gca tca acc gta aac ttc tca 288 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ser Thr Val Asn Phe Ser 85 90 95 tct aca aat att act gac aag aga aat agc atc acc tac ata cca gac 336 Ser Thr Asn Ile Thr Asp Lys Arg Asn Ser Ile Thr Tyr Ile Pro Asp 100 105 110 act gta aag cct gac gtg tcg aac tac ggg gcg ttt ggt caa cag tcc 384 Thr Val Lys Pro Asp Val Ser Asn Tyr Gly Ala Phe Gly Gln Gln Ser 115 120 125 ggt gcc gtg tac gtg ggc aat tat aga gtg gtt aac aga cat tta gca 432 Gly Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 aca tac aat gat tgg caa aac tgt gtg tgg gaa gac tac aac aga gat 480 Thr Tyr Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp 145 150 155 160 ctc ttg gtc agc act acc aca gca cat gga tgc gac acc att gct agg 528 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 tgt cag tgc atg gca ggg gtg tac ttc tgt cga tcc agg aac aag cat 576 Cys Gln Cys Met Ala Gly Val Tyr Phe Cys Arg Ser Arg Asn Lys His 180 185 190 tac cca gtt tgt ttt gag ggt cca ggc cta gtg gag gtt caa gag agt 624 Tyr Pro Val Cys Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 gag tac tac ccc aag aga tat cag tcc cat gtg ttg tta gca gca gga 672 Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly 210 215 220 ctt tca gaa ccc gga gac tgt ggt ggc ata cta agg tgc cag cat ggc 720 Leu Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His Gly 225 230 235 240 gtc gtt ggc ata gtg acc atg gga 744 Val Val Gly Ile Val Thr Met Gly 245 <210> SEQ ID NO 46 <211> LENGTH: 248 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 46 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly Gln Leu 35 40 45 Tyr Val Arg His Val Asn Gly Pro Ser Pro Leu Pro Met Thr Ser Thr 50 55 60 Val Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ser Thr Val Asn Phe Ser 85 90 95 Ser Thr Asn Ile Thr Asp Lys Arg Asn Ser Ile Thr Tyr Ile Pro Asp 100 105 110 Thr Val Lys Pro Asp Val Ser Asn Tyr Gly Ala Phe Gly Gln Gln Ser 115 120 125 Gly Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 Thr Tyr Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp 145 150 155 160 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 Cys Gln Cys Met Ala Gly Val Tyr Phe Cys Arg Ser Arg Asn Lys His 180 185 190 Tyr Pro Val Cys Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly 210 215 220 Leu Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His Gly 225 230 235 240 Val Val Gly Ile Val Thr Met Gly 245 <210> SEQ ID NO 47 <211> LENGTH: 726 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(726) <223> OTHER INFORMATION: <400> SEQUENCE: 47 ttc tgg acc gag ggt aat gca cct ccc agg atg tct atc ccc ttt atg 48 Phe Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met 1 5 10 15 agc att ggg aac gct tat agc aac ttt tat gac ggg tgg tcc cac ttt 96 Ser Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe 20 25 30 tct cag aat ggg gta tat ggt tac aat aca tta aac cac atg ggg cag 144 Ser Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly Gln 35 40 45 ttg tac atg cgc cac gtg aat ggg cca tcg cca ctg cca atg acc agc 192 Leu Tyr Met Arg His Val Asn Gly Pro Ser Pro Leu Pro Met Thr Ser 50 55 60 aca gtg aga gtg tac ttc aaa cca aag cat gtg aag gca tgg gtg cca 240 Thr Val Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro 65 70 75 80 cga ccc cca agg cta tgt cag tac aag aat gca tca acc gtg aac ttt 288 Arg Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ser Thr Val Asn Phe 85 90 95 tca tct aca aat att act gac aag aga aac agc atc acc tac ata cca 336 Ser Ser Thr Asn Ile Thr Asp Lys Arg Asn Ser Ile Thr Tyr Ile Pro 100 105 110 gac act gtc aaa cct gac gtg tcg aac tac ggg gcg ttt ggt cag cag 384 Asp Thr Val Lys Pro Asp Val Ser Asn Tyr Gly Ala Phe Gly Gln Gln 115 120 125 tca ggc gct gtg tac gtg ggc aat tat aga gtg gtt aat aga cat ttg 432 Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu 130 135 140 gca aca tat aat gat tgg caa aac tgt gtg tgg gaa gac tat aac aga 480 Ala Thr Tyr Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg 145 150 155 160 gat ctc ttg gtc agc act acc aca gca cat ggg tgc gac acc att gct 528 Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala 165 170 175 agg tgt cag tgc atg gca ggg gtg tat ttt tgt cga tcc agg aac aag 576 Arg Cys Gln Cys Met Ala Gly Val Tyr Phe Cys Arg Ser Arg Asn Lys 180 185 190 cat tac cca gtt agc ttt gag ggt cca ggc ctg gtg gag gtt caa gag 624 His Tyr Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu 195 200 205 agt gag tac tac ccc aag aga tat cag tcc cat gtg ttg tta gcg gca 672 Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala 210 215 220 gga ctt tca gaa ccc gga gac tgt ggc ggc ata cta aga tgt gaa cat 720 Gly Leu Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His 225 230 235 240 ggc gtc 726 Gly Val <210> SEQ ID NO 48 <211> LENGTH: 242 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 48 Phe Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met 1 5 10 15 Ser Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe 20 25 30 Ser Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly Gln 35 40 45 Leu Tyr Met Arg His Val Asn Gly Pro Ser Pro Leu Pro Met Thr Ser 50 55 60 Thr Val Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro 65 70 75 80 Arg Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ser Thr Val Asn Phe 85 90 95 Ser Ser Thr Asn Ile Thr Asp Lys Arg Asn Ser Ile Thr Tyr Ile Pro 100 105 110 Asp Thr Val Lys Pro Asp Val Ser Asn Tyr Gly Ala Phe Gly Gln Gln 115 120 125 Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu 130 135 140 Ala Thr Tyr Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg 145 150 155 160 Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala 165 170 175 Arg Cys Gln Cys Met Ala Gly Val Tyr Phe Cys Arg Ser Arg Asn Lys 180 185 190 His Tyr Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu 195 200 205 Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala 210 215 220 Gly Leu Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His 225 230 235 240 Gly Val <210> SEQ ID NO 49 <211> LENGTH: 717 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(717) <223> OTHER INFORMATION: <400> SEQUENCE: 49 tgg acc gag ggt aat gca cct ccc agg atg tct atc ccc ttt atg agc 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 att ggg aac gct tat agc aac ttt tat gac ggg tgg tcc cac ttt tct 96 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 cag aat ggg gtt tat ggt tac aat aca tta aac cac atg ggg cag ttg 144 Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly Gln Leu 35 40 45 tac atg cgc cac gtg aac ggg cca tcg cca ctg cca atg acc agc aca 192 Tyr Met Arg His Val Asn Gly Pro Ser Pro Leu Pro Met Thr Ser Thr 50 55 60 gtg agg gtg tat ttc aaa cca aag cat gtg aag gca tgg gtg cca cga 240 Val Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 ccc cca agg cta tgt caa tac aag aat gca tca acc gtg aac ttt tca 288 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ser Thr Val Asn Phe Ser 85 90 95 tct aca aat att act gac aag aga ggc agc atc acc tac ata cca gac 336 Ser Thr Asn Ile Thr Asp Lys Arg Gly Ser Ile Thr Tyr Ile Pro Asp 100 105 110 acc gtc aaa cct gac gtg tcg aac tac ggg gcg ttt ggt cag cag tca 384 Thr Val Lys Pro Asp Val Ser Asn Tyr Gly Ala Phe Gly Gln Gln Ser 115 120 125 ggc gct gtg tac gtg ggc aat tat aga gtg gtt aac aga cat ttg gca 432 Gly Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 aca tat aat gat tgg caa aat tgt gtg tgg gaa gac tat aac aga gat 480 Thr Tyr Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp 145 150 155 160 ctc ttg gtc agc act acc aca gca cat ggg tgt gac acc att gct agg 528 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 tgt cgg tgc atg gca ggg gtg tat ttt tgt cga tcc agg aac aag cat 576 Cys Arg Cys Met Ala Gly Val Tyr Phe Cys Arg Ser Arg Asn Lys His 180 185 190 tac cca gtt agc ttt gag ggt cca ggc ctg gtg gag gtt caa gag agt 624 Tyr Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 gag tac tac ccc aag aga tat cag tcc cat gtg ttg tta gcg gca gga 672 Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly 210 215 220 ctt tca gaa ccc gga gac tgt ggt ggc ata cta aga tgt gaa cat 717 Leu Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His 225 230 235 <210> SEQ ID NO 50 <211> LENGTH: 239 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 50 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly Gln Leu 35 40 45 Tyr Met Arg His Val Asn Gly Pro Ser Pro Leu Pro Met Thr Ser Thr 50 55 60 Val Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ser Thr Val Asn Phe Ser 85 90 95 Ser Thr Asn Ile Thr Asp Lys Arg Gly Ser Ile Thr Tyr Ile Pro Asp 100 105 110 Thr Val Lys Pro Asp Val Ser Asn Tyr Gly Ala Phe Gly Gln Gln Ser 115 120 125 Gly Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 Thr Tyr Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp 145 150 155 160 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 Cys Arg Cys Met Ala Gly Val Tyr Phe Cys Arg Ser Arg Asn Lys His 180 185 190 Tyr Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly 210 215 220 Leu Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His 225 230 235 <210> SEQ ID NO 51 <211> LENGTH: 609 <212> TYPE: DNA <213> ORGANISM: Coxsackievirus B <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(609) <223> OTHER INFORMATION: <400> SEQUENCE: 51 acc cac caa ata atg tat gtg cca cca ggt ggc cca gtg cca gat aag 48 Thr His Gln Ile Met Tyr Val Pro Pro Gly Gly Pro Val Pro Asp Lys 1 5 10 15 gtt gac tca tat gtg tgg cag acg tcc aca aat ccc agt gtt ttc tgg 96 Val Asp Ser Tyr Val Trp Gln Thr Ser Thr Asn Pro Ser Val Phe Trp 20 25 30 acc gaa ggc aat gca ccg cca cgc atg tcc att cca ttt ttg agc att 144 Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Leu Ser Ile 35 40 45 ggc aac gca tac tct aac ttt tat gat ggc tgg tca gag ttt gcc aga 192 Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser Glu Phe Ala Arg 50 55 60 agc gga gtg tat ggc ata aac act ttg aac aat atg ggg acc ctt tac 240 Ser Gly Val Tyr Gly Ile Asn Thr Leu Asn Asn Met Gly Thr Leu Tyr 65 70 75 80 gca aga cat gtg aac gtt ggg agc aca gga cca ata aaa agt act att 288 Ala Arg His Val Asn Val Gly Ser Thr Gly Pro Ile Lys Ser Thr Ile 85 90 95 aga atc tac ttc aag cca aag cac gtt aag gca tgg ata cct aga cct 336 Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Ile Pro Arg Pro 100 105 110 cca agg ctg tgt cag tat gaa aaa gca aag aat gta aat ttt caa ccc 384 Pro Arg Leu Cys Gln Tyr Glu Lys Ala Lys Asn Val Asn Phe Gln Pro 115 120 125 agt ggt gta aca aca acc aga cag agc atc aca gca atg aca aat act 432 Ser Gly Val Thr Thr Thr Arg Gln Ser Ile Thr Ala Met Thr Asn Thr 130 135 140 ggg gcg ttc ggg cag caa tcg ggg gca ata tac gtg ggc aac tat agg 480 Gly Ala Phe Gly Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn Tyr Arg 145 150 155 160 gtg gtg aat aga cat ctg gct acg ttc aca gat tgg cag aat tgt gtg 528 Val Val Asn Arg His Leu Ala Thr Phe Thr Asp Trp Gln Asn Cys Val 165 170 175 tgg gaa gat tat aac agg gat cta ctt ggt agc act act acg ggc cat 576 Trp Glu Asp Tyr Asn Arg Asp Leu Leu Gly Ser Thr Thr Thr Gly His 180 185 190 ggg tgt gat gtc att gcc aga tgc cgg tgt aca 609 Gly Cys Asp Val Ile Ala Arg Cys Arg Cys Thr 195 200 <210> SEQ ID NO 52 <211> LENGTH: 203 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus B <400> SEQUENCE: 52 Thr His Gln Ile Met Tyr Val Pro Pro Gly Gly Pro Val Pro Asp Lys 1 5 10 15 Val Asp Ser Tyr Val Trp Gln Thr Ser Thr Asn Pro Ser Val Phe Trp 20 25 30 Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Leu Ser Ile 35 40 45 Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser Glu Phe Ala Arg 50 55 60 Ser Gly Val Tyr Gly Ile Asn Thr Leu Asn Asn Met Gly Thr Leu Tyr 65 70 75 80 Ala Arg His Val Asn Val Gly Ser Thr Gly Pro Ile Lys Ser Thr Ile 85 90 95 Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Ile Pro Arg Pro 100 105 110 Pro Arg Leu Cys Gln Tyr Glu Lys Ala Lys Asn Val Asn Phe Gln Pro 115 120 125 Ser Gly Val Thr Thr Thr Arg Gln Ser Ile Thr Ala Met Thr Asn Thr 130 135 140 Gly Ala Phe Gly Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn Tyr Arg 145 150 155 160 Val Val Asn Arg His Leu Ala Thr Phe Thr Asp Trp Gln Asn Cys Val 165 170 175 Trp Glu Asp Tyr Asn Arg Asp Leu Leu Gly Ser Thr Thr Thr Gly His 180 185 190 Gly Cys Asp Val Ile Ala Arg Cys Arg Cys Thr 195 200 <210> SEQ ID NO 53 <211> LENGTH: 720 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(720) <223> OTHER INFORMATION: <400> SEQUENCE: 53 aat gcc cct ccc agg atg tcg ata cca ttc atg agt gtt ggt aac gca 48 Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser Val Gly Asn Ala 1 5 10 15 tat tgc aac ttt tat gat gga tgg tct cat ttc agc cag agc ggc gtg 96 Tyr Cys Asn Phe Tyr Asp Gly Trp Ser His Phe Ser Gln Ser Gly Val 20 25 30 tac gga tac act act ttg aac aac atg gga cac ttg tat ttt aga cat 144 Tyr Gly Tyr Thr Thr Leu Asn Asn Met Gly His Leu Tyr Phe Arg His 35 40 45 gtg aac aaa tcg act gca tat cca gtt aac agt gtt gcc cgc gtc tat 192 Val Asn Lys Ser Thr Ala Tyr Pro Val Asn Ser Val Ala Arg Val Tyr 50 55 60 ttt aag ccc aag cac gtg aag gca tgg gtg cct cgt gca cca cgc ttg 240 Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg Ala Pro Arg Leu 65 70 75 80 tgc ccg tat ctg tat gca aga aat gtc aat ttt tat gtg caa ggg gta 288 Cys Pro Tyr Leu Tyr Ala Arg Asn Val Asn Phe Tyr Val Gln Gly Val 85 90 95 acc gaa tct cgg ggc aaa att act ctt gac cga tca act cac aat cct 336 Thr Glu Ser Arg Gly Lys Ile Thr Leu Asp Arg Ser Thr His Asn Pro 100 105 110 ttg tta aac act ggt gtt ttt ggg caa caa tca ggg gct gca tat gtg 384 Leu Leu Asn Thr Gly Val Phe Gly Gln Gln Ser Gly Ala Ala Tyr Val 115 120 125 ggc aac tac aga gta gtc aac aga cac ctc gcc aca cac gtt gac tgg 432 Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala Thr His Val Asp Trp 130 135 140 cag aat tgc gtg tgg gaa gac tac aat aga gac ttg tta gtt agc acc 480 Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr 145 150 155 160 acc aca gcc cac ggg tgt gac acc ata gct agg tgc cag tgc tcc aca 528 Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys Ser Thr 165 170 175 ggt gtg tac ttt tgc gca tcc agg aat aaa cat tac cca gtg tca ttt 576 Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His Tyr Pro Val Ser Phe 180 185 190 gaa ggg ccc ggg ctc gtt gag gtg cag gag agt gag tat tac cca aaa 624 Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys 195 200 205 agg tac cag tca cat gtg ttg ctc gcc gca agg ttt tct gag cca ggt 672 Arg Tyr Gln Ser His Val Leu Leu Ala Ala Arg Phe Ser Glu Pro Gly 210 215 220 gac tgt ggg ggc atc ttg aga tgc gag cat ggc gtc ata ggg ctt gtg 720 Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly Val Ile Gly Leu Val 225 230 235 240 <210> SEQ ID NO 54 <211> LENGTH: 240 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 54 Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser Val Gly Asn Ala 1 5 10 15 Tyr Cys Asn Phe Tyr Asp Gly Trp Ser His Phe Ser Gln Ser Gly Val 20 25 30 Tyr Gly Tyr Thr Thr Leu Asn Asn Met Gly His Leu Tyr Phe Arg His 35 40 45 Val Asn Lys Ser Thr Ala Tyr Pro Val Asn Ser Val Ala Arg Val Tyr 50 55 60 Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg Ala Pro Arg Leu 65 70 75 80 Cys Pro Tyr Leu Tyr Ala Arg Asn Val Asn Phe Tyr Val Gln Gly Val 85 90 95 Thr Glu Ser Arg Gly Lys Ile Thr Leu Asp Arg Ser Thr His Asn Pro 100 105 110 Leu Leu Asn Thr Gly Val Phe Gly Gln Gln Ser Gly Ala Ala Tyr Val 115 120 125 Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala Thr His Val Asp Trp 130 135 140 Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr 145 150 155 160 Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys Gln Cys Ser Thr 165 170 175 Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His Tyr Pro Val Ser Phe 180 185 190 Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys 195 200 205 Arg Tyr Gln Ser His Val Leu Leu Ala Ala Arg Phe Ser Glu Pro Gly 210 215 220 Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly Val Ile Gly Leu Val 225 230 235 240 <210> SEQ ID NO 55 <211> LENGTH: 831 <212> TYPE: DNA <213> ORGANISM: Coxsackievirus A <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(831) <223> OTHER INFORMATION: <400> SEQUENCE: 55 tgg aca gaa gga aac gca cca gcg cgc atg tca atc ccc ttt atc agc 48 Trp Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 ata ggg aat gct tat agc aac ttt tat gac ggg tgg tcg aac ttc gat 96 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser Asn Phe Asp 20 25 30 cag aag ggc tca tac ggg tac aat aca tta aac aac cta ggc cac ata 144 Gln Lys Gly Ser Tyr Gly Tyr Asn Thr Leu Asn Asn Leu Gly His Ile 35 40 45 tac gtg agg cat gtg agc ggg agt agc cct cat cca atc acg agt acc 192 Tyr Val Arg His Val Ser Gly Ser Ser Pro His Pro Ile Thr Ser Thr 50 55 60 atc agg ata tat ttt aaa cca aag cat act aga gcc tgg gtg cca cga 240 Ile Arg Ile Tyr Phe Lys Pro Lys His Thr Arg Ala Trp Val Pro Arg 65 70 75 80 cct ccg cga tta tgt cag tat aag aag gca ttc agt gta gat ttt aca 288 Pro Pro Arg Leu Cys Gln Tyr Lys Lys Ala Phe Ser Val Asp Phe Thr 85 90 95 cca act cct att act gac acc agg aaa gac atc aac acc gta acc tca 336 Pro Thr Pro Ile Thr Asp Thr Arg Lys Asp Ile Asn Thr Val Thr Ser 100 105 110 ata caa agt ggg cgc cga ggt gac atg tcc gca cta aac acc cat ggg 384 Ile Gln Ser Gly Arg Arg Gly Asp Met Ser Ala Leu Asn Thr His Gly 115 120 125 gcg ttt gga cag caa tct gga gcg gtg tat gtg gga aac tat aga gtg 432 Ala Phe Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Val 130 135 140 gtg aac aga cac ctt gcc aca cgc aat gac tgg caa aac tgt gtg tgg 480 Val Asn Arg His Leu Ala Thr Arg Asn Asp Trp Gln Asn Cys Val Trp 145 150 155 160 gag gat tat aat aga gac ctt cta gtg agc aca acc aat gca cat ggc 528 Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Asn Ala His Gly 165 170 175 tgt gac act ata gcc aga tgt caa tgt acc aca ggt gtg tat ttc tgt 576 Cys Asp Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys 180 185 190 gct tca agg aat aag cat tac cca gtg agt ttt gaa gga cca ggc tta 624 Ala Ser Arg Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro Gly Leu 195 200 205 gtg gaa gtt cag gaa agt gag tac tac ccc aag agg tac caa tcc cat 672 Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His 210 215 220 gtt ctc ctg gca gcg ggt ttc tct gag ccc ggt gat tgt ggt ggc atc 720 Val Leu Leu Ala Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile 225 230 235 240 ttg agg tgt gag cac gga gtc atc ggc att gtg act atg ggt ggt gaa 768 Leu Arg Cys Glu His Gly Val Ile Gly Ile Val Thr Met Gly Gly Glu 245 250 255 ggc gtc gtc ggt ttt gct gat gtg cga gac ctc ttg tgg tta gaa gat 816 Gly Val Val Gly Phe Ala Asp Val Arg Asp Leu Leu Trp Leu Glu Asp 260 265 270 gat gct atg gaa cag 831 Asp Ala Met Glu Gln 275 <210> SEQ ID NO 56 <211> LENGTH: 277 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus A <400> SEQUENCE: 56 Trp Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser Asn Phe Asp 20 25 30 Gln Lys Gly Ser Tyr Gly Tyr Asn Thr Leu Asn Asn Leu Gly His Ile 35 40 45 Tyr Val Arg His Val Ser Gly Ser Ser Pro His Pro Ile Thr Ser Thr 50 55 60 Ile Arg Ile Tyr Phe Lys Pro Lys His Thr Arg Ala Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Gln Tyr Lys Lys Ala Phe Ser Val Asp Phe Thr 85 90 95 Pro Thr Pro Ile Thr Asp Thr Arg Lys Asp Ile Asn Thr Val Thr Ser 100 105 110 Ile Gln Ser Gly Arg Arg Gly Asp Met Ser Ala Leu Asn Thr His Gly 115 120 125 Ala Phe Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Val 130 135 140 Val Asn Arg His Leu Ala Thr Arg Asn Asp Trp Gln Asn Cys Val Trp 145 150 155 160 Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Asn Ala His Gly 165 170 175 Cys Asp Thr Ile Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys 180 185 190 Ala Ser Arg Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro Gly Leu 195 200 205 Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His 210 215 220 Val Leu Leu Ala Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile 225 230 235 240 Leu Arg Cys Glu His Gly Val Ile Gly Ile Val Thr Met Gly Gly Glu 245 250 255 Gly Val Val Gly Phe Ala Asp Val Arg Asp Leu Leu Trp Leu Glu Asp 260 265 270 Asp Ala Met Glu Gln 275 <210> SEQ ID NO 57 <211> LENGTH: 666 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(666) <223> OTHER INFORMATION: <400> SEQUENCE: 57 aca gag ggc aac gcc cct gct cgc atg tca ata ccc ttc atg agt gtt 48 Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe Met Ser Val 1 5 10 15 ggc aac gct tac tgc aac ttc tat gat gga tgg tct cac ttt tca caa 96 Gly Asn Ala Tyr Cys Asn Phe Tyr Asp Gly Trp Ser His Phe Ser Gln 20 25 30 tct ggt gtt tat ggc ttc acc acc ctc aac aac atg ggc caa ttg ttc 144 Ser Gly Val Tyr Gly Phe Thr Thr Leu Asn Asn Met Gly Gln Leu Phe 35 40 45 ttc cgc cat gtg aat aaa gac acc ctt ggt ccc tat aac agt act gtg 192 Phe Arg His Val Asn Lys Asp Thr Leu Gly Pro Tyr Asn Ser Thr Val 50 55 60 aga gtt tac ttc aaa cca aag cac att aaa gca tgg gtg ccc agg cca 240 Arg Val Tyr Phe Lys Pro Lys His Ile Lys Ala Trp Val Pro Arg Pro 65 70 75 80 cca cgc ctg tgt gac tac gta tac gca cac aac gtt gac ttc aac cct 288 Pro Arg Leu Cys Asp Tyr Val Tyr Ala His Asn Val Asp Phe Asn Pro 85 90 95 aaa gga gtc acg gag agt aga gac aag atc aca ctg gag aga gat gac 336 Lys Gly Val Thr Glu Ser Arg Asp Lys Ile Thr Leu Glu Arg Asp Asp 100 105 110 cac ttg ccc tca atg gtt agc cat ggg gca ttt gga cag caa tct ggt 384 His Leu Pro Ser Met Val Ser His Gly Ala Phe Gly Gln Gln Ser Gly 115 120 125 gct gtg tac gta ggc aat tac agg gtt gtc aat aga cac cta gca act 432 Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala Thr 130 135 140 cac gct gac tgg caa aac tgc gtg tgg gag gac tac aat aga gat ttg 480 His Ala Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu 145 150 155 160 ctg gtt agc acc acc aca gcc cat ggt tgc gac act ata gct aga tgt 528 Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys 165 170 175 cag tgc acc aca ggt gtc tat ttt tgc gcc tcg aga aac aag cat tat 576 Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His Tyr 180 185 190 cca gta agt ttt gag ggg ccc ggt tta gtt gag gtg cag gag agt gag 624 Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu 195 200 205 tac tac cct aaa aga tac cag tcc cat gta ttg cta gca gca 666 Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala 210 215 220 <210> SEQ ID NO 58 <211> LENGTH: 222 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 58 Thr Glu Gly Asn Ala Pro Ala Arg Met Ser Ile Pro Phe Met Ser Val 1 5 10 15 Gly Asn Ala Tyr Cys Asn Phe Tyr Asp Gly Trp Ser His Phe Ser Gln 20 25 30 Ser Gly Val Tyr Gly Phe Thr Thr Leu Asn Asn Met Gly Gln Leu Phe 35 40 45 Phe Arg His Val Asn Lys Asp Thr Leu Gly Pro Tyr Asn Ser Thr Val 50 55 60 Arg Val Tyr Phe Lys Pro Lys His Ile Lys Ala Trp Val Pro Arg Pro 65 70 75 80 Pro Arg Leu Cys Asp Tyr Val Tyr Ala His Asn Val Asp Phe Asn Pro 85 90 95 Lys Gly Val Thr Glu Ser Arg Asp Lys Ile Thr Leu Glu Arg Asp Asp 100 105 110 His Leu Pro Ser Met Val Ser His Gly Ala Phe Gly Gln Gln Ser Gly 115 120 125 Ala Val Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala Thr 130 135 140 His Ala Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu 145 150 155 160 Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys 165 170 175 Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His Tyr 180 185 190 Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu 195 200 205 Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala Ala 210 215 220 <210> SEQ ID NO 59 <211> LENGTH: 774 <212> TYPE: DNA <213> ORGANISM: Coxsackievirus A <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(774) <223> OTHER INFORMATION: <400> SEQUENCE: 59 gtg tca gtt ccc ttc atg tca cca gcc agt gca tat caa tgg ttt tac 48 Val Ser Val Pro Phe Met Ser Pro Ala Ser Ala Tyr Gln Trp Phe Tyr 1 5 10 15 gat ggc tac cct act ttt gga gag cac ctc caa gca aat gac cta gat 96 Asp Gly Tyr Pro Thr Phe Gly Glu His Leu Gln Ala Asn Asp Leu Asp 20 25 30 tat ggc cag tgt cct aat aac atg atg ggc acc ttt agc att agg aca 144 Tyr Gly Gln Cys Pro Asn Asn Met Met Gly Thr Phe Ser Ile Arg Thr 35 40 45 gta ggg act gaa aag tca cca cac tcc att acc ctg aga gtg tac atg 192 Val Gly Thr Glu Lys Ser Pro His Ser Ile Thr Leu Arg Val Tyr Met 50 55 60 aga att aaa cac gtc agg gcg tgg ata cca agg cct ctg aga aac caa 240 Arg Ile Lys His Val Arg Ala Trp Ile Pro Arg Pro Leu Arg Asn Gln 65 70 75 80 ccc tat cta ttt aag acc aac cca aat tat aaa gga aat gat atc aaa 288 Pro Tyr Leu Phe Lys Thr Asn Pro Asn Tyr Lys Gly Asn Asp Ile Lys 85 90 95 tgc act agc acc agt aga gac aag atc acg acc tta ggg aag ttt gga 336 Cys Thr Ser Thr Ser Arg Asp Lys Ile Thr Thr Leu Gly Lys Phe Gly 100 105 110 cag caa tca ggc gcc ata tat gtg ggc aat tat agg gta gtg aat cga 384 Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn Arg 115 120 125 cac ctc gca aca cac aac gat tgg gca aac ctc gtg tgg gaa gac agc 432 His Leu Ala Thr His Asn Asp Trp Ala Asn Leu Val Trp Glu Asp Ser 130 135 140 tct agg gat ctg tta gtc tcc tcc acc acc gcc cag ggg tgc gat act 480 Ser Arg Asp Leu Leu Val Ser Ser Thr Thr Ala Gln Gly Cys Asp Thr 145 150 155 160 att gct aga tgc aat tgc cag acc ggg gtg tac tac tgt aac tcc aag 528 Ile Ala Arg Cys Asn Cys Gln Thr Gly Val Tyr Tyr Cys Asn Ser Lys 165 170 175 aga aaa cat tat cca gtt agt ttc act aag cct agc ttg ata ttt gta 576 Arg Lys His Tyr Pro Val Ser Phe Thr Lys Pro Ser Leu Ile Phe Val 180 185 190 gaa gct agc gag tac tac cca gct agg tac cag tca cac ctc atg ctt 624 Glu Ala Ser Glu Tyr Tyr Pro Ala Arg Tyr Gln Ser His Leu Met Leu 195 200 205 gct cag ggt cac tca gaa cct ggc gac tgt ggt ggc att ctt aga tgc 672 Ala Gln Gly His Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys 210 215 220 cag cac ggt gta gta gga ctt gtt tcc act ggt ggg aat ggc ctt gta 720 Gln His Gly Val Val Gly Leu Val Ser Thr Gly Gly Asn Gly Leu Val 225 230 235 240 ggg ttt gct gat att aga gac ctt tta tgg cta gat gag gaa gca atg 768 Gly Phe Ala Asp Ile Arg Asp Leu Leu Trp Leu Asp Glu Glu Ala Met 245 250 255 gaa cag 774 Glu Gln <210> SEQ ID NO 60 <211> LENGTH: 258 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus A <400> SEQUENCE: 60 Val Ser Val Pro Phe Met Ser Pro Ala Ser Ala Tyr Gln Trp Phe Tyr 1 5 10 15 Asp Gly Tyr Pro Thr Phe Gly Glu His Leu Gln Ala Asn Asp Leu Asp 20 25 30 Tyr Gly Gln Cys Pro Asn Asn Met Met Gly Thr Phe Ser Ile Arg Thr 35 40 45 Val Gly Thr Glu Lys Ser Pro His Ser Ile Thr Leu Arg Val Tyr Met 50 55 60 Arg Ile Lys His Val Arg Ala Trp Ile Pro Arg Pro Leu Arg Asn Gln 65 70 75 80 Pro Tyr Leu Phe Lys Thr Asn Pro Asn Tyr Lys Gly Asn Asp Ile Lys 85 90 95 Cys Thr Ser Thr Ser Arg Asp Lys Ile Thr Thr Leu Gly Lys Phe Gly 100 105 110 Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn Arg 115 120 125 His Leu Ala Thr His Asn Asp Trp Ala Asn Leu Val Trp Glu Asp Ser 130 135 140 Ser Arg Asp Leu Leu Val Ser Ser Thr Thr Ala Gln Gly Cys Asp Thr 145 150 155 160 Ile Ala Arg Cys Asn Cys Gln Thr Gly Val Tyr Tyr Cys Asn Ser Lys 165 170 175 Arg Lys His Tyr Pro Val Ser Phe Thr Lys Pro Ser Leu Ile Phe Val 180 185 190 Glu Ala Ser Glu Tyr Tyr Pro Ala Arg Tyr Gln Ser His Leu Met Leu 195 200 205 Ala Gln Gly His Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys 210 215 220 Gln His Gly Val Val Gly Leu Val Ser Thr Gly Gly Asn Gly Leu Val 225 230 235 240 Gly Phe Ala Asp Ile Arg Asp Leu Leu Trp Leu Asp Glu Glu Ala Met 245 250 255 Glu Gln <210> SEQ ID NO 61 <211> LENGTH: 717 <212> TYPE: DNA <213> ORGANISM: Coxsackievirus A <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(717) <223> OTHER INFORMATION: <400> SEQUENCE: 61 gca tat caa agg ttt tac gat ggt tac cct act ttt gga gag cac ctt 48 Ala Tyr Gln Arg Phe Tyr Asp Gly Tyr Pro Thr Phe Gly Glu His Leu 1 5 10 15 caa gca aat gac cta tat tat ggc cag tgc cct aat aac atg atg ggc 96 Gln Ala Asn Asp Leu Tyr Tyr Gly Gln Cys Pro Asn Asn Met Met Gly 20 25 30 acc ttt agc att agg aca gta ggg act gaa aag tca cca cac tcc att 144 Thr Phe Ser Ile Arg Thr Val Gly Thr Glu Lys Ser Pro His Ser Ile 35 40 45 acc ctg aga gtg tac atg aga att aaa cac gtc agg gcg tgg ata cca 192 Thr Leu Arg Val Tyr Met Arg Ile Lys His Val Arg Ala Trp Ile Pro 50 55 60 agg cct ctg aga aac caa ccc tat cta ttt aag acc aac cca aat tat 240 Arg Pro Leu Arg Asn Gln Pro Tyr Leu Phe Lys Thr Asn Pro Asn Tyr 65 70 75 80 aaa gga aat gat atc aaa tgc act agc acc agt aga gac aag atc acg 288 Lys Gly Asn Asp Ile Lys Cys Thr Ser Thr Ser Arg Asp Lys Ile Thr 85 90 95 acc tta ggg aag ttt gga cag caa tca ggc gcc ata tat gtg ggc aat 336 Thr Leu Gly Lys Phe Gly Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn 100 105 110 tat agg gta gtg aat cga cac ctc gca aca cac aac gat tgg gca aac 384 Tyr Arg Val Val Asn Arg His Leu Ala Thr His Asn Asp Trp Ala Asn 115 120 125 ctc gtg tgg gaa gac agc tct agg gat ctg tta gtc tcc tcc acc acc 432 Leu Val Trp Glu Asp Ser Ser Arg Asp Leu Leu Val Ser Ser Thr Thr 130 135 140 gcc cag ggg tgc gat act att gct aga tgc aat tgc cag acc ggg gtg 480 Ala Gln Gly Cys Asp Thr Ile Ala Arg Cys Asn Cys Gln Thr Gly Val 145 150 155 160 tac tac tgt aac tcc aag aga aaa cat tat cca gtt agt ttc act aag 528 Tyr Tyr Cys Asn Ser Lys Arg Lys His Tyr Pro Val Ser Phe Thr Lys 165 170 175 cct agc ttg ata ttt gta gaa gct agc gag tac tac cca gct agg tac 576 Pro Ser Leu Ile Phe Val Glu Ala Ser Glu Tyr Tyr Pro Ala Arg Tyr 180 185 190 cag tca cac ctc atg ctt gct cag ggt cac tca gaa cct ggc gac tgt 624 Gln Ser His Leu Met Leu Ala Gln Gly His Ser Glu Pro Gly Asp Cys 195 200 205 ggt ggc att ctt aga tgc cag cac ggt gta gta gga ctt ggt tcc act 672 Gly Gly Ile Leu Arg Cys Gln His Gly Val Val Gly Leu Gly Ser Thr 210 215 220 ggt ggg aat ggc ctt gta ggg ttt gct gat att aga gac ctt ttt 717 Gly Gly Asn Gly Leu Val Gly Phe Ala Asp Ile Arg Asp Leu Phe 225 230 235 <210> SEQ ID NO 62 <211> LENGTH: 239 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus A <400> SEQUENCE: 62 Ala Tyr Gln Arg Phe Tyr Asp Gly Tyr Pro Thr Phe Gly Glu His Leu 1 5 10 15 Gln Ala Asn Asp Leu Tyr Tyr Gly Gln Cys Pro Asn Asn Met Met Gly 20 25 30 Thr Phe Ser Ile Arg Thr Val Gly Thr Glu Lys Ser Pro His Ser Ile 35 40 45 Thr Leu Arg Val Tyr Met Arg Ile Lys His Val Arg Ala Trp Ile Pro 50 55 60 Arg Pro Leu Arg Asn Gln Pro Tyr Leu Phe Lys Thr Asn Pro Asn Tyr 65 70 75 80 Lys Gly Asn Asp Ile Lys Cys Thr Ser Thr Ser Arg Asp Lys Ile Thr 85 90 95 Thr Leu Gly Lys Phe Gly Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn 100 105 110 Tyr Arg Val Val Asn Arg His Leu Ala Thr His Asn Asp Trp Ala Asn 115 120 125 Leu Val Trp Glu Asp Ser Ser Arg Asp Leu Leu Val Ser Ser Thr Thr 130 135 140 Ala Gln Gly Cys Asp Thr Ile Ala Arg Cys Asn Cys Gln Thr Gly Val 145 150 155 160 Tyr Tyr Cys Asn Ser Lys Arg Lys His Tyr Pro Val Ser Phe Thr Lys 165 170 175 Pro Ser Leu Ile Phe Val Glu Ala Ser Glu Tyr Tyr Pro Ala Arg Tyr 180 185 190 Gln Ser His Leu Met Leu Ala Gln Gly His Ser Glu Pro Gly Asp Cys 195 200 205 Gly Gly Ile Leu Arg Cys Gln His Gly Val Val Gly Leu Gly Ser Thr 210 215 220 Gly Gly Asn Gly Leu Val Gly Phe Ala Asp Ile Arg Asp Leu Phe 225 230 235 <210> SEQ ID NO 63 <211> LENGTH: 756 <212> TYPE: DNA <213> ORGANISM: Unknown <220> FEATURE: <223> OTHER INFORMATION: one of six Madagascan enterovirus isolates, not neutralizable by PDVM pools, therefore untypeable <221> NAME/KEY: CDS <222> LOCATION: (1)..(756) <223> OTHER INFORMATION: <400> SEQUENCE: 63 tac act tat ggc aca gca cca cct agg att tca ata cct tac atc ggt 48 Tyr Thr Tyr Gly Thr Ala Pro Pro Arg Ile Ser Ile Pro Tyr Ile Gly 1 5 10 15 atc gcc aat gct tac tcc cac ttc tac gat ggg tat gca gtt gtg ccg 96 Ile Ala Asn Ala Tyr Ser His Phe Tyr Asp Gly Tyr Ala Val Val Pro 20 25 30 tta aaa gat tcc gta caa gac tca gga gct gcg tat tac ggt gca gtg 144 Leu Lys Asp Ser Val Gln Asp Ser Gly Ala Ala Tyr Tyr Gly Ala Val 35 40 45 tcc att aat gat ttt ggc ctt ctt gca gtt cga gtg gtt aat gag cat 192 Ser Ile Asn Asp Phe Gly Leu Leu Ala Val Arg Val Val Asn Glu His 50 55 60 aac cct gtc aga gta tca tcg aag gtt aga gta tac atg aaa cca aaa 240 Asn Pro Val Arg Val Ser Ser Lys Val Arg Val Tyr Met Lys Pro Lys 65 70 75 80 cat gtc aga gtg tgg tgt cca cgc cca cca cgt gcc gtg gag tac tac 288 His Val Arg Val Trp Cys Pro Arg Pro Pro Arg Ala Val Glu Tyr Tyr 85 90 95 gga cct ggg gtg gat tac aag gca gac aca tta aca cca cta cca acc 336 Gly Pro Gly Val Asp Tyr Lys Ala Asp Thr Leu Thr Pro Leu Pro Thr 100 105 110 aag aat cta acc aca tat ggc ttt ggt cac caa aac aag gca ata tat 384 Lys Asn Leu Thr Thr Tyr Gly Phe Gly His Gln Asn Lys Ala Ile Tyr 115 120 125 gtt gca ggg tat aag ata gtg aat tac cac ttg gcc acg gca gag gat 432 Val Ala Gly Tyr Lys Ile Val Asn Tyr His Leu Ala Thr Ala Glu Asp 130 135 140 ttt agg cac tgt gtt aga agc atg tgg gag cgt gac atc atg ata gtg 480 Phe Arg His Cys Val Arg Ser Met Trp Glu Arg Asp Ile Met Ile Val 145 150 155 160 gag tcc aaa gca caa ggg aaa gac caa att gcc aga tgt gcg tgc cga 528 Glu Ser Lys Ala Gln Gly Lys Asp Gln Ile Ala Arg Cys Ala Cys Arg 165 170 175 tca gga gtg tac tac tgt gaa tca aga gga aag cac tac ccc att aca 576 Ser Gly Val Tyr Tyr Cys Glu Ser Arg Gly Lys His Tyr Pro Ile Thr 180 185 190 att aca caa ccc acc ttt cag tgg atg gag aag aat gat ttc tac cca 624 Ile Thr Gln Pro Thr Phe Gln Trp Met Glu Lys Asn Asp Phe Tyr Pro 195 200 205 gca cgc tat caa tca cac atg aca att ggc cac ggt ttt gca gcc cct 672 Ala Arg Tyr Gln Ser His Met Thr Ile Gly His Gly Phe Ala Ala Pro 210 215 220 ggg gac tgt ggt ggc atc tta cgg tgc caa cac ggt gtc atg ggc cta 720 Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His Gly Val Met Gly Leu 225 230 235 240 ata act gct ggt ggt aat gga ctg gta gca ttc gca 756 Ile Thr Ala Gly Gly Asn Gly Leu Val Ala Phe Ala 245 250 <210> SEQ ID NO 64 <211> LENGTH: 252 <212> TYPE: PRT <213> ORGANISM: Unknown <220> FEATURE: <223> OTHER INFORMATION: one of six Madagascan enterovirus isolates, not neutralizable by PDVM pools, therefore untypeable <400> SEQUENCE: 64 Tyr Thr Tyr Gly Thr Ala Pro Pro Arg Ile Ser Ile Pro Tyr Ile Gly 1 5 10 15 Ile Ala Asn Ala Tyr Ser His Phe Tyr Asp Gly Tyr Ala Val Val Pro 20 25 30 Leu Lys Asp Ser Val Gln Asp Ser Gly Ala Ala Tyr Tyr Gly Ala Val 35 40 45 Ser Ile Asn Asp Phe Gly Leu Leu Ala Val Arg Val Val Asn Glu His 50 55 60 Asn Pro Val Arg Val Ser Ser Lys Val Arg Val Tyr Met Lys Pro Lys 65 70 75 80 His Val Arg Val Trp Cys Pro Arg Pro Pro Arg Ala Val Glu Tyr Tyr 85 90 95 Gly Pro Gly Val Asp Tyr Lys Ala Asp Thr Leu Thr Pro Leu Pro Thr 100 105 110 Lys Asn Leu Thr Thr Tyr Gly Phe Gly His Gln Asn Lys Ala Ile Tyr 115 120 125 Val Ala Gly Tyr Lys Ile Val Asn Tyr His Leu Ala Thr Ala Glu Asp 130 135 140 Phe Arg His Cys Val Arg Ser Met Trp Glu Arg Asp Ile Met Ile Val 145 150 155 160 Glu Ser Lys Ala Gln Gly Lys Asp Gln Ile Ala Arg Cys Ala Cys Arg 165 170 175 Ser Gly Val Tyr Tyr Cys Glu Ser Arg Gly Lys His Tyr Pro Ile Thr 180 185 190 Ile Thr Gln Pro Thr Phe Gln Trp Met Glu Lys Asn Asp Phe Tyr Pro 195 200 205 Ala Arg Tyr Gln Ser His Met Thr Ile Gly His Gly Phe Ala Ala Pro 210 215 220 Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His Gly Val Met Gly Leu 225 230 235 240 Ile Thr Ala Gly Gly Asn Gly Leu Val Ala Phe Ala 245 250 <210> SEQ ID NO 65 <211> LENGTH: 726 <212> TYPE: DNA <213> ORGANISM: Unknown <220> FEATURE: <223> OTHER INFORMATION: one of six Madagascan enterovirus isolates, not neutralizable by PDVM pools, therefore untypeable <221> NAME/KEY: CDS <222> LOCATION: (1)..(726) <223> OTHER INFORMATION: <400> SEQUENCE: 65 tac act tat cgc aca gca cca cct agg att tca ata cct tac atc ggt 48 Tyr Thr Tyr Arg Thr Ala Pro Pro Arg Ile Ser Ile Pro Tyr Ile Gly 1 5 10 15 atc gcc aat gct tac tcc cac ttc tac gat ggg tat gca gtt gtg ccg 96 Ile Ala Asn Ala Tyr Ser His Phe Tyr Asp Gly Tyr Ala Val Val Pro 20 25 30 cta aaa gat tcc gca caa gac tca gga gct gcg tat tac ggt gca gtg 144 Leu Lys Asp Ser Ala Gln Asp Ser Gly Ala Ala Tyr Tyr Gly Ala Val 35 40 45 tcc att aat gac ttt ggc ctt ctt gca gtt cga gtg gtt aac gag cat 192 Ser Ile Asn Asp Phe Gly Leu Leu Ala Val Arg Val Val Asn Glu His 50 55 60 aac cct gtc aga gta tca tcg aag gtt aga gta tac atg aaa cca aaa 240 Asn Pro Val Arg Val Ser Ser Lys Val Arg Val Tyr Met Lys Pro Lys 65 70 75 80 cac gtc aga gtg tgg tgt cca cgc cca cca cgt gcc gtg gag tac tac 288 His Val Arg Val Trp Cys Pro Arg Pro Pro Arg Ala Val Glu Tyr Tyr 85 90 95 gga cct ggg gtg gat tac aag gca gac aca tta aca cca cta cca act 336 Gly Pro Gly Val Asp Tyr Lys Ala Asp Thr Leu Thr Pro Leu Pro Thr 100 105 110 aag aat cta acc aca tat ggc ttt ggt cac caa aac aag gca ata tat 384 Lys Asn Leu Thr Thr Tyr Gly Phe Gly His Gln Asn Lys Ala Ile Tyr 115 120 125 gtt gca ggg tat aag ata gtg aat tac cac ctg gcc acg gca gag gat 432 Val Ala Gly Tyr Lys Ile Val Asn Tyr His Leu Ala Thr Ala Glu Asp 130 135 140 ttt agg cac tgt gtt aga agc atg tgg gag cgt gac atc atg ata gtg 480 Phe Arg His Cys Val Arg Ser Met Trp Glu Arg Asp Ile Met Ile Val 145 150 155 160 gag tcc aaa gca caa ggg aga gac caa att gcc aga tgt gcg tgc cga 528 Glu Ser Lys Ala Gln Gly Arg Asp Gln Ile Ala Arg Cys Ala Cys Arg 165 170 175 tca gga gtg tac tac tgt gaa tca aga gga aag cac tac ccc att aca 576 Ser Gly Val Tyr Tyr Cys Glu Ser Arg Gly Lys His Tyr Pro Ile Thr 180 185 190 att aca caa ccc acc ttt cag tgg atg gag aag aat gat ttc tac cca 624 Ile Thr Gln Pro Thr Phe Gln Trp Met Glu Lys Asn Asp Phe Tyr Pro 195 200 205 gca cgc tat caa tca cac atg aca att ggc cac ggt ttt gca gcc cct 672 Ala Arg Tyr Gln Ser His Met Thr Ile Gly His Gly Phe Ala Ala Pro 210 215 220 ggg gac tgt ggt ggc atc tta cgg tgc caa cac ggt gtc atg ggc cta 720 Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His Gly Val Met Gly Leu 225 230 235 240 ata act 726 Ile Thr <210> SEQ ID NO 66 <211> LENGTH: 242 <212> TYPE: PRT <213> ORGANISM: Unknown <220> FEATURE: <223> OTHER INFORMATION: one of six Madagascan enterovirus isolates, not neutralizable by PDVM pools, therefore untypeable <400> SEQUENCE: 66 Tyr Thr Tyr Arg Thr Ala Pro Pro Arg Ile Ser Ile Pro Tyr Ile Gly 1 5 10 15 Ile Ala Asn Ala Tyr Ser His Phe Tyr Asp Gly Tyr Ala Val Val Pro 20 25 30 Leu Lys Asp Ser Ala Gln Asp Ser Gly Ala Ala Tyr Tyr Gly Ala Val 35 40 45 Ser Ile Asn Asp Phe Gly Leu Leu Ala Val Arg Val Val Asn Glu His 50 55 60 Asn Pro Val Arg Val Ser Ser Lys Val Arg Val Tyr Met Lys Pro Lys 65 70 75 80 His Val Arg Val Trp Cys Pro Arg Pro Pro Arg Ala Val Glu Tyr Tyr 85 90 95 Gly Pro Gly Val Asp Tyr Lys Ala Asp Thr Leu Thr Pro Leu Pro Thr 100 105 110 Lys Asn Leu Thr Thr Tyr Gly Phe Gly His Gln Asn Lys Ala Ile Tyr 115 120 125 Val Ala Gly Tyr Lys Ile Val Asn Tyr His Leu Ala Thr Ala Glu Asp 130 135 140 Phe Arg His Cys Val Arg Ser Met Trp Glu Arg Asp Ile Met Ile Val 145 150 155 160 Glu Ser Lys Ala Gln Gly Arg Asp Gln Ile Ala Arg Cys Ala Cys Arg 165 170 175 Ser Gly Val Tyr Tyr Cys Glu Ser Arg Gly Lys His Tyr Pro Ile Thr 180 185 190 Ile Thr Gln Pro Thr Phe Gln Trp Met Glu Lys Asn Asp Phe Tyr Pro 195 200 205 Ala Arg Tyr Gln Ser His Met Thr Ile Gly His Gly Phe Ala Ala Pro 210 215 220 Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His Gly Val Met Gly Leu 225 230 235 240 Ile Thr <210> SEQ ID NO 67 <211> LENGTH: 747 <212> TYPE: DNA <213> ORGANISM: Unknown <220> FEATURE: <223> OTHER INFORMATION: one of six Madagascan enterovirus isolates, not neutralizable by PDVM pools, therefore untypeable <221> NAME/KEY: CDS <222> LOCATION: (1)..(747) <223> OTHER INFORMATION: <400> SEQUENCE: 67 tac act tat ggt aca gca cca cct agg att tca ata cct tac atc ggt 48 Tyr Thr Tyr Gly Thr Ala Pro Pro Arg Ile Ser Ile Pro Tyr Ile Gly 1 5 10 15 atc gcc aat gct tac tcc cac ttc tac gat ggg tat gca gtt gtg cca 96 Ile Ala Asn Ala Tyr Ser His Phe Tyr Asp Gly Tyr Ala Val Val Pro 20 25 30 ctg aaa gat tcc aca cta gac tca gga gct gcg tat tat ggt gca gtg 144 Leu Lys Asp Ser Thr Leu Asp Ser Gly Ala Ala Tyr Tyr Gly Ala Val 35 40 45 tcc att aac gac ttt ggc ctt ctt gca gtt cga gtg gtc aat gag cac 192 Ser Ile Asn Asp Phe Gly Leu Leu Ala Val Arg Val Val Asn Glu His 50 55 60 aac cct gtc aga gta tca tca aag gtt aga gta tac atg aaa cca aaa 240 Asn Pro Val Arg Val Ser Ser Lys Val Arg Val Tyr Met Lys Pro Lys 65 70 75 80 cat gtc aga gtg tgg tgt cca cgc cca cca cgt gcc gtg gag tat tac 288 His Val Arg Val Trp Cys Pro Arg Pro Pro Arg Ala Val Glu Tyr Tyr 85 90 95 gga cct gga gtg gat tac aag gca aac aca tta aca cca cta cca acc 336 Gly Pro Gly Val Asp Tyr Lys Ala Asn Thr Leu Thr Pro Leu Pro Thr 100 105 110 aag aat cta acc aca tat ggt ttt ggt cac caa aac aag gca ata tat 384 Lys Asn Leu Thr Thr Tyr Gly Phe Gly His Gln Asn Lys Ala Ile Tyr 115 120 125 gtt gca ggg tat aag ata gtg aat tac cac ttg gcc act gca gag gat 432 Val Ala Gly Tyr Lys Ile Val Asn Tyr His Leu Ala Thr Ala Glu Asp 130 135 140 ttt agg cac tgt gtt aga agt atg tgg gag cgt gac atc atg gtg gtg 480 Phe Arg His Cys Val Arg Ser Met Trp Glu Arg Asp Ile Met Val Val 145 150 155 160 gaa tcc aaa gca cag ggg acc gac caa att gcc cga tgt gcg tgt cga 528 Glu Ser Lys Ala Gln Gly Thr Asp Gln Ile Ala Arg Cys Ala Cys Arg 165 170 175 gca ggt gta tat ttc tgt gaa tca aga gga aaa cac tat ccc ata aca 576 Ala Gly Val Tyr Phe Cys Glu Ser Arg Gly Lys His Tyr Pro Ile Thr 180 185 190 att aca cag cct acc ttc cag tgg atg gag aaa aat gat ttc tac cca 624 Ile Thr Gln Pro Thr Phe Gln Trp Met Glu Lys Asn Asp Phe Tyr Pro 195 200 205 gca cgc tac cag tca cac atg act atc ggt tgt ggt tac gca gcc cct 672 Ala Arg Tyr Gln Ser His Met Thr Ile Gly Cys Gly Tyr Ala Ala Pro 210 215 220 gga gac tgt ggt ggt att ttg cga tgt caa cac ggt gtc atg ggc ctg 720 Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His Gly Val Met Gly Leu 225 230 235 240 ata acc gcc ggt ggt gat ggg ctg gtg 747 Ile Thr Ala Gly Gly Asp Gly Leu Val 245 <210> SEQ ID NO 68 <211> LENGTH: 249 <212> TYPE: PRT <213> ORGANISM: Unknown <220> FEATURE: <223> OTHER INFORMATION: one of six Madagascan enterovirus isolates, not neutralizable by PDVM pools, therefore untypeable <400> SEQUENCE: 68 Tyr Thr Tyr Gly Thr Ala Pro Pro Arg Ile Ser Ile Pro Tyr Ile Gly 1 5 10 15 Ile Ala Asn Ala Tyr Ser His Phe Tyr Asp Gly Tyr Ala Val Val Pro 20 25 30 Leu Lys Asp Ser Thr Leu Asp Ser Gly Ala Ala Tyr Tyr Gly Ala Val 35 40 45 Ser Ile Asn Asp Phe Gly Leu Leu Ala Val Arg Val Val Asn Glu His 50 55 60 Asn Pro Val Arg Val Ser Ser Lys Val Arg Val Tyr Met Lys Pro Lys 65 70 75 80 His Val Arg Val Trp Cys Pro Arg Pro Pro Arg Ala Val Glu Tyr Tyr 85 90 95 Gly Pro Gly Val Asp Tyr Lys Ala Asn Thr Leu Thr Pro Leu Pro Thr 100 105 110 Lys Asn Leu Thr Thr Tyr Gly Phe Gly His Gln Asn Lys Ala Ile Tyr 115 120 125 Val Ala Gly Tyr Lys Ile Val Asn Tyr His Leu Ala Thr Ala Glu Asp 130 135 140 Phe Arg His Cys Val Arg Ser Met Trp Glu Arg Asp Ile Met Val Val 145 150 155 160 Glu Ser Lys Ala Gln Gly Thr Asp Gln Ile Ala Arg Cys Ala Cys Arg 165 170 175 Ala Gly Val Tyr Phe Cys Glu Ser Arg Gly Lys His Tyr Pro Ile Thr 180 185 190 Ile Thr Gln Pro Thr Phe Gln Trp Met Glu Lys Asn Asp Phe Tyr Pro 195 200 205 Ala Arg Tyr Gln Ser His Met Thr Ile Gly Cys Gly Tyr Ala Ala Pro 210 215 220 Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His Gly Val Met Gly Leu 225 230 235 240 Ile Thr Ala Gly Gly Asp Gly Leu Val 245 <210> SEQ ID NO 69 <211> LENGTH: 813 <212> TYPE: DNA <213> ORGANISM: Unknown <220> FEATURE: <223> OTHER INFORMATION: one of six Madagascan enterovirus isolates, not neutralizable by PDVM pools, therefore untypeable <221> NAME/KEY: CDS <222> LOCATION: (1)..(813) <223> OTHER INFORMATION: <400> SEQUENCE: 69 gta ttc tac act tac ggg act gct ccc gcg aga att tcc ata ccg tac 48 Val Phe Tyr Thr Tyr Gly Thr Ala Pro Ala Arg Ile Ser Ile Pro Tyr 1 5 10 15 gtg ggg atc gct aac gca tat tct cac ttt tat gat gga ttt gct act 96 Val Gly Ile Ala Asn Ala Tyr Ser His Phe Tyr Asp Gly Phe Ala Thr 20 25 30 gtt cct att gaa gga gtg acc aca gac cca ggt gca gca tat tac ggg 144 Val Pro Ile Glu Gly Val Thr Thr Asp Pro Gly Ala Ala Tyr Tyr Gly 35 40 45 gca act tcc atc aat gat ttc ggg att cta gcg gtg agg gtg gtg aat 192 Ala Thr Ser Ile Asn Asp Phe Gly Ile Leu Ala Val Arg Val Val Asn 50 55 60 gac cac aac cca gtt cag gtg tct tca aag atc aga gtt tac atg aaa 240 Asp His Asn Pro Val Gln Val Ser Ser Lys Ile Arg Val Tyr Met Lys 65 70 75 80 cca aag cat gtg cgc gtg tgg tgc cca agg cca cca aga gct gtc cca 288 Pro Lys His Val Arg Val Trp Cys Pro Arg Pro Pro Arg Ala Val Pro 85 90 95 tac ttc ggt ccg ggt gtg gac tac aag gga gat gcc ttg acc ccc tta 336 Tyr Phe Gly Pro Gly Val Asp Tyr Lys Gly Asp Ala Leu Thr Pro Leu 100 105 110 tca aca aag tct cta acc act tat gga ttt gga cat caa aat aaa gca 384 Ser Thr Lys Ser Leu Thr Thr Tyr Gly Phe Gly His Gln Asn Lys Ala 115 120 125 gtg tac acg gcg ggc tac aag att tgt aac tac cac ctt gca acc cag 432 Val Tyr Thr Ala Gly Tyr Lys Ile Cys Asn Tyr His Leu Ala Thr Gln 130 135 140 gag gat ctg tca aat gca gtg aat atc atg tgg aac aga gat ctt tta 480 Glu Asp Leu Ser Asn Ala Val Asn Ile Met Trp Asn Arg Asp Leu Leu 145 150 155 160 gtg aca gaa tcc aaa gcc cag ggc acg gat tcc ata gct aga tgt act 528 Val Thr Glu Ser Lys Ala Gln Gly Thr Asp Ser Ile Ala Arg Cys Thr 165 170 175 tgc aat gct gga gtg tac tac tgt gag tct aga cga aag tat tac ccc 576 Cys Asn Ala Gly Val Tyr Tyr Cys Glu Ser Arg Arg Lys Tyr Tyr Pro 180 185 190 gtt tcc ttc att ggt cca acc ttc caa tac atg gaa gca aat gat tac 624 Val Ser Phe Ile Gly Pro Thr Phe Gln Tyr Met Glu Ala Asn Asp Tyr 195 200 205 tac cca gcc cgg tat caa tct cac atg cta atc ggc tat ggc ttt gcc 672 Tyr Pro Ala Arg Tyr Gln Ser His Met Leu Ile Gly Tyr Gly Phe Ala 210 215 220 tcg cct gga gac tgt ggc ggt atc ctc aga tgt caa cat gga gtt att 720 Ser Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His Gly Val Ile 225 230 235 240 gga atc atc aca gct gga ggg gaa ggc cta gtg gcc ttt tca gac atc 768 Gly Ile Ile Thr Ala Gly Gly Glu Gly Leu Val Ala Phe Ser Asp Ile 245 250 255 aga gat ctt tat gca tat gaa gag gaa gcc atg gag caa ggc atc 813 Arg Asp Leu Tyr Ala Tyr Glu Glu Glu Ala Met Glu Gln Gly Ile 260 265 270 <210> SEQ ID NO 70 <211> LENGTH: 271 <212> TYPE: PRT <213> ORGANISM: Unknown <220> FEATURE: <223> OTHER INFORMATION: one of six Madagascan enterovirus isolates, not neutralizable by PDVM pools, therefore untypeable <400> SEQUENCE: 70 Val Phe Tyr Thr Tyr Gly Thr Ala Pro Ala Arg Ile Ser Ile Pro Tyr 1 5 10 15 Val Gly Ile Ala Asn Ala Tyr Ser His Phe Tyr Asp Gly Phe Ala Thr 20 25 30 Val Pro Ile Glu Gly Val Thr Thr Asp Pro Gly Ala Ala Tyr Tyr Gly 35 40 45 Ala Thr Ser Ile Asn Asp Phe Gly Ile Leu Ala Val Arg Val Val Asn 50 55 60 Asp His Asn Pro Val Gln Val Ser Ser Lys Ile Arg Val Tyr Met Lys 65 70 75 80 Pro Lys His Val Arg Val Trp Cys Pro Arg Pro Pro Arg Ala Val Pro 85 90 95 Tyr Phe Gly Pro Gly Val Asp Tyr Lys Gly Asp Ala Leu Thr Pro Leu 100 105 110 Ser Thr Lys Ser Leu Thr Thr Tyr Gly Phe Gly His Gln Asn Lys Ala 115 120 125 Val Tyr Thr Ala Gly Tyr Lys Ile Cys Asn Tyr His Leu Ala Thr Gln 130 135 140 Glu Asp Leu Ser Asn Ala Val Asn Ile Met Trp Asn Arg Asp Leu Leu 145 150 155 160 Val Thr Glu Ser Lys Ala Gln Gly Thr Asp Ser Ile Ala Arg Cys Thr 165 170 175 Cys Asn Ala Gly Val Tyr Tyr Cys Glu Ser Arg Arg Lys Tyr Tyr Pro 180 185 190 Val Ser Phe Ile Gly Pro Thr Phe Gln Tyr Met Glu Ala Asn Asp Tyr 195 200 205 Tyr Pro Ala Arg Tyr Gln Ser His Met Leu Ile Gly Tyr Gly Phe Ala 210 215 220 Ser Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His Gly Val Ile 225 230 235 240 Gly Ile Ile Thr Ala Gly Gly Glu Gly Leu Val Ala Phe Ser Asp Ile 245 250 255 Arg Asp Leu Tyr Ala Tyr Glu Glu Glu Ala Met Glu Gln Gly Ile 260 265 270 <210> SEQ ID NO 71 <211> LENGTH: 786 <212> TYPE: DNA <213> ORGANI SM: Coxsackievirus B <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(786) <223> OTHER INFORMATION: <400> SEQUENCE: 71 tgg acc gaa ggt aac gca cca cct cga atg tct gta ccc ttc atg agt 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Val Pro Phe Met Ser 1 5 10 15 atc gga aac gcc tat agc acc ttt tat gac ggt tgg tca gat ttt tca 96 Ile Gly Asn Ala Tyr Ser Thr Phe Tyr Asp Gly Trp Ser Asp Phe Ser 20 25 30 tct aag gga gtg tat ggt ttg aac acc ctg aat aat atg ggg tca ttg 144 Ser Lys Gly Val Tyr Gly Leu Asn Thr Leu Asn Asn Met Gly Ser Leu 35 40 45 tac att cgg cac gtc aat ggg ccc aac cca gtg cct atc act agt acc 192 Tyr Ile Arg His Val Asn Gly Pro Asn Pro Val Pro Ile Thr Ser Thr 50 55 60 ata cgt att tac ctc aag cca aaa cat gtt aaa gca tgg gtg cct cga 240 Ile Arg Ile Tyr Leu Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 cca cca aga ttg tgt caa tat gaa aca tca agg caa gtc aac ttc aat 288 Pro Pro Arg Leu Cys Gln Tyr Glu Thr Ser Arg Gln Val Asn Phe Asn 85 90 95 gtg aca ggc gtg act gag tcc aga gac aac ata act acc atg aaa acc 336 Val Thr Gly Val Thr Glu Ser Arg Asp Asn Ile Thr Thr Met Lys Thr 100 105 110 acc gga gcc ttc ggt caa cag tct ggg gca gcg tat gtt ggc aac tat 384 Thr Gly Ala Phe Gly Gln Gln Ser Gly Ala Ala Tyr Val Gly Asn Tyr 115 120 125 agg gtg gtt aat aga cac ttg gcg acc cac acc gac ggg caa aat tgc 432 Arg Val Val Asn Arg His Leu Ala Thr His Thr Asp Gly Gln Asn Cys 130 135 140 att tgg gag gac tac aac agg gac ctt ctt gtg agc aca acc acg gcg 480 Ile Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala 145 150 155 160 cat gga tgc gac act ata gca ggg tgc cag tgc acc acc ggc gtg tac 528 His Gly Cys Asp Thr Ile Ala Gly Cys Gln Cys Thr Thr Gly Val Tyr 165 170 175 ttc tgt gct tca aag aac aaa cat tac ccc gtg agt ttc gaa gga cca 576 Phe Cys Ala Ser Lys Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro 180 185 190 ggg ctg gtg gag gtt cag aag agc gaa tat tac ccc aag aga tat caa 624 Gly Leu Val Glu Val Gln Lys Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln 195 200 205 tcc cac gtg ctc cta gct gct ggg ttc tca gaa cca ggt gac tgt ggt 672 Ser His Val Leu Leu Ala Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly 210 215 220 ggt att tta aga tgc gag cac ggt gtc ata ggt ttg gtg acc atg gga 720 Gly Ile Leu Arg Cys Glu His Gly Val Ile Gly Leu Val Thr Met Gly 225 230 235 240 gga aag ggc gtt gtt ggc ttt gct gat gtt cgg gac tta ata tgg ctg 768 Gly Lys Gly Val Val Gly Phe Ala Asp Val Arg Asp Leu Ile Trp Leu 245 250 255 gag gat gat gct atg gaa 786 Glu Asp Asp Ala Met Glu 260 <210> SEQ ID NO 72 <211> LENGTH: 262 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus B <400> SEQUENCE: 72 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Val Pro Phe Met Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Thr Phe Tyr Asp Gly Trp Ser Asp Phe Ser 20 25 30 Ser Lys Gly Val Tyr Gly Leu Asn Thr Leu Asn Asn Met Gly Ser Leu 35 40 45 Tyr Ile Arg His Val Asn Gly Pro Asn Pro Val Pro Ile Thr Ser Thr 50 55 60 Ile Arg Ile Tyr Leu Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Gln Tyr Glu Thr Ser Arg Gln Val Asn Phe Asn 85 90 95 Val Thr Gly Val Thr Glu Ser Arg Asp Asn Ile Thr Thr Met Lys Thr 100 105 110 Thr Gly Ala Phe Gly Gln Gln Ser Gly Ala Ala Tyr Val Gly Asn Tyr 115 120 125 Arg Val Val Asn Arg His Leu Ala Thr His Thr Asp Gly Gln Asn Cys 130 135 140 Ile Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala 145 150 155 160 His Gly Cys Asp Thr Ile Ala Gly Cys Gln Cys Thr Thr Gly Val Tyr 165 170 175 Phe Cys Ala Ser Lys Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro 180 185 190 Gly Leu Val Glu Val Gln Lys Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln 195 200 205 Ser His Val Leu Leu Ala Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly 210 215 220 Gly Ile Leu Arg Cys Glu His Gly Val Ile Gly Leu Val Thr Met Gly 225 230 235 240 Gly Lys Gly Val Val Gly Phe Ala Asp Val Arg Asp Leu Ile Trp Leu 245 250 255 Glu Asp Asp Ala Met Glu 260 <210> SEQ ID NO 73 <211> LENGTH: 699 <212> TYPE: DNA <213> ORGANISM: Unknown <220> FEATURE: <223> OTHER INFORMATION: one of six Madagascan enterovirus isolates, not neutralizable by PDVM pools, therefore untypeable <221> NAME/KEY: CDS <222> LOCATION: (1)..(699) <223> OTHER INFORMATION: <400> SEQUENCE: 73 ata ccg tac gtg ggg att gct aac gca tat tct aac ttt tat gat gga 48 Ile Pro Tyr Val Gly Ile Ala Asn Ala Tyr Ser Asn Phe Tyr Asp Gly 1 5 10 15 tgt gct act gtt ccc att gaa gga gag acc aca gac cca tgt gca gca 96 Cys Ala Thr Val Pro Ile Glu Gly Glu Thr Thr Asp Pro Cys Ala Ala 20 25 30 tat tac ggg gca act tcc atc aat gac ttc ggg att cta gcg gtg agg 144 Tyr Tyr Gly Ala Thr Ser Ile Asn Asp Phe Gly Ile Leu Ala Val Arg 35 40 45 gtg gtg aat gaa cac aac cca gtt cag gtg tct tca aag atc aga gtt 192 Val Val Asn Glu His Asn Pro Val Gln Val Ser Ser Lys Ile Arg Val 50 55 60 tac atg aaa cca aag cat gtg cgc gtg tgg tgc cca agg cca cca aga 240 Tyr Met Lys Pro Lys His Val Arg Val Trp Cys Pro Arg Pro Pro Arg 65 70 75 80 gct gtc cca tac ttt ggc ccg gga gtg gac tac aag gga gat gcc ttg 288 Ala Val Pro Tyr Phe Gly Pro Gly Val Asp Tyr Lys Gly Asp Ala Leu 85 90 95 acc ccc tta tca aca aag tct cta acc act tat gga ttt gga cat caa 336 Thr Pro Leu Ser Thr Lys Ser Leu Thr Thr Tyr Gly Phe Gly His Gln 100 105 110 aac aaa gca gtg tac acg gca ggc tac aag att tgc aac tac cac ctt 384 Asn Lys Ala Val Tyr Thr Ala Gly Tyr Lys Ile Cys Asn Tyr His Leu 115 120 125 gca acc cag gaa gat ctg tca aat gca gtg aat gtc atg tgg aac aga 432 Ala Thr Gln Glu Asp Leu Ser Asn Ala Val Asn Val Met Trp Asn Arg 130 135 140 gat ctt ttg gtg aca gaa tcc aaa gcc cag ggc aca gat tcc ata gct 480 Asp Leu Leu Val Thr Glu Ser Lys Ala Gln Gly Thr Asp Ser Ile Ala 145 150 155 160 aga tgt act tgc aat gct gga gtg tac tac tgt gag tct aga cga aag 528 Arg Cys Thr Cys Asn Ala Gly Val Tyr Tyr Cys Glu Ser Arg Arg Lys 165 170 175 tat tac ccc gtt tcc ttc att ggt cca acc ttt caa tac atg gaa gca 576 Tyr Tyr Pro Val Ser Phe Ile Gly Pro Thr Phe Gln Tyr Met Glu Ala 180 185 190 aat gat tac tat cca gcc cgg tat caa tcc cac atg cta atc ggc tat 624 Asn Asp Tyr Tyr Pro Ala Arg Tyr Gln Ser His Met Leu Ile Gly Tyr 195 200 205 ggc ttt gcc tcg cct gga gac tgt ggc ggt atc ctc aga tgt caa cat 672 Gly Phe Ala Ser Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His 210 215 220 gga gtt att gga atc atc aca gct gga 699 Gly Val Ile Gly Ile Ile Thr Ala Gly 225 230 <210> SEQ ID NO 74 <211> LENGTH: 233 <212> TYPE: PRT <213> ORGANISM: Unknown <220> FEATURE: <223> OTHER INFORMATION: one of six Madagascan enterovirus isolates, not neutralizable by PDVM pools, therefore untypeable <400> SEQUENCE: 74 Ile Pro Tyr Val Gly Ile Ala Asn Ala Tyr Ser Asn Phe Tyr Asp Gly 1 5 10 15 Cys Ala Thr Val Pro Ile Glu Gly Glu Thr Thr Asp Pro Cys Ala Ala 20 25 30 Tyr Tyr Gly Ala Thr Ser Ile Asn Asp Phe Gly Ile Leu Ala Val Arg 35 40 45 Val Val Asn Glu His Asn Pro Val Gln Val Ser Ser Lys Ile Arg Val 50 55 60 Tyr Met Lys Pro Lys His Val Arg Val Trp Cys Pro Arg Pro Pro Arg 65 70 75 80 Ala Val Pro Tyr Phe Gly Pro Gly Val Asp Tyr Lys Gly Asp Ala Leu 85 90 95 Thr Pro Leu Ser Thr Lys Ser Leu Thr Thr Tyr Gly Phe Gly His Gln 100 105 110 Asn Lys Ala Val Tyr Thr Ala Gly Tyr Lys Ile Cys Asn Tyr His Leu 115 120 125 Ala Thr Gln Glu Asp Leu Ser Asn Ala Val Asn Val Met Trp Asn Arg 130 135 140 Asp Leu Leu Val Thr Glu Ser Lys Ala Gln Gly Thr Asp Ser Ile Ala 145 150 155 160 Arg Cys Thr Cys Asn Ala Gly Val Tyr Tyr Cys Glu Ser Arg Arg Lys 165 170 175 Tyr Tyr Pro Val Ser Phe Ile Gly Pro Thr Phe Gln Tyr Met Glu Ala 180 185 190 Asn Asp Tyr Tyr Pro Ala Arg Tyr Gln Ser His Met Leu Ile Gly Tyr 195 200 205 Gly Phe Ala Ser Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His 210 215 220 Gly Val Ile Gly Ile Ile Thr Ala Gly 225 230 <210> SEQ ID NO 75 <211> LENGTH: 783 <212> TYPE: DNA <213> ORGANISM: Unknown <220> FEATURE: <223> OTHER INFORMATION: one of six Madagascan enterovirus isolates, not neutralizable by PDVM pools, therefore untypeable <221> NAME/KEY: CDS <222> LOCATION: (1)..(783) <223> OTHER INFORMATION: <400> SEQUENCE: 75 ttc tac act tat ggc aca gca cca cct aga att tca ata cct tac atc 48 Phe Tyr Thr Tyr Gly Thr Ala Pro Pro Arg Ile Ser Ile Pro Tyr Ile 1 5 10 15 ggt atc gcc aat gct tac tct cac ttc tac gac ggg tat gca gtt gtg 96 Gly Ile Ala Asn Ala Tyr Ser His Phe Tyr Asp Gly Tyr Ala Val Val 20 25 30 ccg ctg aaa gat tcc aca caa gac tca gga gct gcg tat tat ggt gcg 144 Pro Leu Lys Asp Ser Thr Gln Asp Ser Gly Ala Ala Tyr Tyr Gly Ala 35 40 45 gtg tcc atc aat gac ttt ggc ctt ctt gca gtt cga gtg gtt aat gaa 192 Val Ser Ile Asn Asp Phe Gly Leu Leu Ala Val Arg Val Val Asn Glu 50 55 60 cac aac cct gtc aga gta tca tcg aag att aga gta tac atg aaa cca 240 His Asn Pro Val Arg Val Ser Ser Lys Ile Arg Val Tyr Met Lys Pro 65 70 75 80 aaa cat gtt agg gtg tgg tgt cca cgc cca cca cgt gcc gtg gag tac 288 Lys His Val Arg Val Trp Cys Pro Arg Pro Pro Arg Ala Val Glu Tyr 85 90 95 tac gga cct ggg gtg gat tac aag gca aac aca tta aca cca cta cca 336 Tyr Gly Pro Gly Val Asp Tyr Lys Ala Asn Thr Leu Thr Pro Leu Pro 100 105 110 acc aag aat cta acc aca tat ggc ttt ggt cac caa aac aag gca ata 384 Thr Lys Asn Leu Thr Thr Tyr Gly Phe Gly His Gln Asn Lys Ala Ile 115 120 125 tat gtg gca ggg tat aag ata gtg aat tac cac ttg gcc acg gca gaa 432 Tyr Val Ala Gly Tyr Lys Ile Val Asn Tyr His Leu Ala Thr Ala Glu 130 135 140 gat ttt agg cac tgt gtt aga agc atg tgg gag cgt gac atc atg gtg 480 Asp Phe Arg His Cys Val Arg Ser Met Trp Glu Arg Asp Ile Met Val 145 150 155 160 gtt gaa tcc aaa gcg cat ggg act gac caa att gct cga tgt gcg tgt 528 Val Glu Ser Lys Ala His Gly Thr Asp Gln Ile Ala Arg Cys Ala Cys 165 170 175 cgg act ggt gtg tat ttt tgt gaa tca aga gga aaa cat tat ccc ata 576 Arg Thr Gly Val Tyr Phe Cys Glu Ser Arg Gly Lys His Tyr Pro Ile 180 185 190 aca att aca caa cct act ttc cag tgg atg gag aaa aat gat ttc tac 624 Thr Ile Thr Gln Pro Thr Phe Gln Trp Met Glu Lys Asn Asp Phe Tyr 195 200 205 cca gca cgc tac caa tcg cac atg act atc ggt tgt ggt tac gca gcc 672 Pro Ala Arg Tyr Gln Ser His Met Thr Ile Gly Cys Gly Tyr Ala Ala 210 215 220 cct ggg gac tgt ggt ggt atc cta cgg tgt caa cac ggt gtc atg ggc 720 Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His Gly Val Met Gly 225 230 235 240 ctg ata acc gcc ggt ggc gat gga ctg gtg gca ttt gca gac gtc agg 768 Leu Ile Thr Ala Gly Gly Asp Gly Leu Val Ala Phe Ala Asp Val Arg 245 250 255 gat ctc tac gtt gaa 783 Asp Leu Tyr Val Glu 260 <210> SEQ ID NO 76 <211> LENGTH: 261 <212> TYPE: PRT <213> ORGANISM: Unknown <220> FEATURE: <223> OTHER INFORMATION: one of six Madagascan enterovirus isolates, not neutralizable by PDVM pools, therefore untypeable <400> SEQUENCE: 76 Phe Tyr Thr Tyr Gly Thr Ala Pro Pro Arg Ile Ser Ile Pro Tyr Ile 1 5 10 15 Gly Ile Ala Asn Ala Tyr Ser His Phe Tyr Asp Gly Tyr Ala Val Val 20 25 30 Pro Leu Lys Asp Ser Thr Gln Asp Ser Gly Ala Ala Tyr Tyr Gly Ala 35 40 45 Val Ser Ile Asn Asp Phe Gly Leu Leu Ala Val Arg Val Val Asn Glu 50 55 60 His Asn Pro Val Arg Val Ser Ser Lys Ile Arg Val Tyr Met Lys Pro 65 70 75 80 Lys His Val Arg Val Trp Cys Pro Arg Pro Pro Arg Ala Val Glu Tyr 85 90 95 Tyr Gly Pro Gly Val Asp Tyr Lys Ala Asn Thr Leu Thr Pro Leu Pro 100 105 110 Thr Lys Asn Leu Thr Thr Tyr Gly Phe Gly His Gln Asn Lys Ala Ile 115 120 125 Tyr Val Ala Gly Tyr Lys Ile Val Asn Tyr His Leu Ala Thr Ala Glu 130 135 140 Asp Phe Arg His Cys Val Arg Ser Met Trp Glu Arg Asp Ile Met Val 145 150 155 160 Val Glu Ser Lys Ala His Gly Thr Asp Gln Ile Ala Arg Cys Ala Cys 165 170 175 Arg Thr Gly Val Tyr Phe Cys Glu Ser Arg Gly Lys His Tyr Pro Ile 180 185 190 Thr Ile Thr Gln Pro Thr Phe Gln Trp Met Glu Lys Asn Asp Phe Tyr 195 200 205 Pro Ala Arg Tyr Gln Ser His Met Thr Ile Gly Cys Gly Tyr Ala Ala 210 215 220 Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Gln His Gly Val Met Gly 225 230 235 240 Leu Ile Thr Ala Gly Gly Asp Gly Leu Val Ala Phe Ala Asp Val Arg 245 250 255 Asp Leu Tyr Val Glu 260 <210> SEQ ID NO 77 <211> LENGTH: 705 <212> TYPE: DNA <213> ORGANISM: Coxsackievirus B <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(705) <223> OTHER INFORMATION: <400> SEQUENCE: 77 acg gaa ggg aat gct cca cca agg atg tca att cca ttt atc agc att 48 Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser Ile 1 5 10 15 ggc aat gcg tac agc tgt ttt tat gat ggt tgg acg cag ttt tca aga 96 Gly Asn Ala Tyr Ser Cys Phe Tyr Asp Gly Trp Thr Gln Phe Ser Arg 20 25 30 aat gga gtc tat ggg att aac act tta aat aac atg ggt act ttg tac 144 Asn Gly Val Tyr Gly Ile Asn Thr Leu Asn Asn Met Gly Thr Leu Tyr 35 40 45 atg agg cac gtt aac gag gca gga cag ggc cca att aag agc acc gtc 192 Met Arg His Val Asn Glu Ala Gly Gln Gly Pro Ile Lys Ser Thr Val 50 55 60 aga ata tac ttc aaa ccc aaa cac gtg aag gcg tgg gtg cct cga ccg 240 Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg Pro 65 70 75 80 cca agg ctg tgt cag tac gag aaa caa aag aat gtc aac ttc aac ccc 288 Pro Arg Leu Cys Gln Tyr Glu Lys Gln Lys Asn Val Asn Phe Asn Pro 85 90 95 aca gga gtt acc aca agc aga cta aac atc aca aca aca ggt gcc ttt 336 Thr Gly Val Thr Thr Ser Arg Leu Asn Ile Thr Thr Thr Gly Ala Phe 100 105 110 ggt cag caa tcg ggt gcc gta tac gtt ggt aac tac agg att gtg aac 384 Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Ile Val Asn 115 120 125 agg cac ctt gct aca cac aat gac tgg caa aat tgt gtg tgg gag gat 432 Arg His Leu Ala Thr His Asn Asp Trp Gln Asn Cys Val Trp Glu Asp 130 135 140 tat aat aga gac ctt ctc gtg agc acc act acg gcg cac ggg tgt gac 480 Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp 145 150 155 160 acc att gcc agg tgc cga tgt acg tca ggt gtg tac ttt tgt gcc tcc 528 Thr Ile Ala Arg Cys Arg Cys Thr Ser Gly Val Tyr Phe Cys Ala Ser 165 170 175 aag aac aga cac tac cct gta atc ttt gag gga cct ggc ctt gct gaa 576 Lys Asn Arg His Tyr Pro Val Ile Phe Glu Gly Pro Gly Leu Ala Glu 180 185 190 gtc caa gaa agt gaa tac tac cct aaa cgg tac caa tca cat gtg ctt 624 Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu 195 200 205 ctt gca gca ggc ttc tct gag cct gga gat tgt gga ggc atc ttg agg 672 Leu Ala Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg 210 215 220 tgt gaa cat ggc gtt att ggt att gtg acc atg 705 Cys Glu His Gly Val Ile Gly Ile Val Thr Met 225 230 235 <210> SEQ ID NO 78 <211> LENGTH: 235 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus B <400> SEQUENCE: 78 Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser Ile 1 5 10 15 Gly Asn Ala Tyr Ser Cys Phe Tyr Asp Gly Trp Thr Gln Phe Ser Arg 20 25 30 Asn Gly Val Tyr Gly Ile Asn Thr Leu Asn Asn Met Gly Thr Leu Tyr 35 40 45 Met Arg His Val Asn Glu Ala Gly Gln Gly Pro Ile Lys Ser Thr Val 50 55 60 Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg Pro 65 70 75 80 Pro Arg Leu Cys Gln Tyr Glu Lys Gln Lys Asn Val Asn Phe Asn Pro 85 90 95 Thr Gly Val Thr Thr Ser Arg Leu Asn Ile Thr Thr Thr Gly Ala Phe 100 105 110 Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg Ile Val Asn 115 120 125 Arg His Leu Ala Thr His Asn Asp Trp Gln Asn Cys Val Trp Glu Asp 130 135 140 Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp 145 150 155 160 Thr Ile Ala Arg Cys Arg Cys Thr Ser Gly Val Tyr Phe Cys Ala Ser 165 170 175 Lys Asn Arg His Tyr Pro Val Ile Phe Glu Gly Pro Gly Leu Ala Glu 180 185 190 Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu 195 200 205 Leu Ala Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg 210 215 220 Cys Glu His Gly Val Ile Gly Ile Val Thr Met 225 230 235 <210> SEQ ID NO 79 <211> LENGTH: 777 <212> TYPE: DNA <213> ORGANISM: Coxsackievirus B <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(777) <223> OTHER INFORMATION: <400> SEQUENCE: 79 gtc ttt tgg acc gag ggg aat gca cca cca aga atg tca atc cca ttc 48 Val Phe Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe 1 5 10 15 att agc att ggc aat gca tac agc tgt ttt tac gat ggg tgg acg caa 96 Ile Ser Ile Gly Asn Ala Tyr Ser Cys Phe Tyr Asp Gly Trp Thr Gln 20 25 30 ttt tcg aga aat ggg gtc tac gga atc aat acc ctg aac aac atg ggc 144 Phe Ser Arg Asn Gly Val Tyr Gly Ile Asn Thr Leu Asn Asn Met Gly 35 40 45 act ctg tac atg cgg cat gtc aat gaa gca gga cag gga cca atc aag 192 Thr Leu Tyr Met Arg His Val Asn Glu Ala Gly Gln Gly Pro Ile Lys 50 55 60 agt aca gtc aga ata tat ttc aag ccc aag cac gta aaa gcg tgg ata 240 Ser Thr Val Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Ile 65 70 75 80 cca cga cca cca agg ttg tgc cag tat gag aag caa aag aat gtc aac 288 Pro Arg Pro Pro Arg Leu Cys Gln Tyr Glu Lys Gln Lys Asn Val Asn 85 90 95 ttt aac ccc aca gga gtc acc gag agc agg ctg aat att acg aca acc 336 Phe Asn Pro Thr Gly Val Thr Glu Ser Arg Leu Asn Ile Thr Thr Thr 100 105 110 ggg gtt ttt ggg cag caa tca ggg gct gtg tac gtg ggg aac tac aaa 384 Gly Val Phe Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Lys 115 120 125 gtg gta aac aga cat ttg gcg act cac cat gat tgg caa aac tgc gtg 432 Val Val Asn Arg His Leu Ala Thr His His Asp Trp Gln Asn Cys Val 130 135 140 tgg gaa gat tac aac agg gat cta ctt gtt agc aca acc aca gcc cat 480 Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His 145 150 155 160 ggg tgc gac aca ata gcc aga tgc cat tgt aca tct gga gtg tat ttc 528 Gly Cys Asp Thr Ile Ala Arg Cys His Cys Thr Ser Gly Val Tyr Phe 165 170 175 tgc aac agt aga aat aag cac tat ccg gtt tcg ttt gaa ggc ccg ggg 576 Cys Asn Ser Arg Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro Gly 180 185 190 tta gtt gag gtt cag gaa agt gaa tat tac cca aaa aga tac caa tcc 624 Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser 195 200 205 cac gtg ctc ctt gca acc ggg ttt tcc aag cca ggc gat tgc ggt ggc 672 His Val Leu Leu Ala Thr Gly Phe Ser Lys Pro Gly Asp Cys Gly Gly 210 215 220 atc tta agg tgc gag cat ggt gtc att ggg cta gtg act atg gga gga 720 Ile Leu Arg Cys Glu His Gly Val Ile Gly Leu Val Thr Met Gly Gly 225 230 235 240 gag ggt gtc gtt ggc ttt gct gac gtg cgc gaa ctt ctg tgg tta gag 768 Glu Gly Val Val Gly Phe Ala Asp Val Arg Glu Leu Leu Trp Leu Glu 245 250 255 gat gat gca 777 Asp Asp Ala <210> SEQ ID NO 80 <211> LENGTH: 259 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus B <400> SEQUENCE: 80 Val Phe Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe 1 5 10 15 Ile Ser Ile Gly Asn Ala Tyr Ser Cys Phe Tyr Asp Gly Trp Thr Gln 20 25 30 Phe Ser Arg Asn Gly Val Tyr Gly Ile Asn Thr Leu Asn Asn Met Gly 35 40 45 Thr Leu Tyr Met Arg His Val Asn Glu Ala Gly Gln Gly Pro Ile Lys 50 55 60 Ser Thr Val Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Ile 65 70 75 80 Pro Arg Pro Pro Arg Leu Cys Gln Tyr Glu Lys Gln Lys Asn Val Asn 85 90 95 Phe Asn Pro Thr Gly Val Thr Glu Ser Arg Leu Asn Ile Thr Thr Thr 100 105 110 Gly Val Phe Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Lys 115 120 125 Val Val Asn Arg His Leu Ala Thr His His Asp Trp Gln Asn Cys Val 130 135 140 Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His 145 150 155 160 Gly Cys Asp Thr Ile Ala Arg Cys His Cys Thr Ser Gly Val Tyr Phe 165 170 175 Cys Asn Ser Arg Asn Lys His Tyr Pro Val Ser Phe Glu Gly Pro Gly 180 185 190 Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser 195 200 205 His Val Leu Leu Ala Thr Gly Phe Ser Lys Pro Gly Asp Cys Gly Gly 210 215 220 Ile Leu Arg Cys Glu His Gly Val Ile Gly Leu Val Thr Met Gly Gly 225 230 235 240 Glu Gly Val Val Gly Phe Ala Asp Val Arg Glu Leu Leu Trp Leu Glu 245 250 255 Asp Asp Ala <210> SEQ ID NO 81 <211> LENGTH: 651 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(651) <223> OTHER INFORMATION: <400> SEQUENCE: 81 tgg aca gag ggc aac gcc ccc ccg cgt atg tcc atc cca ttt ata agt 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 ata ggg aat gcg tac agt aat ttt tat gac gga tgg tca cac ttt tca 96 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 caa aac ggg gta tat ggg tac aac gca tta aac aac atg ggc aag ttg 144 Gln Asn Gly Val Tyr Gly Tyr Asn Ala Leu Asn Asn Met Gly Lys Leu 35 40 45 tac gca cga cac gtg aat aaa gat aca cca tac caa atg tct agc acg 192 Tyr Ala Arg His Val Asn Lys Asp Thr Pro Tyr Gln Met Ser Ser Thr 50 55 60 atc cgc gtg tac ttt aag ccc aag cac atc aga gtc tgg gtg ccg cga 240 Ile Arg Val Tyr Phe Lys Pro Lys His Ile Arg Val Trp Val Pro Arg 65 70 75 80 cca ccg cgc ttg tgt cct tat att aag tct agt aat gtt aat ttt gaa 288 Pro Pro Arg Leu Cys Pro Tyr Ile Lys Ser Ser Asn Val Asn Phe Glu 85 90 95 ccg acc aac cta act gac tca aga tca agc ata aca tac gtg ccc gat 336 Pro Thr Asn Leu Thr Asp Ser Arg Ser Ser Ile Thr Tyr Val Pro Asp 100 105 110 act atc cgt cca gaa gtt cgc aca gcc ggc aag ttt ggt caa cag tct 384 Thr Ile Arg Pro Glu Val Arg Thr Ala Gly Lys Phe Gly Gln Gln Ser 115 120 125 ggc gca atc tac gtg gga aat tat aga gtg gtg aac aga cat ctt gcc 432 Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 act cac agc gac tgg caa aat tgt gtg tgg gaa gaa tat aac aga gac 480 Thr His Ser Asp Trp Gln Asn Cys Val Trp Glu Glu Tyr Asn Arg Asp 145 150 155 160 ctc ctc gtg agt acc acc aca gcc cat gga tgt gac acc att gcc aga 528 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 tgc cag tgc acg aca ggt gtg tac ttt tgt gcc tcg aga aac aaa cat 576 Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His 180 185 190 tat cca gtc acc ttt gaa gga cca ggt ttg gta gaa gtc cag gag agt 624 Tyr Pro Val Thr Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 gaa tac tac ccc aag aga tat cag tcc 651 Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser 210 215 <210> SEQ ID NO 82 <211> LENGTH: 217 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 82 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Ile Ser 1 5 10 15 Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Asn Gly Val Tyr Gly Tyr Asn Ala Leu Asn Asn Met Gly Lys Leu 35 40 45 Tyr Ala Arg His Val Asn Lys Asp Thr Pro Tyr Gln Met Ser Ser Thr 50 55 60 Ile Arg Val Tyr Phe Lys Pro Lys His Ile Arg Val Trp Val Pro Arg 65 70 75 80 Pro Pro Arg Leu Cys Pro Tyr Ile Lys Ser Ser Asn Val Asn Phe Glu 85 90 95 Pro Thr Asn Leu Thr Asp Ser Arg Ser Ser Ile Thr Tyr Val Pro Asp 100 105 110 Thr Ile Arg Pro Glu Val Arg Thr Ala Gly Lys Phe Gly Gln Gln Ser 115 120 125 Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala 130 135 140 Thr His Ser Asp Trp Gln Asn Cys Val Trp Glu Glu Tyr Asn Arg Asp 145 150 155 160 Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg 165 170 175 Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His 180 185 190 Tyr Pro Val Thr Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser 195 200 205 Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser 210 215 <210> SEQ ID NO 83 <211> LENGTH: 660 <212> TYPE: DNA <213> ORGANISM: Coxsackievirus B <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(660) <223> OTHER INFORMATION: <400> SEQUENCE: 83 att ctc acc cac caa ata atg tat gtg cca cca ggt ggc cca gta cca 48 Ile Leu Thr His Gln Ile Met Tyr Val Pro Pro Gly Gly Pro Val Pro 1 5 10 15 gat aag gtt gac tca tat gtg tgg cag aca tcc act aat ccc agt gtt 96 Asp Lys Val Asp Ser Tyr Val Trp Gln Thr Ser Thr Asn Pro Ser Val 20 25 30 ttc tgg acc gaa ggc aat gcg ccg cca cgc atg tcc att cca ttt ttg 144 Phe Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Leu 35 40 45 agc att ggc aat gca tat tcg aac ttt tat gat ggc tgg tcg gag ttt 192 Ser Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser Glu Phe 50 55 60 gcc aga agc ggg gtg tat ggc ata aac act ttg aac aac atg ggg acc 240 Ala Arg Ser Gly Val Tyr Gly Ile Asn Thr Leu Asn Asn Met Gly Thr 65 70 75 80 ctt tac gca aga cat gtg aat gct ggg agc aca gga cca ata aaa agc 288 Leu Tyr Ala Arg His Val Asn Ala Gly Ser Thr Gly Pro Ile Lys Ser 85 90 95 act att aga atc tat ttt aaa cca aag cac gtt aag gcg tgg ata cct 336 Thr Ile Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Ile Pro 100 105 110 aga cct cca agg ctg tgt cag tat gag aaa gca aag aat gtt aat ttt 384 Arg Pro Pro Arg Leu Cys Gln Tyr Glu Lys Ala Lys Asn Val Asn Phe 115 120 125 caa cct agt ggt gta aca aca acc aga cag agc atc aca gca atg aca 432 Gln Pro Ser Gly Val Thr Thr Thr Arg Gln Ser Ile Thr Ala Met Thr 130 135 140 aac act ggg gcg ttc ggg caa caa tcg ggg gca ata tac gtg ggc aac 480 Asn Thr Gly Ala Phe Gly Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn 145 150 155 160 tat aga gtg gtg aat aga cac ctg gcc aca ttc acg gac tgg cag aat 528 Tyr Arg Val Val Asn Arg His Leu Ala Thr Phe Thr Asp Trp Gln Asn 165 170 175 tgt gtg tgg gaa gac tac aac agg gat cta ctt gtt agt act act acg 576 Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr 180 185 190 gcc cat gga tgt gat gtc ata gct aga tgc cag tgc aca ttg ggt gta 624 Ala His Gly Cys Asp Val Ile Ala Arg Cys Gln Cys Thr Leu Gly Val 195 200 205 tac tac tgt gcc tcg aaa aat aag cac tat cca gtc 660 Tyr Tyr Cys Ala Ser Lys Asn Lys His Tyr Pro Val 210 215 220 <210> SEQ ID NO 84 <211> LENGTH: 220 <212> TYPE: PRT <213> ORGANISM: Coxsackievirus B <400> SEQUENCE: 84 Ile Leu Thr His Gln Ile Met Tyr Val Pro Pro Gly Gly Pro Val Pro 1 5 10 15 Asp Lys Val Asp Ser Tyr Val Trp Gln Thr Ser Thr Asn Pro Ser Val 20 25 30 Phe Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Leu 35 40 45 Ser Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser Glu Phe 50 55 60 Ala Arg Ser Gly Val Tyr Gly Ile Asn Thr Leu Asn Asn Met Gly Thr 65 70 75 80 Leu Tyr Ala Arg His Val Asn Ala Gly Ser Thr Gly Pro Ile Lys Ser 85 90 95 Thr Ile Arg Ile Tyr Phe Lys Pro Lys His Val Lys Ala Trp Ile Pro 100 105 110 Arg Pro Pro Arg Leu Cys Gln Tyr Glu Lys Ala Lys Asn Val Asn Phe 115 120 125 Gln Pro Ser Gly Val Thr Thr Thr Arg Gln Ser Ile Thr Ala Met Thr 130 135 140 Asn Thr Gly Ala Phe Gly Gln Gln Ser Gly Ala Ile Tyr Val Gly Asn 145 150 155 160 Tyr Arg Val Val Asn Arg His Leu Ala Thr Phe Thr Asp Trp Gln Asn 165 170 175 Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr 180 185 190 Ala His Gly Cys Asp Val Ile Ala Arg Cys Gln Cys Thr Leu Gly Val 195 200 205 Tyr Tyr Cys Ala Ser Lys Asn Lys His Tyr Pro Val 210 215 220 <210> SEQ ID NO 85 <211> LENGTH: 744 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(744) <223> OTHER INFORMATION: <400> SEQUENCE: 85 tgg aca gaa ggc aat gcc cct ccc agg atg tcg ata cca ttc atg agt 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 gtt ggt aac gca tat tgc aac ttt tat gat gga tgg tct cat ttc agc 96 Val Gly Asn Ala Tyr Cys Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 cag agc ggt gtg tac gga tac acc act ttg aac aac atg gga cac ctg 144 Gln Ser Gly Val Tyr Gly Tyr Thr Thr Leu Asn Asn Met Gly His Leu 35 40 45 tat ttt aga cat gtg aac aaa tca act gca tat cca gtt aac agt gtt 192 Tyr Phe Arg His Val Asn Lys Ser Thr Ala Tyr Pro Val Asn Ser Val 50 55 60 gcc cgc gtc tat ttt aag ccc aag cac gtg aag gca tgg gtg cct cgt 240 Ala Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 gcg cca cgc ttg tgc cca tat ctg tat gca aga aat gtc aac ttt gat 288 Ala Pro Arg Leu Cys Pro Tyr Leu Tyr Ala Arg Asn Val Asn Phe Asp 85 90 95 gtg caa ggg gta acc gaa tct cga ggc aaa att acc ctt gac cga tca 336 Val Gln Gly Val Thr Glu Ser Arg Gly Lys Ile Thr Leu Asp Arg Ser 100 105 110 act cac aat cct ttg tca aac act ggt gtt ttt ggg caa caa tca ggg 384 Thr His Asn Pro Leu Ser Asn Thr Gly Val Phe Gly Gln Gln Ser Gly 115 120 125 gct gca tat gtg ggc aac tac aga gtt gtc aac aga cac ctc gcc act 432 Ala Ala Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala Thr 130 135 140 cat gct gac cgg caa aat tgc gtg tgg gag gac tac aat aga gac ttg 480 His Ala Asp Arg Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu 145 150 155 160 ttg gtt agc acc acc aca gcc cac ggg tgt gac acc ata gcc agg tgc 528 Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys 165 170 175 cag tgc tcc aca ggt gtg tat ttt tgc gca tcc agg aac aaa cat tac 576 Gln Cys Ser Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His Tyr 180 185 190 cca gtg tca ttt gaa ggg ccc ggg ctc gtc gag gtg cag gag agt gag 624 Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu 195 200 205 tat tac cca aga agg tac caa tca cat gtg ttg ctc gcc gca ggg ttt 672 Tyr Tyr Pro Arg Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly Phe 210 215 220 tct gag cca ggt gac tgt ggg ggc atc ttg aga tgc gag cat ggc gtc 720 Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly Val 225 230 235 240 ata ggg ctt gtg acc atg ggg ggc 744 Ile Gly Leu Val Thr Met Gly Gly 245 <210> SEQ ID NO 86 <211> LENGTH: 248 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 86 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 Val Gly Asn Ala Tyr Cys Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Ser Gly Val Tyr Gly Tyr Thr Thr Leu Asn Asn Met Gly His Leu 35 40 45 Tyr Phe Arg His Val Asn Lys Ser Thr Ala Tyr Pro Val Asn Ser Val 50 55 60 Ala Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 Ala Pro Arg Leu Cys Pro Tyr Leu Tyr Ala Arg Asn Val Asn Phe Asp 85 90 95 Val Gln Gly Val Thr Glu Ser Arg Gly Lys Ile Thr Leu Asp Arg Ser 100 105 110 Thr His Asn Pro Leu Ser Asn Thr Gly Val Phe Gly Gln Gln Ser Gly 115 120 125 Ala Ala Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala Thr 130 135 140 His Ala Asp Arg Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu 145 150 155 160 Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys 165 170 175 Gln Cys Ser Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His Tyr 180 185 190 Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu 195 200 205 Tyr Tyr Pro Arg Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly Phe 210 215 220 Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly Val 225 230 235 240 Ile Gly Leu Val Thr Met Gly Gly 245 <210> SEQ ID NO 87 <211> LENGTH: 744 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(744) <223> OTHER INFORMATION: <400> SEQUENCE: 87 tgg aca gaa ggc aat gcc cct ccc agg atg tcg ata cca ttc atg agt 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 gtt ggt aac gca tat tgc aac ttt tat gat gga tgg tct cat ttc agc 96 Val Gly Asn Ala Tyr Cys Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 cag agc ggt gtg tac gga tac act act ttg aac aac atg gga cac ctg 144 Gln Ser Gly Val Tyr Gly Tyr Thr Thr Leu Asn Asn Met Gly His Leu 35 40 45 tat ttt aga cat gtg aac aaa tca act gca tat cca gtt aac agt gtt 192 Tyr Phe Arg His Val Asn Lys Ser Thr Ala Tyr Pro Val Asn Ser Val 50 55 60 gcc cgc gtc tat ttt aag ccc aag cac gtg aag gca tgg gtg cct cgt 240 Ala Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 gcg cca cgc ttg tgc cca tat ctg tat gca aga aat gtc aac ttt gat 288 Ala Pro Arg Leu Cys Pro Tyr Leu Tyr Ala Arg Asn Val Asn Phe Asp 85 90 95 gtg caa ggg gta acc gaa tct cgg ggc aaa att acc ctt gac cga tca 336 Val Gln Gly Val Thr Glu Ser Arg Gly Lys Ile Thr Leu Asp Arg Ser 100 105 110 act cac aat cct ttg tca aac act ggt gtt ttt ggg caa caa tca ggg 384 Thr His Asn Pro Leu Ser Asn Thr Gly Val Phe Gly Gln Gln Ser Gly 115 120 125 gct gca tat gtg ggc aac tac aga gtt gtc aac aga cac ctc gcc act 432 Ala Ala Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala Thr 130 135 140 cat gct gac cgg cag aat tgc gtg tgg gag gac tac aat aga gac ttg 480 His Ala Asp Arg Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu 145 150 155 160 ttg gtt agc acc acc aca gcc cac ggg tgt gac acc ata gcc agg tgc 528 Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys 165 170 175 cag tgc tcc aca ggt gtg tat ttt tgc gca tcc agg aac aaa cat tac 576 Gln Cys Ser Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His Tyr 180 185 190 cca gtg tca ttt gaa ggg ccc ggg ctc gtc gag gtg cag gag agt gag 624 Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu 195 200 205 tat tac cca aga agg tac caa tca cat gtg ttg ctc gcc gca ggg ttt 672 Tyr Tyr Pro Arg Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly Phe 210 215 220 tct gag cca ggt gac tgt ggg ggc atc ttg aga tgc gag cat ggc gtc 720 Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly Val 225 230 235 240 ata ggg ctt gtg acc atg ggg ggc 744 Ile Gly Leu Val Thr Met Gly Gly 245 <210> SEQ ID NO 88 <211> LENGTH: 248 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 88 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 Val Gly Asn Ala Tyr Cys Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Ser Gly Val Tyr Gly Tyr Thr Thr Leu Asn Asn Met Gly His Leu 35 40 45 Tyr Phe Arg His Val Asn Lys Ser Thr Ala Tyr Pro Val Asn Ser Val 50 55 60 Ala Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 Ala Pro Arg Leu Cys Pro Tyr Leu Tyr Ala Arg Asn Val Asn Phe Asp 85 90 95 Val Gln Gly Val Thr Glu Ser Arg Gly Lys Ile Thr Leu Asp Arg Ser 100 105 110 Thr His Asn Pro Leu Ser Asn Thr Gly Val Phe Gly Gln Gln Ser Gly 115 120 125 Ala Ala Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala Thr 130 135 140 His Ala Asp Arg Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu 145 150 155 160 Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys 165 170 175 Gln Cys Ser Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His Tyr 180 185 190 Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu 195 200 205 Tyr Tyr Pro Arg Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly Phe 210 215 220 Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly Val 225 230 235 240 Ile Gly Leu Val Thr Met Gly Gly 245 <210> SEQ ID NO 89 <211> LENGTH: 744 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(744) <223> OTHER INFORMATION: <400> SEQUENCE: 89 tgg aca gaa ggc aat gcc cct ccc agg atg tcg ata cca ttc atg agt 48 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 gtt ggt aac gca tat tgc aac ttt tat gat gga tgg tct cat ttc agc 96 Val Gly Asn Ala Tyr Cys Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 cag agc ggt gtg tac gga tac act act ttg aac aac atg gga cac ctg 144 Gln Ser Gly Val Tyr Gly Tyr Thr Thr Leu Asn Asn Met Gly His Leu 35 40 45 tat ttt aga cat gtg aac aaa tca act gca tat cca gtt aac agt gtt 192 Tyr Phe Arg His Val Asn Lys Ser Thr Ala Tyr Pro Val Asn Ser Val 50 55 60 gcc cgc gtc tat ttt aag ccc aag cac gtg aag gca tgg gtg cct cgt 240 Ala Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 gcg cca cgc ttg tgc cca tat ctg tat gca aga aat gtc aac ttt gat 288 Ala Pro Arg Leu Cys Pro Tyr Leu Tyr Ala Arg Asn Val Asn Phe Asp 85 90 95 gtg caa ggg gta acc gaa tct cgg ggc aaa att acc ctt gac cga tca 336 Val Gln Gly Val Thr Glu Ser Arg Gly Lys Ile Thr Leu Asp Arg Ser 100 105 110 act cac aat cct ttg tca aac act ggt gtt ttt ggg caa caa tca ggg 384 Thr His Asn Pro Leu Ser Asn Thr Gly Val Phe Gly Gln Gln Ser Gly 115 120 125 gct gca tat gtg ggc aac tac aga gtt gtc aac aga cac ctc gcc act 432 Ala Ala Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala Thr 130 135 140 cat gct gac tgg cag aat tgc gtg tgg gag gac tac aat aga gac ttg 480 His Ala Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu 145 150 155 160 ttg gtt agc acc acc aca gcc cac ggg tgt gac acc ata gcc agg tgc 528 Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys 165 170 175 cag tgc tcc aca ggt gtg tat ttt tgc gca tcc agg aac aaa cat tac 576 Gln Cys Ser Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His Tyr 180 185 190 cca gtg tca ttt gaa ggg ccc ggg ctc gtc gag gtg cag gag agt gag 624 Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu 195 200 205 tat tac cca aga agg tac caa tca cat gtg ttg ctc gcc gca ggg ttt 672 Tyr Tyr Pro Arg Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly Phe 210 215 220 tct gag cca ggt gac tgt ggg ggc atc ttg aga tgc gag cat ggc gtc 720 Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly Val 225 230 235 240 ata ggg ctt gtg acc atg ggg ggc 744 Ile Gly Leu Val Thr Met Gly Gly 245 <210> SEQ ID NO 90 <211> LENGTH: 248 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 90 Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe Met Ser 1 5 10 15 Val Gly Asn Ala Tyr Cys Asn Phe Tyr Asp Gly Trp Ser His Phe Ser 20 25 30 Gln Ser Gly Val Tyr Gly Tyr Thr Thr Leu Asn Asn Met Gly His Leu 35 40 45 Tyr Phe Arg His Val Asn Lys Ser Thr Ala Tyr Pro Val Asn Ser Val 50 55 60 Ala Arg Val Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val Pro Arg 65 70 75 80 Ala Pro Arg Leu Cys Pro Tyr Leu Tyr Ala Arg Asn Val Asn Phe Asp 85 90 95 Val Gln Gly Val Thr Glu Ser Arg Gly Lys Ile Thr Leu Asp Arg Ser 100 105 110 Thr His Asn Pro Leu Ser Asn Thr Gly Val Phe Gly Gln Gln Ser Gly 115 120 125 Ala Ala Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His Leu Ala Thr 130 135 140 His Ala Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Asn Arg Asp Leu 145 150 155 160 Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile Ala Arg Cys 165 170 175 Gln Cys Ser Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn Lys His Tyr 180 185 190 Pro Val Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln Glu Ser Glu 195 200 205 Tyr Tyr Pro Arg Arg Tyr Gln Ser His Val Leu Leu Ala Ala Gly Phe 210 215 220 Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu His Gly Val 225 230 235 240 Ile Gly Leu Val Thr Met Gly Gly 245 <210> SEQ ID NO 91 <211> LENGTH: 747 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(747) <223> OTHER INFORMATION: <400> SEQUENCE: 91 atc ttt tgg aca gag ggg aac gca cca cct agg atg tct att cct ttc 48 Ile Phe Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe 1 5 10 15 att agc att ggc aac gcg tat agc aac ttc tat gat ggt tgg tca cac 96 Ile Ser Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His 20 25 30 ttc tcc caa aac ggt gtg tac ggg tat aac aca ctt aat cac atg ggc 144 Phe Ser Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly 35 40 45 cag att tac gtc agg cac gtg aac ggg tct tcc cca ctg cct atg act 192 Gln Ile Tyr Val Arg His Val Asn Gly Ser Ser Pro Leu Pro Met Thr 50 55 60 agc aca gtc agg atg tac ttc aaa cca aag cat gta aag gca tgg gtc 240 Ser Thr Val Arg Met Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val 65 70 75 80 cca cgc ccg cct aga ttg tgt cag tac aag aac gca gca acc gtc aac 288 Pro Arg Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ala Thr Val Asn 85 90 95 ttt aca cca acc aac atc acc gaa aag agg aat gac atc aat tac ata 336 Phe Thr Pro Thr Asn Ile Thr Glu Lys Arg Asn Asp Ile Asn Tyr Ile 100 105 110 cca gag aca gtc aga ccg gac gtc gca acg cat ggg gcg ttt ggc cag 384 Pro Glu Thr Val Arg Pro Asp Val Ala Thr His Gly Ala Phe Gly Gln 115 120 125 cag tca ggt gct gca tat gtt ggc aac tat agg ata gta aac agg cat 432 Gln Ser Gly Ala Ala Tyr Val Gly Asn Tyr Arg Ile Val Asn Arg His 130 135 140 tta gct acc cgc act gat tgg agg aac tgt gtg tgg gaa gac tac aat 480 Leu Ala Thr Arg Thr Asp Trp Arg Asn Cys Val Trp Glu Asp Tyr Asn 145 150 155 160 agg gac ctt cta gtg agc act act acg gca cac ggc tgt gac acc att 528 Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile 165 170 175 gcc aga tgc caa tgc aca acg ggt gtc tat ttc tgt gca tcg aag aac 576 Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Lys Asn 180 185 190 aaa cac tat cca gta cat ttt gaa gga ccg gga ctg gta gaa gtt caa 624 Lys His Tyr Pro Val His Phe Glu Gly Pro Gly Leu Val Glu Val Gln 195 200 205 gag agt gag tat tac cca aag aga tac caa tca cac gtg ctg tta gct 672 Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala 210 215 220 gcg ggg ttt tct gaa ccg gga gac tgt gga ggg att ctt aga tgc gaa 720 Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu 225 230 235 240 cac gga gtt atc ggc ctt gtc acc atg 747 His Gly Val Ile Gly Leu Val Thr Met 245 <210> SEQ ID NO 92 <211> LENGTH: 249 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 92 Ile Phe Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe 1 5 10 15 Ile Ser Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His 20 25 30 Phe Ser Gln Asn Gly Val Tyr Gly Tyr Asn Thr Leu Asn His Met Gly 35 40 45 Gln Ile Tyr Val Arg His Val Asn Gly Ser Ser Pro Leu Pro Met Thr 50 55 60 Ser Thr Val Arg Met Tyr Phe Lys Pro Lys His Val Lys Ala Trp Val 65 70 75 80 Pro Arg Pro Pro Arg Leu Cys Gln Tyr Lys Asn Ala Ala Thr Val Asn 85 90 95 Phe Thr Pro Thr Asn Ile Thr Glu Lys Arg Asn Asp Ile Asn Tyr Ile 100 105 110 Pro Glu Thr Val Arg Pro Asp Val Ala Thr His Gly Ala Phe Gly Gln 115 120 125 Gln Ser Gly Ala Ala Tyr Val Gly Asn Tyr Arg Ile Val Asn Arg His 130 135 140 Leu Ala Thr Arg Thr Asp Trp Arg Asn Cys Val Trp Glu Asp Tyr Asn 145 150 155 160 Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile 165 170 175 Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Lys Asn 180 185 190 Lys His Tyr Pro Val His Phe Glu Gly Pro Gly Leu Val Glu Val Gln 195 200 205 Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala 210 215 220 Ala Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu Arg Cys Glu 225 230 235 240 His Gly Val Ile Gly Leu Val Thr Met 245 <210> SEQ ID NO 93 <211> LENGTH: 645 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(645) <223> OTHER INFORMATION: <400> SEQUENCE: 93 gtg tcc tgg acc gag ggt aat gcc ccg cct agg ata tca ata cca ttt 48 Val Ser Trp Thr Glu Gly Asn Ala Pro Pro Arg Ile Ser Ile Pro Phe 1 5 10 15 ata agt gtg gga aat gcc tac agc tgt ttt tac gac ggc tgg tca cac 96 Ile Ser Val Gly Asn Ala Tyr Ser Cys Phe Tyr Asp Gly Trp Ser His 20 25 30 ttc tcg cag act gga gtt tat ggt tac aac aca ctt aat gat atg ggc 144 Phe Ser Gln Thr Gly Val Tyr Gly Tyr Asn Thr Leu Asn Asp Met Gly 35 40 45 caa ctg ttt gta agg cat gtg aac gag gca agt ccg ggt gct gtg tct 192 Gln Leu Phe Val Arg His Val Asn Glu Ala Ser Pro Gly Ala Val Ser 50 55 60 agt gtg gtg aga ata tat ttc aag ccc aaa cac atc aag gca tgg gta 240 Ser Val Val Arg Ile Tyr Phe Lys Pro Lys His Ile Lys Ala Trp Val 65 70 75 80 cct aga cca cca agg ttg tgc cag tat gtc aat gcc tct aca gta aac 288 Pro Arg Pro Pro Arg Leu Cys Gln Tyr Val Asn Ala Ser Thr Val Asn 85 90 95 ttc acg cca gag gga gtc acc aaa tct cga acg gac ttg atg act aca 336 Phe Thr Pro Glu Gly Val Thr Lys Ser Arg Thr Asp Leu Met Thr Thr 100 105 110 ggg gcc ttt ggt caa caa tct ggt gca gtg tat gta gga aat tac aga 384 Gly Ala Phe Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg 115 120 125 gtg gta aat aga cac ctg gct aca cac gca gac tgg caa aac tgc gtg 432 Val Val Asn Arg His Leu Ala Thr His Ala Asp Trp Gln Asn Cys Val 130 135 140 tgg gag gac tac aac agg gac ctc ctt gtg agc acc acc aca gcc cat 480 Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His 145 150 155 160 gga tgt gac gtc tta gcc agg tgc cag tgc acg aca ggg gtg tac ttc 528 Gly Cys Asp Val Leu Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe 165 170 175 tgc cag tcg agg aac aag cat tac cta att agt ttc gag ggt ccg gga 576 Cys Gln Ser Arg Asn Lys His Tyr Leu Ile Ser Phe Glu Gly Pro Gly 180 185 190 ctt gtc gag gtg cag gag agt gaa tac tat ccc aaa agg tac caa tct 624 Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser 195 200 205 cac gtg tta ctt gct gca ggc 645 His Val Leu Leu Ala Ala Gly 210 215 <210> SEQ ID NO 94 <211> LENGTH: 215 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 94 Val Ser Trp Thr Glu Gly Asn Ala Pro Pro Arg Ile Ser Ile Pro Phe 1 5 10 15 Ile Ser Val Gly Asn Ala Tyr Ser Cys Phe Tyr Asp Gly Trp Ser His 20 25 30 Phe Ser Gln Thr Gly Val Tyr Gly Tyr Asn Thr Leu Asn Asp Met Gly 35 40 45 Gln Leu Phe Val Arg His Val Asn Glu Ala Ser Pro Gly Ala Val Ser 50 55 60 Ser Val Val Arg Ile Tyr Phe Lys Pro Lys His Ile Lys Ala Trp Val 65 70 75 80 Pro Arg Pro Pro Arg Leu Cys Gln Tyr Val Asn Ala Ser Thr Val Asn 85 90 95 Phe Thr Pro Glu Gly Val Thr Lys Ser Arg Thr Asp Leu Met Thr Thr 100 105 110 Gly Ala Phe Gly Gln Gln Ser Gly Ala Val Tyr Val Gly Asn Tyr Arg 115 120 125 Val Val Asn Arg His Leu Ala Thr His Ala Asp Trp Gln Asn Cys Val 130 135 140 Trp Glu Asp Tyr Asn Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His 145 150 155 160 Gly Cys Asp Val Leu Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe 165 170 175 Cys Gln Ser Arg Asn Lys His Tyr Leu Ile Ser Phe Glu Gly Pro Gly 180 185 190 Leu Val Glu Val Gln Glu Ser Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser 195 200 205 His Val Leu Leu Ala Ala Gly 210 215 <210> SEQ ID NO 95 <211> LENGTH: 711 <212> TYPE: DNA <213> ORGANISM: Echovirus <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)..(711) <223> OTHER INFORMATION: <400> SEQUENCE: 95 atc ttc tgg act gaa ggt aac gcc ccc cca cgc atg tcc ata cca ttc 48 Ile Phe Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe 1 5 10 15 ata agc ata ggg aac gca tac agt aat ttt tat gat ggg tgg tct cat 96 Ile Ser Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His 20 25 30 ttc tca cag aat gga gtg tac ggg tac aat gca cta aac aac atg ggc 144 Phe Ser Gln Asn Gly Val Tyr Gly Tyr Asn Ala Leu Asn Asn Met Gly 35 40 45 aag tta tac gca cgt cat gtg aac aaa gac aca ccg tac caa atg tcc 192 Lys Leu Tyr Ala Arg His Val Asn Lys Asp Thr Pro Tyr Gln Met Ser 50 55 60 agc aca att cgc gtg tac ttc aaa ccc aaa cac gta agg gtt tgg gta 240 Ser Thr Ile Arg Val Tyr Phe Lys Pro Lys His Val Arg Val Trp Val 65 70 75 80 ccg cgc cca cca cgc ctg tgt cca tat att aag tcc agt aat gtc aac 288 Pro Arg Pro Pro Arg Leu Cys Pro Tyr Ile Lys Ser Ser Asn Val Asn 85 90 95 ttt gac cct act aat ttg acc gac caa aga cag agc ata acg tat gtg 336 Phe Asp Pro Thr Asn Leu Thr Asp Gln Arg Gln Ser Ile Thr Tyr Val 100 105 110 ccc gat act atc cgc cca gag gtt cgc aca gct ggt aag ttt ggt cag 384 Pro Asp Thr Ile Arg Pro Glu Val Arg Thr Ala Gly Lys Phe Gly Gln 115 120 125 caa tcc ggc gca att tac gta ggg aac tac agg gta gta aat aga cat 432 Gln Ser Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His 130 135 140 ctc gcc aca cat aat gac tgg caa aat tgt gtg tgg gag gac tat ggc 480 Leu Ala Thr His Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Gly 145 150 155 160 aga gat ctc ctt gtg agc act act acg gcg cac gga tgt gac acc ata 528 Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile 165 170 175 gcc agg tgc cag tgt acc aca gga gtg tac ttc tgt gcc tca agg aac 576 Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn 180 185 190 aaa cac tac ccg att tca ttt gaa ggg cca ggg ctg gtg gaa gtc cag 624 Lys His Tyr Pro Ile Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln 195 200 205 gaa aat gag tac tac ccc aag aga tac cag tct cat gta cta ttg gcc 672 Glu Asn Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala 210 215 220 gtt ggc ttc tcc gag ccg ggt gat tgt ggc ggc atc ctc 711 Val Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu 225 230 235 <210> SEQ ID NO 96 <211> LENGTH: 237 <212> TYPE: PRT <213> ORGANISM: Echovirus <400> SEQUENCE: 96 Ile Phe Trp Thr Glu Gly Asn Ala Pro Pro Arg Met Ser Ile Pro Phe 1 5 10 15 Ile Ser Ile Gly Asn Ala Tyr Ser Asn Phe Tyr Asp Gly Trp Ser His 20 25 30 Phe Ser Gln Asn Gly Val Tyr Gly Tyr Asn Ala Leu Asn Asn Met Gly 35 40 45 Lys Leu Tyr Ala Arg His Val Asn Lys Asp Thr Pro Tyr Gln Met Ser 50 55 60 Ser Thr Ile Arg Val Tyr Phe Lys Pro Lys His Val Arg Val Trp Val 65 70 75 80 Pro Arg Pro Pro Arg Leu Cys Pro Tyr Ile Lys Ser Ser Asn Val Asn 85 90 95 Phe Asp Pro Thr Asn Leu Thr Asp Gln Arg Gln Ser Ile Thr Tyr Val 100 105 110 Pro Asp Thr Ile Arg Pro Glu Val Arg Thr Ala Gly Lys Phe Gly Gln 115 120 125 Gln Ser Gly Ala Ile Tyr Val Gly Asn Tyr Arg Val Val Asn Arg His 130 135 140 Leu Ala Thr His Asn Asp Trp Gln Asn Cys Val Trp Glu Asp Tyr Gly 145 150 155 160 Arg Asp Leu Leu Val Ser Thr Thr Thr Ala His Gly Cys Asp Thr Ile 165 170 175 Ala Arg Cys Gln Cys Thr Thr Gly Val Tyr Phe Cys Ala Ser Arg Asn 180 185 190 Lys His Tyr Pro Ile Ser Phe Glu Gly Pro Gly Leu Val Glu Val Gln 195 200 205 Glu Asn Glu Tyr Tyr Pro Lys Arg Tyr Gln Ser His Val Leu Leu Ala 210 215 220 Val Gly Phe Ser Glu Pro Gly Asp Cys Gly Gly Ile Leu 225 230 235 

1. A purified polynucleotide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6.
 2. The purified polynucleotide of claim 1 which is SEQ ID NO:1.
 3. The purified polynucleotide of claim 1 which is SEQ ID NO:2.
 4. The purified polynucleotide of claim 1 which is SEQ ID NO:3.
 5. The purified polynucleotide of claim 1 which is SEQ ID NO:4.
 6. The purified polynucleotide of claim 1 which is SEQ ID NO:5.
 7. The purified polynucleotide of claim 1 which is SEQ ID NO:6.
 8. A purified polynucleotide selected from the group consisting of SEQ ID NOS:7-51.
 9. A purified polynucleotide consisting of a sequence which stringently hybridizes to one or more of the polynucleotides of claim 1, wherein said polynucleotide is from 15 to 25 nucleotides in length.
 10. A purified polynucleotide consisting of a sequence which stringently hybridizes to one or more of the polynucleotides of claim
 8. 11. A method of detecting an Enterovirus in a sample comprising contacting a polynucleotide region of a VP1 -2C genes of said enterovirus with amplification primers; amplifying said polynucleotide region of the VP1-2C gene; and detecting the presence of an amplified polynucleotide, wherein the presence of the amplified product is indicative of the presence of the Enterovirus in the sample.
 12. The method of claim 11, wherein the amplified polynucleotide comprises at least one sequence selected from the group consisting of SEQ ID NOS:7-51 or a sequence which stringently hybridizes to one or more of the sequences SEQ ID NOS:7-51.
 13. The method of claim 11, wherein said amplification primers are selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6.
 14. The method of claim 11, wherein said amplifying is RT-PCR.
 15. The method of claim 11, wherein said detecting comprises hybridization of said amplified product with a probe corresponding to the Enterovirus VP1-2C genes or a fragment thereof.
 16. The method of claim 15, wherein said probe is the 3′ third of the VP1 gene or a fragment thereof.
 17. The method of claim 15, wherein said probe is selected from the group consisting of SEQ ID NOS:7-51 and fragments thereof, sequences which stringently hybridize with SEQ ID NOS:7-51 and fragments thereof.
 18. The method of claim 15, wherein said detecting comprises serotyping the enterovirus with a probe specific for an enterovirus serotype.
 19. A purified polynucleotide hybridizing under stringent conditions with the amplified polynucleotide of the VP1-2C gene produced by the method of claim
 11. 20. A purified polypeptide encoded by the isolated polynucleotide of claim
 19. 21. A method of detecting the presence of an Enterovirus in a sample, comprising: contacting an purified and purified nucleic acid comprising (a) a polynucleotide sequence contained in the VP1-2C genes of said Enterovirus with the sample containing (b) an enterovirus; detecting the presence of hybridization between (a) and (b), wherein the presence of hybridization between (a) and (b) is indicative of the presence of the enterovirus in the sample.
 22. The method of claim 21, wherein purified and purified nucleic acid is SEQ ID NO:7-51 or fragments thereof or sequences which stringently hybridize to one or more of the sequences SEQ ID NOS:7-51 or fragments thereof.
 23. A method of detecting the presence of an Enterovirus in an Enterovirus sample, comprising contacting a polynucleotide region of VP1-2C genes of said enterovirus with amplification primers; amplifying said polynucleotide region of the VP1-2C genes; sequencing said amplified polynucleotide region; and comparing the sequence of said amplified polynucleotide region with known sequences of enteroviruses of various serotypes; wherein an identity score of at least 75% indicates that the two compared sequences are from enteroviruses of the same phenotype.
 24. The method of claim 23, wherein said amplification primers are selected from the group consisting of SEQ ID NO:1-6.
 25. The method of claim 23, wherein said amplifying is RT-PCR.
 26. A kit comprising one or more of the polynucleotides of claim
 1. 27. A kit comprising one or more of the polynucleotides of claim 8 or fragments thereof or the purified polynucleotides which stringently hybridize with SEQ ID NOS:7-51 or a fragment thereof.
 28. The kit of claim 26, further comprising one or more reagents necessary for RNA and/or DNA amplification.
 29. The kit of claim 28, further comprising one or more reagents necessary for RNA and/or DNA amplification.
 30. The kit of claim 26, further comprising one or more reagents necessary for RNA and/or DNA hybridization.
 31. The kit of claim 28, further comprising one or more reagents necessary for RNA and/or DNA hybridization.
 32. A purified polynucleotide hybridizing under stringent conditions with the amplified polynucleotide of a VP1-2C gene produced by the method of claim 11 and the two amplification primers.
 33. A purified polypeptide encoded by the purified polynucleotide of claim
 32. 